{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,19]],"date-time":"2026-06-19T23:50:30Z","timestamp":1781913030680,"version":"3.54.5"},"reference-count":37,"publisher":"S. Karger AG","issue":"3","license":[{"start":{"date-parts":[[2022,11,24]],"date-time":"2022-11-24T00:00:00Z","timestamp":1669248000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"},{"start":{"date-parts":[[2022,11,24]],"date-time":"2022-11-24T00:00:00Z","timestamp":1669248000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathobiology"],"published-print":{"date-parts":[[2023]]},"abstract":"<jats:p>Introduction: Angioimmunoblastic T-cell lymphoma (AITL) is an aggressive T-cell lymphoma commonly associated with B-cell dysregulation. Correlations involving B-cell dysregulation and clinicopathological features remain unclear. Methods: We prospectively collected blood samples from 11 AITL patients and 17 healthy controls. The percentages of B-cell subpopulations and lymphocytes with IL-21 production were assessed using flow cytometry. Peripheral blood lymphocyte morphology was evaluated microscopically. Results: Six of 11 (54.5%) patients presented with polyclonal hypergammaglobulinemia. Three of 11 (27.3%) tumor biopsies showed monoclonal immunoglobulin gene rearrangement. The patients exhibited significantly lower levels of naive (p &lt; 0.001) and class-switched (p &lt; 0.001) B cells than controls. The percentages of IgD\u2212CD27\u2212 B cells (p = 0.007) and antibody-secreting cells (ASCs) (p = 0.001) were increased. Blood smears revealed atypical lymphocytes and immature plasma cells with morphological diversity. In comparison to normal controls, IL-21 production significantly increased in CD4+ (p &lt; 0.001) and CD8+ (p = 0.020) T cells. B-cell clonality, RHOA G17V mutation, and the presence of sheets of clear cells and immature\/mature plasma cells in lymph nodes were significantly associated with percentages of class-switched B cells and ASCs. The patients with circulating EBV DNA had a lower percentage of naive B cells (p = 0.009). Conclusions: Our results demonstrated a wide spectrum of peripheral B-cell morphologies and immunophenotypes of peripheral B cells in AITL. These findings correspond to dysregulated B-cell immunity and heterogeneous clinicopathological features. <\/jats:p>","DOI":"10.1159\/000526284","type":"journal-article","created":{"date-parts":[[2022,11,24]],"date-time":"2022-11-24T22:28:01Z","timestamp":1669328881000},"page":"187-198","source":"Crossref","is-referenced-by-count":3,"title":["Flow Cytometry Analysis Reveals a Wide Cytologic and Immunophenotypic Spectrum of Peripheral B Lymphocytes in Angioimmunoblastic T-Cell Lymphoma"],"prefix":"10.1159","volume":"90","author":[{"given":"Hung-Lin","family":"Liu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chun-Kai","family":"Liao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shao-Wen","family":"Weng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lee-Yung","family":"Shih","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chih-Chi","family":"Chou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huey-Ling","family":"You","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming-Chung","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6442-3713","authenticated-orcid":false,"given":"Wan-Ting","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"127","published-online":{"date-parts":[[2022,11,24]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2015.126300"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1038\/s41375-020-0990-y"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2011.37.3647"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2017-11-818617"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.175.12.7867"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.3390\/cancers13040610"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1093\/ajcp\/aqab090"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1084\/jem.194.7.927"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.2007.06620.x"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2003-09-3080"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1016\/j.humpath.2018.05.026"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmoldx.2021.07.008"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexmp.2019.104294"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1097\/PAS.0000000000000651"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1182\/blood.v99.2.627"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.21162"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1007\/s10875-022-01210-y"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2020.606338"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1111\/cei.13615"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcancer.2013.11.004"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1016\/j.it.2016.06.001"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1007\/s12185-013-1411-z"},{"key":"ref23","doi-asserted-by":"publisher","DOI":"10.1007\/s00277-017-3189-1"},{"key":"ref24","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2019.00509"},{"key":"ref25","doi-asserted-by":"publisher","DOI":"10.1097\/PAS.0b013e3180339f18"},{"key":"ref26","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2012.43.3797"},{"key":"ref27","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.165.6.2975"},{"key":"ref28","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.179.5.3153"},{"key":"ref29","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0509311102"},{"key":"ref30","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01780-09"},{"key":"ref31","doi-asserted-by":"publisher","DOI":"10.1084\/jem.190.4.567"},{"key":"ref32","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2014.12.047"},{"key":"ref33","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2013-06-506865"},{"key":"ref34","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2009-06-227579"},{"key":"ref35","doi-asserted-by":"publisher","DOI":"10.1038\/cmi.2017.133"},{"key":"ref36","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2011-06-357996"},{"key":"ref37","doi-asserted-by":"publisher","DOI":"10.1038\/nri3216"}],"container-title":["Pathobiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/karger.com\/pat\/article-pdf\/90\/3\/187\/3962268\/000526284.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/karger.com\/pat\/article-pdf\/90\/3\/187\/3962268\/000526284.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T15:14:21Z","timestamp":1745421261000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000526284"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,11,24]]},"references-count":37,"journal-issue":{"issue":"3","publish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