{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T03:31:24Z","timestamp":1747193484998,"version":"3.40.5"},"reference-count":4,"publisher":"S. Karger AG","issue":"5","license":[{"start":{"date-parts":[[2022,1,1]],"date-time":"2022-01-01T00:00:00Z","timestamp":1640995200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2022,1,1]],"date-time":"2022-01-01T00:00:00Z","timestamp":1640995200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Acta Haematol"],"published-print":{"date-parts":[[2022]]},"abstract":"<jats:p>Through an RNA-seq analysis of an adult patient with unclassifiable myelodysplastic\/myeloproliferative neoplasms (MDS\/MPN-U), we identified a rare &lt;i&gt;PDGFRB&lt;\/i&gt; fusion partner gene, &lt;i&gt;PCM1&lt;\/i&gt;. Conventional chromosome karyotype analysis showed abnormal clones of t(5;8)(q32;p22), and fluorescence in situ hybridization (FISH) confirmed rearrangement of the &lt;i&gt;PDGFRB&lt;\/i&gt; gene. Reverse transcription PCR (RT-PCR) and Sanger sequencing further confirmed that exon 30 of the &lt;i&gt;PCM1&lt;\/i&gt; gene was fused with exon 11 of &lt;i&gt;PDGFRB&lt;\/i&gt; in frame, and the fusion event was accompanied by a 14 bp deletion of exon 11 of &lt;i&gt;PDGFRB&lt;\/i&gt;. After low-dose imatinib treatment, the patient achieved complete molecular remission. This study not only broadens the understanding of myeloid\/lymphoid neoplasms with &lt;i&gt;PDGFRB&lt;\/i&gt; rearrangement but also reflects the vital role of RNA-seq in identifying &lt;i&gt;PDGFRB&lt;\/i&gt; rearrangements. <\/jats:p>","DOI":"10.1159\/000524275","type":"journal-article","created":{"date-parts":[[2022,3,28]],"date-time":"2022-03-28T06:50:03Z","timestamp":1648450203000},"page":"560-565","source":"Crossref","is-referenced-by-count":2,"title":["Myeloid Neoplasm with &lt;b&gt;&lt;i&gt;PCM1-PDGFRB&lt;\/i&gt;&lt;\/b&gt; Transcript Responded to Low-Dose Imatinib: One Case Report with Literature Review"],"prefix":"10.1159","volume":"145","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9129-4125","authenticated-orcid":false,"given":"Zhe","family":"Wang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li","family":"Wan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dong","family":"Lin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cheng-Wen","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zheng","family":"Tian","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ying-Chang","family":"Mi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2022,3,25]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.4065\/81.9.1241"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1002\/ajh.25617"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-004-4272-z"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1101\/gad.1653708"}],"container-title":["Acta Haematologica"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/524275","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/524275","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T16:00:24Z","timestamp":1745424024000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000524275"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022]]},"references-count":4,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2022,9,20]]}},"URL":"https:\/\/doi.org\/10.1159\/000524275","archive":["Portico"],"relation":{"is-supplemented-by":[{"id-type":"doi","id":"10.6084\/m9.figshare.19419320","asserted-by":"subject"}]},"ISSN":["0001-5792","1421-9662"],"issn-type":[{"type":"print","value":"0001-5792"},{"type":"electronic","value":"1421-9662"}],"subject":[],"published":{"date-parts":[[2022]]}}}