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Karger AG","issue":"4","license":[{"start":{"date-parts":[[2021,12,14]],"date-time":"2021-12-14T00:00:00Z","timestamp":1639440000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"},{"start":{"date-parts":[[2021,12,14]],"date-time":"2021-12-14T00:00:00Z","timestamp":1639440000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sex Dev"],"published-print":{"date-parts":[[2022]]},"abstract":"<jats:p>The human chorionic gonadotropin (hCG) stimulation test that evaluates gonadal steroidogenesis is crucial in the assessment of patients with 46,XY disorders of sex development (DSD). This study aimed to determine a testosterone (T) cutoff level that indicates an adequate testicular function using LC-MS\/MS after stimulation with recombinant human chorionic gonadotropin (rhCG) in a single dose. Nineteen prepubertal children with 46,XY DSD and normal T secretion were evaluated. T and dihydrotestosterone (DHT) levels were measured by liquid chromatography technique with tandem mass spectrometry (LC-MS\/MS) before and 7 days after rhCG application at 250 \u00b5g. We suggest 0.89 ng\/mL as the cutoff point for T after rhCG stimulation analyzed by LC-MS\/MS. <\/jats:p>","DOI":"10.1159\/000519422","type":"journal-article","created":{"date-parts":[[2021,12,14]],"date-time":"2021-12-14T22:00:41Z","timestamp":1639519241000},"page":"266-269","source":"Crossref","is-referenced-by-count":5,"title":["Suggested Cutoff Point for Testosterone by Liquid Chromatography with Tandem Mass Spectrometry (LC-MS\/MS) after Stimulation with Recombinant Human Chorionic Gonadotropin"],"prefix":"10.1159","volume":"16","author":[{"given":"Leticia R.","family":"de Oliveira","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6361-5235","authenticated-orcid":false,"given":"Carlos A.","family":"Longui","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7764-1426","authenticated-orcid":false,"given":"Guilherme","family":"Guaragna-Filho","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9 L.","family":"da Costa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rafael","family":"Lanaro","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria I.","family":"Chiamolera","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0521-9100","authenticated-orcid":false,"given":"Maricilda P.","family":"de Mello","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2088-972X","authenticated-orcid":false,"given":"Andr\u00e9 M.","family":"Morcillo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrea T.","family":"Maciel-Guerra","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gil","family":"Guerra-Junior","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2021,12,14]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1159\/000481552"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1159\/000481552"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1016\/j.cca.2017.03.010"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2013-2918"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1159\/000486329"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.4274\/jcrpe.v2i1.1"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1016\/j.jsbmb.2016.05.020"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1016\/j.jsbmb.2010.05.001"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1159\/000313372"}],"container-title":["Sexual Development"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/karger.com\/sxd\/article-pdf\/16\/4\/266\/3914861\/000519422.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/karger.com\/sxd\/article-pdf\/16\/4\/266\/3914861\/000519422.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T15:25:30Z","timestamp":1745421930000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000519422"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,12,14]]},"references-count":9,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2023,2,10]]},"published-print":{"date-parts":[[2023,2,14]]}},"URL":"https:\/\/doi.org\/10.1159\/000519422","archive":["Portico"],"relation":{"is-supplemented-by":[{"id-type":"doi","id":"10.6084\/m9.figshare.17197250","asserted-by":"subject"}]},"ISSN":["1661-5425","1661-5433"],"issn-type":[{"value":"1661-5425","type":"print"},{"value":"1661-5433","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,12,14]]}}}