{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T15:33:04Z","timestamp":1784302384622,"version":"3.55.0"},"reference-count":16,"publisher":"S. Karger AG","issue":"5","license":[{"start":{"date-parts":[[2022,1,1]],"date-time":"2022-01-01T00:00:00Z","timestamp":1640995200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2022,1,1]],"date-time":"2022-01-01T00:00:00Z","timestamp":1640995200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["ORL"],"published-print":{"date-parts":[[2022]]},"abstract":"<b><i>Introduction:<\/i><\/b> CD8<sup>+<\/sup>CD25<sup>+<\/sup>Foxp3<sup>+<\/sup> regulatory T cells (Tregs) play an important role in human\u2019s immune tolerance. The study was aimed to assess the influence of budesonide nasal spray on CD8<sup>+<\/sup>CD25<sup>+<\/sup>Foxp3<sup>+<\/sup> Tregs and to evaluate their cellular functions in neutrophilic chronic rhinosinusitis with nasal polyps (CRSwNPs). <b><i>Methods:<\/i><\/b> Fifteen patients with neutrophilic CRSwNPs were enrolled and received physiological saline or budesonide nasal spray treatment (Saline or Budesonide group) for 3 months. Nasal tissue samples were obtained from normal subjects or those patients and cultured in vitro. CD8<sup>+<\/sup>CD25<sup>+<\/sup>Foxp3<sup>+<\/sup> Tregs were separated from normal or NP tissues and also cultured in vitro. Then interleukin (IL)-10 and its mRNA were evaluated in the above cell cultures. The cells were applied into NP cultures. Finally, myeloperoxidase (MPO), interferon (IFN)-\u03b3, IL-1\u03b2, and tumor necrosis factor (TNF)-\u03b1 were assessed in the tissue cultures. <b><i>Results:<\/i><\/b> CD8<sup>+<\/sup>CD25<sup>+<\/sup>Foxp3<sup>+<\/sup> Tregs decreased in NP tissues. Budesonide administration did not enhance the percentage of these cells in polypoid tissues. IL-10 and its mRNA were increased in the above cell cultures from NPs. However, there were no statistical differences between the two treatments in the IL-10 expression. Additionally, levels of MPO, IFN-\u03b3, IL-1\u03b2, and TNF-\u03b1 were totally elevated in NP tissue cultures and reduced after the administration of CD8<sup>+<\/sup>CD25<sup>+<\/sup>Foxp3<sup>+<\/sup> Tregs. However, there were no significant differences in concentrations of these mediators between these two groups of the CD8<sup>+<\/sup>CD25<sup>+<\/sup>Foxp3<sup>+<\/sup> Tregs treatment in vitro. <b><i>Conclusion:<\/i><\/b> The findings indicate that CD8<sup>+<\/sup>CD25<sup>+<\/sup>Foxp3<sup>+<\/sup> Tregs might regulate the neutrophilic inflammation, and budesonide nasal spray therapy could not ameliorate the inflammation in neutrophilic CRSwNPs. <\/jats:p>","DOI":"10.1159\/000524385","type":"journal-article","created":{"date-parts":[[2022,4,25]],"date-time":"2022-04-25T21:00:47Z","timestamp":1650920447000},"page":"396-405","source":"Crossref","is-referenced-by-count":2,"title":["Effect of Local Corticosteroid Administration on CD8<sup>+<\/sup>CD25<sup>+<\/sup>Foxp3<sup>+<\/sup> Tregs in Neutrophilic CRSwNP"],"prefix":"10.1159","volume":"84","author":[{"given":"Lin","family":"Lin","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Lan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fei","family":"Dai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinjin","family":"Wei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zheng","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"127","published-online":{"date-parts":[[2022,4,25]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1002\/lary.21969"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.2500\/108854196778662264"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.2500\/ajra.2014.28.4003"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1038\/ni.1818"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1172\/jci23961"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.7326\/0003-4819-154-5-201103010-00003"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1159\/000507395"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.2500\/ajr.2007.21.3050"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1177\/0194599813495363"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1016\/j.anl.2014.12.004"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1016\/s0385-8146(03)00051-8"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1136\/thx.2009.126722"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1053\/rmed.1999.0756"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2004-03-1214"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-065x.2001.1820109.x"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.2500\/ajra.2013.28.4014"}],"container-title":["ORL"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/524385","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/524385","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T16:01:47Z","timestamp":1745424107000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000524385"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022]]},"references-count":16,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2022,9,20]]},"published-print":{"date-parts":[[2022,9,27]]}},"URL":"https:\/\/doi.org\/10.1159\/000524385","archive":["Portico"],"relation":{},"ISSN":["0301-1569","1423-0275"],"issn-type":[{"value":"0301-1569","type":"print"},{"value":"1423-0275","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022]]}}}