{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T04:26:03Z","timestamp":1745468763859,"version":"3.40.4"},"reference-count":15,"publisher":"S. Karger AG","issue":"3","license":[{"start":{"date-parts":[[2022,1,1]],"date-time":"2022-01-01T00:00:00Z","timestamp":1640995200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2022,1,1]],"date-time":"2022-01-01T00:00:00Z","timestamp":1640995200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Neonatology"],"published-print":{"date-parts":[[2022]]},"abstract":"<b><i>Introduction:<\/i><\/b> Striatal neurons of term newborns are highly vulnerable to hypoxia-ischemia (H-I). In a piglet model of H-I, a dopamine D1 receptor antagonist and an adenosine A<sub>2A<\/sub> receptor antagonist alone preferentially protect striatonigral and striatopallidal neurons, respectively. Here, we tested the hypothesis whether the combined treatment with SCH23390, a D1 receptor antagonist, and SCH58261, an A<sub>2A<\/sub> receptor antagonist, is more efficacious than individual D1 and A<sub>2A<\/sub> receptor antagonist treatment. <b><i>Methods:<\/i><\/b> Anesthetized newborn piglets were subjected to sham operation (<i>n<\/i> = 6) or 40 min of hypoxia and 7 min of airway occlusion. At 5 min of reoxygenation, piglets received the vehicle, SCH23390, SCH58261, or the combined treatment (<i>n<\/i> = 9 in each group). At 4 days of recovery, the number of viable neurons in the entire putamen was estimated by unbiased stereology. <b><i>Results:<\/i><\/b> Stereological results showed that sham-operated piglets had an estimated 2.9 \u00d7 10<sup>6<\/sup> neurons in the putamen, and the number of viable neurons in hypoxic-ischemic piglets was significantly reduced by 80% to 0.6 \u00d7 10<sup>6<\/sup>\/putamen. Treatment with SCH23390, SCH58261, and the combination increased the numbers of viable neurons to 1.4 \u00d7 10<sup>6<\/sup>\/putamen, 1.4 \u00d7 10<sup>6<\/sup>\/putamen, and 2.1 \u00d7 10<sup>6<\/sup>\/putamen, respectively. Notably, the combined treatment improved neuroprotection compared to individual therapy. <b><i>Conclusion:<\/i><\/b> We conclude that simultaneous inhibition of dopamine D1 receptors and adenosine A<sub>2A<\/sub> receptors saves more neurons than individual treatment in the highly vulnerable putamen of a large-animal neonatal H-I model. <\/jats:p>","DOI":"10.1159\/000524207","type":"journal-article","created":{"date-parts":[[2022,4,27]],"date-time":"2022-04-27T21:00:36Z","timestamp":1651093236000},"page":"354-360","source":"Crossref","is-referenced-by-count":2,"title":["Neuroprotection in the Striatum of Hypoxic-Ischemic Piglets by Simultaneous Inhibition of Dopamine D1 and Adenosine A<sub>2A<\/sub> Receptors"],"prefix":"10.1159","volume":"119","author":[{"given":"Bing","family":"Wang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ewa","family":"Kulikowicz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Raymond C.","family":"Koehler","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zeng-Jin","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2022,4,27]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1750-3639.2002.tb00467.x"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0800946105"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.neuro.051508.135422"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1126\/science.294.5544.1024"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1046\/j.1471-4159.1995.64010292.x"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1038\/jcbfm.1987.41"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1177\/0271678X18797328"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1002\/ana.410440202"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1177\/088307389801300702"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1002\/(sici)1096-9861(19970113)377:2%3c262::aid-cne8%3e3.0.co;2-1"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1523\/jneurosci.13-08-03510.1993"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1002\/(sici)1096-9861(20000228)418:1%3c22::aid-cne2%3e3.0.co;2-q"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1124\/pr.55.3.2"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1016\/s0028-3908(03)00250-8"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.3389\/fped.2019.00211"}],"container-title":["Neonatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/524207","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/524207","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T15:59:26Z","timestamp":1745423966000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000524207"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022]]},"references-count":15,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2022,5,17]]},"published-print":{"date-parts":[[2022,5,24]]}},"URL":"https:\/\/doi.org\/10.1159\/000524207","archive":["Portico"],"relation":{"is-supplemented-by":[{"id-type":"doi","id":"10.6084\/m9.figshare.19664283","asserted-by":"subject"}]},"ISSN":["1661-7800","1661-7819"],"issn-type":[{"type":"print","value":"1661-7800"},{"type":"electronic","value":"1661-7819"}],"subject":[],"published":{"date-parts":[[2022]]}}}