{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:52:18Z","timestamp":1787017938081,"version":"build-2736575974"},"reference-count":25,"publisher":"S. Karger AG","issue":"6","license":[{"start":{"date-parts":[[2021,1,1]],"date-time":"2021-01-01T00:00:00Z","timestamp":1609459200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2021,1,1]],"date-time":"2021-01-01T00:00:00Z","timestamp":1609459200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nephron"],"published-print":{"date-parts":[[2021]]},"abstract":"<jats:p>&lt;b&gt;&lt;i&gt;Background:&lt;\/i&gt;&lt;\/b&gt; We intended to investigate the function of circular RNA RasGEF domain family member 1B (circ_RASGEF1B) in lipopolysaccharide (LPS)-induced septic acute kidney injury (AKI) cell model and its associated mechanism. &lt;b&gt;&lt;i&gt;Methods:&lt;\/i&gt;&lt;\/b&gt; TCMK-1 cells were exposed to 10 \u03bcg\/mL LPS for 24 h to establish a septic AKI cell model. Mice were intraperitoneally injected with 10 mg\/kg LPS to establish a septic AKI mice model. Reverse transcription quantitative polymerase chain reaction (RT-qPCR) and Western blot assay were used to measure RNA and protein expression, respectively. Cell viability and apoptosis were assessed by 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and flow cytometry. Cell inflammatory response was analyzed using enzyme-linked immunosorbent assay. Dual-luciferase reporter assay was conducted to confirm the predicted target relationship between microRNA-146a-5p (miR-146a-5p) and circ_RASGEF1B or pyruvate dehydrogenase kinase 1 (Pdk1). &lt;b&gt;&lt;i&gt;Results:&lt;\/i&gt;&lt;\/b&gt; The circ_RASGEF1B level was upregulated in LPS-induced TCMK-1 cells and septic AKI mice models. LPS exposure reduced cell viability and promoted cell apoptosis and inflammatory response partly by upregulating circ_RASGEF1B. Circ_RASGEF1B bound to miR-146a-5p and miR-146a-5p interference partly overturned circ_RASGEF1B silencing-mediated effects in LPS-induced TCMK-1 cells. Pdk1 was a target of miR-146a-5p, and Pdk1 accumulation partly counteracted miR-146a-5p-induced influences in TCMK-1 cells upon LPS stimulation. &lt;b&gt;&lt;i&gt;Conclusion:&lt;\/i&gt;&lt;\/b&gt; Circ_RASGEF1B promoted LPS-induced apoptosis and inflammatory response in renal tubular epithelial cells partly by upregulating Pdk1 via acting as miR-146a-5p sponge.<\/jats:p>","DOI":"10.1159\/000517475","type":"journal-article","created":{"date-parts":[[2021,8,26]],"date-time":"2021-08-26T17:00:54Z","timestamp":1629997254000},"page":"748-759","source":"Crossref","is-referenced-by-count":14,"title":["Circ_RASGEF1B Promotes LPS-Induced Apoptosis and Inflammatory Response by Targeting MicroRNA-146a-5p\/Pdk1 Axis in Septic Acute Kidney Injury Cell Model"],"prefix":"10.1159","volume":"145","author":[{"given":"Jianghong","family":"Cao","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7059-2871","authenticated-orcid":false,"given":"Dongwu","family":"Shi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lili","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lu","family":"Song","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"127","published-online":{"date-parts":[[2021,8,26]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pharmthera.2012.01.004"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cnc.2016.08.001"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1186\/s13054-016-1581-1"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21010200"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1186\/s13054-016-1382-6"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00491.2018"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1016\/j.tox.2014.01.011"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1186\/s13098-021-00625-8"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1186\/s13054-016-1555-3"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.15741"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1038\/nature11993"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1159\/000501038"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.21037\/atm.2020.03.01"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1042\/BSR20200527"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.2147\/OTT.S213059"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.17821"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-biochem-060308-103103"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1007\/s10753-016-0498-9"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1590\/s0102-865020190060000002"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.26355\/eurrev_202005_21345"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1038\/nri2957"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1038\/nri2708"},{"key":"ref23","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2010.02.145"},{"key":"ref24","doi-asserted-by":"publisher","DOI":"10.1186\/s13046-019-1416-4"},{"key":"ref25","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-49480-8"}],"container-title":["Nephron"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/517475","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/517475","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T11:05:18Z","timestamp":1745406318000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000517475"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021]]},"references-count":25,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2021,11,11]]},"published-print":{"date-parts":[[2021,11,16]]}},"URL":"https:\/\/doi.org\/10.1159\/000517475","archive":["Portico"],"relation":{"is-supplemented-by":[{"id-type":"doi","id":"10.6084\/m9.figshare.16444086","asserted-by":"subject"}]},"ISSN":["1660-8151","2235-3186"],"issn-type":[{"value":"1660-8151","type":"print"},{"value":"2235-3186","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021]]}}}