{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T17:29:12Z","timestamp":1789752552045,"version":"4.0.1"},"reference-count":18,"publisher":"S. Karger AG","issue":"3","license":[{"start":{"date-parts":[[2022,1,1]],"date-time":"2022-01-01T00:00:00Z","timestamp":1640995200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2022,1,1]],"date-time":"2022-01-01T00:00:00Z","timestamp":1640995200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Obes Facts"],"published-print":{"date-parts":[[2022]]},"abstract":"<b><i>Objectives:<\/i><\/b> Bariatric surgery can treat obesity and T2DM, but the specific mechanism is unknown. This study investigated the effect and possible mechanism of duodenal-jejunal bypass (DJB) to treat T2DM. <b><i>Methods:<\/i><\/b> A T2DM rat model was established using a high-fat, high-sugar diet and a low dose of streptozotocin. DJB surgery and a sham operation (SO) were performed to analyze the effects on glucose homeostasis, lipid metabolism, and inflammation changes. Furthermore, the glucagon-like peptide-1 (GLP-1) in the ileum and the markers of endoplasmic reticulum stress (ERS) in the pancreas were examined after the surgery. The insulinoma cells (INS-1) were divided into three groups; group A was cultured with a normal sugar content (11.1 mmol\/L), group B was cultured with fluctuating high glucose (11.1 mmol\/L alternating with 33.3 mmol\/L), and group C was cultured with fluctuating high glucose and exendin-4 (100 nmol\/L). The cells were continuously cultured for 7 days in complete culture medium. The viability of the INS-1 cells was then investigated using the MTT method, apoptosis was detected by flow cytometry, and the ERS markers were detected by Western blot. <b><i>Results:<\/i><\/b> The blood glucose, lipids, insulin, and TNF-\u03b1 were significantly elevated in the T2DM model. A gradual recovery was observed in the DJB group. GLP-1 expression in the distal ileum of the DJB group was significantly higher than that in the T2DM control group (DM) and the SO group (<i>p<\/i> &#x3c; 0.05), and the markers of ERS expression in the pancreases of the DJB group decreased significantly more than those of groups DM and SO (<i>p<\/i> &#x3c; 0.05). Compared with group A, the cell viability in group B was decreased, and the ERS and apoptosis were increased (<i>p<\/i> &#x3c; 0.05). However, compared with group B, the cell viability in group C was improved, and the ERS and apoptosis declined (<i>p<\/i> &#x3c; 0.05). <b><i>Conclusions:<\/i><\/b> DJB can be used to treat T2DM in T2DM rats. The mechanism may be that the DJB stimulates the increased expression of GLP-1 on the far side of the ileum, and then, GLP-1 inhibits ERS in the pancreas, reducing the apoptosis of \u03b2 cells to create a treatment effect in the T2DM rats. <\/jats:p>","DOI":"10.1159\/000519417","type":"journal-article","created":{"date-parts":[[2022,3,17]],"date-time":"2022-03-17T22:00:40Z","timestamp":1647554440000},"page":"344-356","source":"Crossref","is-referenced-by-count":2,"title":["The Effect and Mechanism of Duodenal-Jejunal Bypass to Treat Type 2 Diabetes Mellitus in a Rat Model"],"prefix":"10.1159","volume":"15","author":[{"given":"Xuan","family":"Chen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiang","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"QingQiang","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhen","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gang","family":"Liao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"ZiWei","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"127","published-online":{"date-parts":[[2022,3,17]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1742-4658.2007.05639.x"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1038\/nature07203"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1007\/s11695-013-1030-z"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1097\/01.sla.0000102989.54824.fc"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1007\/s002680020348"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1097\/00000658-200211000-00003"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2004-0433"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1016\/j.soard.2016.08.500"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.3892\/etm.2014.1832"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1155\/2008\/704045"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1381\/0960892053723367"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.2337\/db11-0203"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1097\/SLA.0b013e31826b8603"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1210\/endo.143.6.8840"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2011.09.061"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1038\/s41387-018-0062-9"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.2337\/db13-0856"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1016\/j.soard.2014.04.019"}],"container-title":["Obesity Facts"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/519417","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/519417","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T15:25:28Z","timestamp":1745421928000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000519417"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022]]},"references-count":18,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2022,5,17]]}},"URL":"https:\/\/doi.org\/10.1159\/000519417","archive":["Portico"],"relation":{},"ISSN":["1662-4025","1662-4033"],"issn-type":[{"value":"1662-4025","type":"print"},{"value":"1662-4033","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022]]}}}