{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,25]],"date-time":"2026-01-25T00:15:39Z","timestamp":1769300139181,"version":"3.49.0"},"reference-count":17,"publisher":"S. Karger AG","issue":"1-2","license":[{"start":{"date-parts":[[2021,11,15]],"date-time":"2021-11-15T00:00:00Z","timestamp":1636934400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2021,11,15]],"date-time":"2021-11-15T00:00:00Z","timestamp":1636934400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pharmacology"],"published-print":{"date-parts":[[2022]]},"abstract":"Fenoterol is a \u03b2<sub>2<\/sub>-adrenoceptor (AR)-selective agonist that is commonly used to investigate relaxation responses mediated by \u03b2<sub>2<\/sub>-AR in smooth muscle preparations. Some data have questioned this because fenoterol had low potency in the rat urinary bladder when a muscarinic agonist was used as a pre-contraction agent and because some investigators proposed that fenoterol may act in part via \u03b2<sub>3<\/sub>-AR. We designed the present study to investigate whether fenoterol is a proper pharmacological tool to study \u03b2<sub>2<\/sub>-AR-mediated relaxation responses in the rat urinary bladder. Firstly, we have compared the effect of pre-contraction agents on fenoterol potency and found that fenoterol potency was about 1.5 log units greater against KCl than carbachol (pEC<sub>50<\/sub> 7.19 \u00b1 0.66 and 5.62 \u00b1 1.09 of KCl and of carbachol, respectively). To test the selectivity of fenoterol, we have determined the effects of the \u03b2<sub>2<\/sub>-AR antagonist ICI 118,551 and the \u03b2<sub>3<\/sub>-AR antagonist L 748,337 on relaxation responses to fenoterol. While 300 nM L 748,337 had little effect on the potency of fenoterol (pEC<sub>50<\/sub> 6.56 \u00b1 0.25 and 6.33 \u00b1 0.61 in the absence and presence of L 748,337, respectively), the relaxation curve for fenoterol was right-shifted in the presence 300 nM ICI 118,551 (pEC<sub>50<\/sub> 5.03 \u00b1 0.18). Thus, we conclude that fenoterol is a proper pharmacological tool to assess \u03b2<sub>2<\/sub>-AR-mediated responses in the rat urinary bladder and most likely in other smooth-muscle preparations containing multiple subtypes of the \u03b2-AR. <\/jats:p>","DOI":"10.1159\/000519720","type":"journal-article","created":{"date-parts":[[2022,1,6]],"date-time":"2022-01-06T22:03:08Z","timestamp":1641506588000},"page":"116-121","source":"Crossref","is-referenced-by-count":7,"title":["Validation of Fenoterol to Study \u03b2<sub>2<\/sub>-Adrenoceptor Function in the Rat Urinary Bladder"],"prefix":"10.1159","volume":"107","author":[{"given":"Bet\u00fcl Rabia","family":"Erdogan","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zeynep Elif","family":"Yesilyurt","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ebru","family":"Arioglu-Inan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martin Christian","family":"Michel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2021,11,15]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1517\/14740338.2016.1160055"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1111\/bph.14635"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0706619"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1007\/s00210-003-0860-y"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.2010.00754.x"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1007\/s00210-013-0936-2"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2019.00911"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1016\/j.coph.2014.03.003"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1540\/jsmr.38.145"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.2010.00872.x"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1007\/s00345-009-0416-y"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1124\/mol.119.118927"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1038\/d41586-019-00857-9"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1016\/j.urology.2011.08.080"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.2337\/diab.40.9.1150"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1097\/01.ju.0000093561.95283.df"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0706048"}],"container-title":["Pharmacology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/519720","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/519720","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T15:27:50Z","timestamp":1745422070000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000519720"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,11,15]]},"references-count":17,"journal-issue":{"issue":"1-2","published-online":{"date-parts":[[2022,1,6]]},"published-print":{"date-parts":[[2022,1,11]]}},"URL":"https:\/\/doi.org\/10.1159\/000519720","archive":["Portico"],"relation":{"is-supplemented-by":[{"id-type":"doi","id":"10.6084\/m9.figshare.17012447","asserted-by":"subject"}]},"ISSN":["0031-7012","1423-0313"],"issn-type":[{"value":"0031-7012","type":"print"},{"value":"1423-0313","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,11,15]]}}}