{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T03:32:10Z","timestamp":1747193530641,"version":"3.40.5"},"reference-count":39,"publisher":"S. Karger AG","issue":"6","license":[{"start":{"date-parts":[[2022,1,1]],"date-time":"2022-01-01T00:00:00Z","timestamp":1640995200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2022,1,1]],"date-time":"2022-01-01T00:00:00Z","timestamp":1640995200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Vasc Res"],"published-print":{"date-parts":[[2022]]},"abstract":"<b><i>Introduction:<\/i><\/b> Beta-aminopropionitrile (BAPN) administration is a chemically induced model for preclinical aortic pathologies research. Angiotensin II (AngII) has been widely used to promotes aortic dissections in mice. Here, we provide insight on a modified aortic dissection model in rats. The effect of smooth muscle cell (SMC) relaxation with vasodilators is studied in this model. <b><i>Methods:<\/i><\/b> Forty Sprague-Dawley rats were divided in 4 groups: control, isosorbide dinitrate (ISDN, 30 mg\/kg\/day) in the drinking water, BAPN (0.02%) in the food, BAPN + ISDN (same doses). Thoracic and abdominal aortic diameters were evaluated through transthoracic ultrasound echography. After 6 weeks, all rats were infused with AngII (1 mg\/kg\/day) subcutaneously. Survival and type of aortic events were numbered. Histological and histochemical analyses of aorta were performed. <b><i>Results:<\/i><\/b> Initial telesystolic ascending aorta diameters were equal in all groups and became significantly larger in the BAPN + ISDN group compared to the BAPN group (control: 3.37 \u00b1 0.17 mm, ISDN: 3.49 \u00b1 0.16 mm, BAPN: 3.53 \u00b1 0.13 mm, BAPN + ISDN: 3.61 \u00b1 0.16 mm, analysis of variance <i>p<\/i> &#x3c; 0.0001). BAPN followed by AngII infusion showed a significant lower survival rate (<i>p<\/i> = 0.029) and produced a large panel of aortic events. Association of ISDN and BAPN significantly reduces survival (<i>p<\/i> = 0.001) and provides more aortic events compared to BAPN alone (<i>p<\/i> = 0.031). In both BAPN-treated groups, orcein staining revealed split and dissected elastic fibers in the media, alcian blue staining showed mucoid degeneration of the aortic wall, and Perls-diaminobenzidine staining revealed an accumulation of Fe<sup>2+<\/sup>. <b><i>Conclusion:<\/i><\/b> SMC relaxation with ISDN increases aortic dilatation, worsens aortic prognosis, and reproduces human histological findings in a low-dose BAPN\/AngII-induced aortic dissection model in rats. <\/jats:p>","DOI":"10.1159\/000526417","type":"journal-article","created":{"date-parts":[[2022,11,10]],"date-time":"2022-11-10T22:00:34Z","timestamp":1668117634000},"page":"369-380","source":"Crossref","is-referenced-by-count":0,"title":["Smooth Muscle Cell Relaxation Worsens Aortic Dilatation and Clinical Presentation in a BAPN\/Angiotensin II-Induced Aortic Dissection Model in Rats"],"prefix":"10.1159","volume":"59","author":[{"given":"Quentin","family":"Pellenc","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sandy","family":"El Bitar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arthur","family":"Darmon","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S\u00e9bastien","family":"Dupont","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jean-Baptiste","family":"Michel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0101-2076","authenticated-orcid":false,"given":"Guillaume","family":"Jondeau","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2022,11,10]]},"reference":[{"key":"ref1"},{"key":"ref2"},{"key":"ref3"},{"key":"ref4"},{"key":"ref5"},{"key":"ref6"},{"key":"ref7"},{"key":"ref8"},{"key":"ref9"},{"key":"ref10"},{"key":"ref11"},{"key":"ref12"},{"key":"ref13"},{"key":"ref14"},{"key":"ref15"},{"key":"ref16"},{"key":"ref17"},{"key":"ref18"},{"key":"ref19"},{"key":"ref20"},{"key":"ref21"},{"key":"ref22"},{"key":"ref23"},{"key":"ref24"},{"key":"ref25"},{"key":"ref26"},{"key":"ref27"},{"key":"ref28"},{"key":"ref29"},{"key":"ref30"},{"key":"ref31"},{"key":"ref32"},{"key":"ref33"},{"key":"ref34"},{"key":"ref35"},{"key":"ref36"},{"key":"ref37"},{"key":"ref38"},{"key":"ref39"}],"container-title":["Journal of Vascular Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/526417","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/526417","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T15:15:44Z","timestamp":1745421344000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000526417"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022]]},"references-count":39,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2022,12,21]]},"published-print":{"date-parts":[[2023,1,10]]}},"URL":"https:\/\/doi.org\/10.1159\/000526417","archive":["Portico"],"relation":{"is-supplemented-by":[{"id-type":"doi","id":"10.6084\/m9.figshare.21532929","asserted-by":"subject"}]},"ISSN":["1018-1172","1423-0135"],"issn-type":[{"type":"print","value":"1018-1172"},{"type":"electronic","value":"1423-0135"}],"subject":[],"published":{"date-parts":[[2022]]}}}