{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T03:32:18Z","timestamp":1747193538040,"version":"3.40.5"},"reference-count":17,"publisher":"S. Karger AG","issue":"5","license":[{"start":{"date-parts":[[2022,1,1]],"date-time":"2022-01-01T00:00:00Z","timestamp":1640995200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2022,1,1]],"date-time":"2022-01-01T00:00:00Z","timestamp":1640995200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Vasc Res"],"published-print":{"date-parts":[[2022]]},"abstract":"<b><i>Introduction:<\/i><\/b> Adrenoceptor and endothelin (ET) receptor-mediated vasoconstriction as well as endothelium-dependent vasodilation of human saphenous veins were compared before and after 20 h of cold storage. <b><i>Methods:<\/i><\/b> Contractile responses to potassium chloride (KCl), norepinephrine (NE), and ET-1 as well as vasodilator responses to acetylcholine (ACh) were evaluated. <b><i>Results:<\/i><\/b> Storage in HEPES-supplemented Dulbecco\u2019s modified Eagle\u2019s medium (HDMEM) diminished KCl induced contractile forces to 71% (<i>p<\/i> = 0.002) and NE induced contractions to 80% (<i>p<\/i> = 0.037), in contrast to HEPES-supplemented Krebs-Henseleit solution (HKH) and TiProtec solution. KCl-normalized NE contractions were not affected by storage. NE EC<sub>50<\/sub> values were slightly lower (7.1E\u22128 vs. 7.5E\u22128, <i>p<\/i> = 0.019) after storage in HKH, with no changes after storage in the other solutions. Endothelium-dependent responses to ACh were not affected by storage. ET-1 induced contractions were attenuated after storage in HDMEM (77%, <i>p<\/i> = 0.002), HKH (75%, <i>p<\/i> = 0.020), and TiProtec (73%, <i>p<\/i> = 0.010) with no changes in normalized constrictions. ET-1 EC<sub>50<\/sub> values were not affected by storage. <b><i>Conclusion:<\/i><\/b> Loss of contractility after storage in HDMEM may reflect the lower content of dextrose. There was no specific attenuation of adrenoceptor, ET-receptor, or ACh receptor mediated signal transduction after storage in any of the media. 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