{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T01:33:05Z","timestamp":1788571985150,"version":"build-2803163510"},"reference-count":19,"publisher":"S. Karger AG","issue":"2","license":[{"start":{"date-parts":[[2022,6,21]],"date-time":"2022-06-21T00:00:00Z","timestamp":1655769600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"},{"start":{"date-parts":[[2022,6,21]],"date-time":"2022-06-21T00:00:00Z","timestamp":1655769600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nephron"],"published-print":{"date-parts":[[2023]]},"abstract":"<jats:p>Introduction: Focal segmental glomerulosclerosis (FSGS) is characterized by focal and segmental obliteration of glomerular capillary tufts with increased matrix and usually associated with nephrotic range proteinuria. FSGS is a huge burden to society; however, the mechanisms remain unclear and treatment is still lacking. Methods: Adriamycin nephropathy was induced by Adriamycin injection and some mice were also injected with Anti-miR-155-5p LNA or YC-1 (a pharmacological inhibitor of HIF-1). At 6 weeks, the mice were sacrificed, and kidneys, blood and urine samples were collected for further analysis. Results: We demonstrated a significant increase of miR-155-5p in kidney tissues in Adriamycin-induced FSGS mouse models. We also found Adriamycin treatment led to the activation of HIF-1, and inhibition of HIF-1 using YC-1 partly prevented the induction of miR-155-5p. Functionally, anti-miR-155-5p attenuated kidney injury and delayed the progression of renal fibrosis. Further, anti-miR-155-5p also enhanced the expression of Nrf2 following Adriamycin treatment. Further, our luciferase microRNA target reporter assay verified Nrf2 as a direct target of miR-155-5p. Our further results indicated anti-miR-155-5p could suppress kidney oxidative stress and inflammation, also supporting Nrf2 was the direct target of miR-155-5p. Finally, we also found miR-155-5p did not increase in serum but significantly increased in urine, indicating urinary miR-155-5p may be useful for FSGS diagnosis. Conclusion: This study identified a HIF-1\/miR-155-5p\/Nrf2 axis which can promote kidney oxidative stress and inflammation, finally aggravating kidney injury and accelerating the progression of renal fibrosis in FSGS. Moreover, the increase in urinary miR-155-5p may be useful for the diagnosis of FSGS. <\/jats:p>","DOI":"10.1159\/000525233","type":"journal-article","created":{"date-parts":[[2022,6,21]],"date-time":"2022-06-21T21:00:36Z","timestamp":1655845236000},"page":"108-119","source":"Crossref","is-referenced-by-count":14,"title":["MicroRNA-155-5p Aggravates Adriamycin-Induced Focal Segmental Glomerulosclerosis through Targeting Nrf2"],"prefix":"10.1159","volume":"147","author":[{"given":"Guoyong","family":"Liu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liyu","family":"He","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaomeng","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lingling","family":"Tang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Shi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian","family":"She","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiali","family":"Wei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"127","published-online":{"date-parts":[[2022,6,21]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.2215\/CJN.05960616"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1038\/nrneph.2014.216"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.4132\/jptm.2016.09.21"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1007\/s40620-016-0333-2"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1016\/j.kint.2018.01.020"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1016\/j.ekir.2018.10.016"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1038\/nrneph.2014.202"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00048.2019"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.4103\/ijn.IJN_264_16"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1038\/s41581-018-0103-6"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2020.114301"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.3109\/0886022X.2016.1165119"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1159\/000497821"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1038\/ki.2012.439"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.3892\/br.2020.1393"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1155\/2016\/4632768"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1177\/1535370220902696"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1155\/2020\/6263921"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2013.07.025"}],"container-title":["Nephron"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/karger.com\/nef\/article-pdf\/147\/2\/108\/3953236\/000525233.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/karger.com\/nef\/article-pdf\/147\/2\/108\/3953236\/000525233.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T15:02:24Z","timestamp":1745420544000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000525233"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,6,21]]},"references-count":19,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2023,3,20]]},"published-print":{"date-parts":[[2023,3,21]]}},"URL":"https:\/\/doi.org\/10.1159\/000525233","archive":["Portico"],"relation":{},"ISSN":["1660-8151","2235-3186"],"issn-type":[{"value":"1660-8151","type":"print"},{"value":"2235-3186","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,6,21]]}}}