{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,14]],"date-time":"2026-05-14T07:47:06Z","timestamp":1778744826418,"version":"3.51.4"},"reference-count":58,"publisher":"S. Karger AG","issue":"1","license":[{"start":{"date-parts":[[2021,9,10]],"date-time":"2021-09-10T00:00:00Z","timestamp":1631232000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2021,9,10]],"date-time":"2021-09-10T00:00:00Z","timestamp":1631232000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells Tissues Organs"],"published-print":{"date-parts":[[2022]]},"abstract":"<jats:p>At present, there is a growing interest in the study of the neurotropic activity of polyunsaturated fatty acid ethanolamides (N-acylethanolamines). N-docosahexaenoylethanolamine (DHEA), or synaptamide, an endogenous metabolite of docosahexaenoic acid, is a promising compound with anti-inflammatory activity. The results of this study demonstrate that synaptamide, when administered subcutaneously (4 mg\/kg\/day, 35 days), promotes a decrease in cold allodynia and mechanical hyperalgesia in a rat sciatic nerve chronic constriction injury (CCI) model. After CCI, synaptamide treatment enhanced the remyelination process in the site of sciatic nerve injury (33.4 \u00b1 1.1% in the CCI+Syn group, compared to 28.4 \u00b1 0.9% in the CCI group). Further, synaptamide suppressed the CCI-induced increase in the activity of microglia (13.1 \u00b1 0.5% in CCI+Syn, compared to 15.3 \u00b1 0.7% in the CCI group) and the number of nitric oxide synthase-positive neurons (58,307 \u00b1 5,206 cells\/mm&lt;sup&gt;3&lt;\/sup&gt; in CCI+Syn, compared to 80,288 \u00b1 4,287 cells\/mm&lt;sup&gt;3&lt;\/sup&gt; in the CCI group) in the dorsal horns of the spinal cord, and also reduced the concentration of interleukin 1 beta in the spinal cord (169.3 \u00b1 4 pg\/mg of protein in CCI+Syn, compared to 236.9 \u00b1 9.3 pg\/mg of protein in CCI group) 35 days after surgery. Synaptamide treatment resulted in decrease of reactive astrogliosis in the spinal cord dorsal horns to 20.8 \u00b1 1.3%, which occurred simultaneously with a decrease in the substance P (SP) level (9.8 \u00b1 0.5%) compared to vehicle-treated animals (30.2 \u00b1 2.2% and 13.4 \u00b1 0.9% of astroglia and SP staining area, respectively). In addition, synaptamide increased superoxide dismutase activity up to 68.6 \u00b1 0.8% (control 50.6 \u00b1 0.9%) in astrocyte culture. Thus, synaptamide provides anti-inflammatory and neuroprotective effects in both peripheral and central nervous system after sciatic nerve injury. <\/jats:p>","DOI":"10.1159\/000519376","type":"journal-article","created":{"date-parts":[[2021,9,10]],"date-time":"2021-09-10T21:00:32Z","timestamp":1631307632000},"page":"73-84","source":"Crossref","is-referenced-by-count":4,"title":["Analgesic Activity of Synaptamide in a Rat Sciatic Nerve Chronic Constriction Injury 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