{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T03:20:07Z","timestamp":1785468007350,"version":"3.56.0"},"reference-count":36,"publisher":"S. Karger AG","issue":"6","license":[{"start":{"date-parts":[[2021,1,1]],"date-time":"2021-01-01T00:00:00Z","timestamp":1609459200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2021,1,1]],"date-time":"2021-01-01T00:00:00Z","timestamp":1609459200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Acta Cytologica"],"published-print":{"date-parts":[[2021]]},"abstract":"<jats:p>&lt;b&gt;&lt;i&gt;Introduction\/Objective:&lt;\/i&gt;&lt;\/b&gt; Liquid-based cytology (LBC) is advantageous as multiple stained specimens can be prepared and used for additional assays such as immunocytochemical and molecular-pathological investigations. Two types of preservative-fixative solutions (fixatives) are used for nongynecologic specimens used in the BD SurePath-LBC (SP-LBC) method, and their components vary. However, few studies have evaluated the differences in antigen-retaining ability between these fixatives. Therefore, we investigated and compared the antigen-retaining ability of the fixatives in immunocytochemical staining (ICC) under long-term storage conditions. &lt;b&gt;&lt;i&gt;Materials and Methods:&lt;\/i&gt;&lt;\/b&gt; Sediments of cultured RAJI cells (derived from Burkitt\u2019s lymphoma) were added to each fixative (red and blue) and stored at room temperature for a specified period (1 h; 1 week; and 1, 3, and 6 months). The specimens were then prepared using the SP-LBC method and subjected to ICC. Positivity rate was calculated using the specimens fixed at room temperature for 1 h as a control. Antibodies against Ki67 expressed in the nucleus and against CD20 and leukocyte common antigen (LCA) expressed on the cell membrane were used. &lt;b&gt;&lt;i&gt;Results:&lt;\/i&gt;&lt;\/b&gt; For CD20 and LCA, the positivity rate increased with time in the red fixative compared with that in the control. In the blue fixative, the positivity rate was highest at 1 h and was maintained at a high level throughout the storage period. In contrast, the Ki67 positivity rate was highest at 1 h in both red and blue fixatives and markedly decreased with time. Therefore, although refrigerated (8\u00b0C) storage was used, no improvement was noted. &lt;b&gt;&lt;i&gt;Conclusions:&lt;\/i&gt;&lt;\/b&gt; Long-term storage is possible for cell membrane antigens at room temperature; however, it is unsuitable for intranuclear antigens. Therefore, we conclude that suitable fixative type and storage temperature differ based on antigen location. Further investigation is warranted. <\/jats:p>","DOI":"10.1159\/000518452","type":"journal-article","created":{"date-parts":[[2021,9,14]],"date-time":"2021-09-14T21:00:30Z","timestamp":1631653230000},"page":"510-521","source":"Crossref","is-referenced-by-count":5,"title":["Efficacy of the Antigenicity-Retaining Ability of Fixative Solutions for Liquid-Based Cytology: Immunocytochemistry of Long-Term Storage"],"prefix":"10.1159","volume":"65","author":[{"given":"Hiaki","family":"Sato","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshiaki","family":"Norimatsu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Satoshi","family":"Irino","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takeshi","family":"Nishikawa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"127","published-online":{"date-parts":[[2021,9,14]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1002\/(sici)1097-0339(199801)18:1%3c24::aid-dc5%3e3.0.co;2-u"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1002\/(sici)1097-0339(200001)22:1%3c52::aid-dc14%3e3.0.co;2-#"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1111\/cas.13549"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1159\/000353148"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1159\/000502108"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.4103\/JOC.JOC_61_18"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1002\/dc.23500"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.4103\/JOC.JOC_23_18"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.4103\/JOC.JOC_4_17"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.31557\/APJCP.2019.20.9.2611"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.25259\/Cytojournal_88_2019"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1186\/s12885-019-5492-9"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.21037\/jtd-20-2750"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.23495"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1002\/dc.21837"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1111\/cyt.12677"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1002\/cncy.21355"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1159\/000354960"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.3109\/10520299409106282"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymeth.2014.01.022"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1097\/00000478-200007000-00014"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1177\/6.5.303"},{"key":"ref23","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)64864-X"},{"key":"ref24","doi-asserted-by":"publisher","DOI":"10.1369\/0022155417741467"},{"key":"ref25","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2018.11.121"},{"key":"ref26","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellsig.2009.10.003"},{"key":"ref27","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.990130414"},{"key":"ref28","doi-asserted-by":"publisher","DOI":"10.1007\/s00412-018-0659-8"},{"key":"ref29","doi-asserted-by":"publisher","DOI":"10.4103\/1742-6413.75665"},{"key":"ref30","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.2007.047340"},{"key":"ref31","doi-asserted-by":"publisher","DOI":"10.1369\/0022155411423779"},{"key":"ref32","doi-asserted-by":"publisher","DOI":"10.1007\/s00418-015-1316-4"},{"key":"ref33","doi-asserted-by":"publisher","DOI":"10.3960\/jslrt.17017"},{"key":"ref34","doi-asserted-by":"publisher","DOI":"10.1177\/0300985813476067"},{"key":"ref35","doi-asserted-by":"publisher","DOI":"10.1097\/PAI.0000000000000756"},{"key":"ref36","doi-asserted-by":"publisher","DOI":"10.1038\/s41374-019-0366-y"}],"container-title":["Acta 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