{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T15:43:40Z","timestamp":1761061420378,"version":"3.40.5"},"reference-count":29,"publisher":"S. Karger AG","issue":"3","license":[{"start":{"date-parts":[[2021,5,25]],"date-time":"2021-05-25T00:00:00Z","timestamp":1621900800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2021,5,25]],"date-time":"2021-05-25T00:00:00Z","timestamp":1621900800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Ophthalmologica"],"published-print":{"date-parts":[[2022]]},"abstract":"<b><i>Purpose:<\/i><\/b> The aim of this study was to investigate the influence of dietary supplementation using Age-Related Eye Disease Study 2 (AREDS2) on complement activation in patients with neovascular age-related macular degeneration (nAMD) under ongoing treatment. <b><i>Methods:<\/i><\/b> In this prospective, single-center, controlled, open-label investigator-initiated trial, eligible nAMD patients were randomized at a ratio of 1:1 in 2 groups: those with and without dietary AREDS2 supplementation for 4 weeks. Zinc, plasma, and aqueous humor (AH) complement levels were quantified via enzyme-linked immunosorbent assays. <b><i>Results:<\/i><\/b> Fifty of 62 enrolled patients completed the trial (AREDS2 <i>n<\/i> = 27, controls <i>n<\/i> = 23). Systemic zinc and complement levels were not different at baseline between the 2 groups (<i>p<\/i> &#x3e; 0.1). At the final visit, systemic zinc levels were significantly higher in the AREDS2 group (10.16 \u00b1 2.08 \u03bcmol\/L; 8.66 \u00b1 1.17 \u03bcmol\/L; <i>p<\/i> = 0.007), whereas systemic and AH complement levels were not different (<i>p<\/i> &#x3e; 0.1). In both groups, no significant change was observed in systemic levels of C3, C3a, FH, FI, and sC5b-9 (<i>p<\/i> &#x3e; 0.1). Only systemic complement component Ba showed an increase from baseline to the end visit (<i>p<\/i> = 0.01). This increase was higher in the control group (<i>p<\/i> = 0.02) than in the AREDS2 group (<i>p<\/i> = 0.23). <b><i>Conclusions:<\/i><\/b> Short-term dietary AREDS2 supplementation leads to a significant increase in systemic zinc levels without any influence on complement activation levels. <\/jats:p>","DOI":"10.1159\/000516885","type":"journal-article","created":{"date-parts":[[2021,5,25]],"date-time":"2021-05-25T21:00:32Z","timestamp":1621976432000},"page":"258-264","source":"Crossref","is-referenced-by-count":2,"title":["Ocular and Systemic Complement Activation during Anti-Vascular Endothelial Growth Factor Treatment and Age-Related Eye Disease Study 2 Dietary Supplementation in Neovascular Age-Related Macular Degeneration"],"prefix":"10.1159","volume":"245","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1088-3786","authenticated-orcid":false,"given":"Lebriz","family":"Altay","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julia","family":"Lemke","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jutta","family":"Schr\u00f6der-Braunstein","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Caroline","family":"Gietzelt","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vasilena","family":"Sitnilska","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Friederike","family":"Schaub","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claus","family":"Cursiefen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9527-4957","authenticated-orcid":false,"given":"Philip","family":"Enders","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sascha","family":"Fauser","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2021,5,25]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1001\/archopht.122.4.564"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1001\/archopht.119.10.1417"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1016\/j.ophtha.2008.01.036"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1016\/j.ophtha.2013.07.039"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1016\/j.ophtha.2014.05.008"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1016\/j.ophtha.2014.07.049"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1136\/bjophthalmol-2016-308624"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1718059115"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a017186"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1038\/ng.3448"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.222551899"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1016\/s0002-9394(02)01624-0"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1006\/exer.2001.1094"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0501536102"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1016\/j.molimm.2017.05.012"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0002593"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.09-3928"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.61.3.18"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1038\/eye.2016.328"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1038\/s41433-019-0501-4"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0112682"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1002\/mnfr.201801049"},{"key":"ref23","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M113.476143"},{"key":"ref24","doi-asserted-by":"publisher","DOI":"10.1038\/s41590-018-0295-8"},{"key":"ref25","doi-asserted-by":"publisher","DOI":"10.1155\/2018\/6872621"},{"key":"ref26","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-019-03419-4"},{"key":"ref27","doi-asserted-by":"publisher","DOI":"10.3390\/jcm3041234"},{"key":"ref28","doi-asserted-by":"publisher","DOI":"10.1016\/j.ophtha.2013.01.004"},{"key":"ref29","doi-asserted-by":"publisher","DOI":"10.1172\/JCI86418"}],"container-title":["Ophthalmologica"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/516885","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/516885","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T14:59:46Z","timestamp":1745420386000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000516885"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,5,25]]},"references-count":29,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2022,6,20]]},"published-print":{"date-parts":[[2022,6,28]]}},"URL":"https:\/\/doi.org\/10.1159\/000516885","archive":["Portico"],"relation":{},"ISSN":["0030-3755","1423-0267"],"issn-type":[{"type":"print","value":"0030-3755"},{"type":"electronic","value":"1423-0267"}],"subject":[],"published":{"date-parts":[[2021,5,25]]}}}