{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,6,17]],"date-time":"2025-06-17T09:09:46Z","timestamp":1750151386659,"version":"3.40.5"},"reference-count":19,"publisher":"S. Karger AG","issue":"11-12","license":[{"start":{"date-parts":[[2021,1,1]],"date-time":"2021-01-01T00:00:00Z","timestamp":1609459200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2021,1,1]],"date-time":"2021-01-01T00:00:00Z","timestamp":1609459200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Horm Res Paediatr"],"published-print":{"date-parts":[[2021]]},"abstract":"<b><i>Objective:<\/i><\/b> The aim of this study is to determine the cutoff values of gonadotropin response to gonadotropin-releasing hormone analogs (GnRHas) corresponding to the activation of the hypothalamic-pituitary-gonadal axis that could differentiate central precocious puberty (CPP) from premature thelarche (PT) and using the electrochemiluminescence assay method. <b><i>Methods:<\/i><\/b> A total of 49 girls underwent the stimulation test with an intramuscular injection of 3.75 mg leuprolide acetate. Based on the clinical and laboratory characteristics, they were divided into two groups: CPP (<i>n<\/i> = 22) and PT (<i>n<\/i> = 27). Baseline estradiol, luteinizing hormone (LH), and follicle-stimulating hormone (FSH) were collected before GnRHa administration and LH and FSH at 60 and 120 min, respectively, after GnRHa administration. <b><i>Results:<\/i><\/b> The girls with CPP presented an increased height <i>Z<\/i>-score, advanced bone age, and higher baseline LH, FSH, estradiol, and LH\/FSH ratio in relation to PT (<i>p<\/i> &#x3c; 0.001). Stimulated LH differed significantly between the two groups, and the LH cutoff values were \u22654.29 IU\/L (<i>p<\/i> &#x3c; 0.001) and \u22653.95 IU\/L at 60 and 120 min, respectively (<i>p<\/i> &#x3c; 0.001). The LH peak was found at 60 min after stimulation. <b><i>Conclusions:<\/i><\/b> The GnRHa test is effective in distinguishing CPP from PT, and a single sampling, at 60 min, with LH concentrations above 4.29 may be the parameter of choice with the advantage of greater convenience and practicality. <\/jats:p>","DOI":"10.1159\/000521593","type":"journal-article","created":{"date-parts":[[2021,12,21]],"date-time":"2021-12-21T22:00:36Z","timestamp":1640124036000},"page":"433-440","source":"Crossref","is-referenced-by-count":3,"title":["Assessment of Gonadotropin Concentrations Stimulated by Gonadotropin-Releasing Hormone Analog by Electrochemiluminescence in Girls with Precocious Puberty and Premature Thelarche"],"prefix":"10.1159","volume":"94","author":[{"given":"Kamila Botelho Fernandes","family":"de Souza","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Melyna Shayanne Pess\u00f4a","family":"Veiga","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gabriela R\u00e1ina Ferreira","family":"Martins","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adriana Paula","family":"da Silva","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L\u00edvia Grimaldi Abud","family":"Fujita","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2395-6895","authenticated-orcid":false,"given":"Jana\u00edne Machado","family":"Tom\u00e9","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Helo\u00edsa Marcelina da Cunha","family":"Palhares","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria de F\u00e1tima","family":"Borges","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2021,12,21]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1080\/17446651.2019.1575726"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1016\/S2213-8587(15)00380-0"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1530\/EJE-20-0103"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMcp0800459"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1542\/peds.2015-3732"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1007\/s40272-015-0130-8"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.3389\/fped.2019.00147"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpag.2019.05.011"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2006-1569"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2003-031537"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2265.2010.03796.x"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2265.2011.04185.x"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1186\/1687-9856-2012-23"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1136\/adc.44.235.291"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.2307\/2531595"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2007-1216"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1111\/cen.14124"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1016\/s0022-3476(95)70255-5"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.4103\/2230-8210.95682"}],"container-title":["Hormone Research in Paediatrics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/521593","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/521593","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T15:41:42Z","timestamp":1745422902000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000521593"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021]]},"references-count":19,"journal-issue":{"issue":"11-12","published-online":{"date-parts":[[2022,3,4]]},"published-print":{"date-parts":[[2022,5,20]]}},"URL":"https:\/\/doi.org\/10.1159\/000521593","archive":["Portico"],"relation":{},"ISSN":["1663-2818","1663-2826"],"issn-type":[{"type":"print","value":"1663-2818"},{"type":"electronic","value":"1663-2826"}],"subject":[],"published":{"date-parts":[[2021]]}}}