{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,30]],"date-time":"2025-05-30T16:47:41Z","timestamp":1748623661793,"version":"3.40.5"},"reference-count":0,"publisher":"S. Karger AG","issue":"1","license":[{"start":{"date-parts":[[2022,10,14]],"date-time":"2022-10-14T00:00:00Z","timestamp":1665705600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2022,10,14]],"date-time":"2022-10-14T00:00:00Z","timestamp":1665705600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intervirology"],"published-print":{"date-parts":[[2023]]},"abstract":"<jats:p>&lt;b&gt;&lt;i&gt;Introduction:&lt;\/i&gt;&lt;\/b&gt; Most intractable diarrheas are treated with antibiotics, irrespective of the causative agent. This study aimed to quantify rotavirus (RV) with TaqMan real-time PCR in fecal samples of children with acute gastroenteritis (AGE) in accordance with the program of reduction of drug resistance and use of antibiotics. &lt;b&gt;&lt;i&gt;Methods:&lt;\/i&gt;&lt;\/b&gt; A total of 190 fecal specimens were collected from under-5-year-old children with AGE in pediatric Hospital Regina Margherita of Turin in Italy in 2017. A total of 38 out of 67 (56.7%) episodes of AGE were associated with RV genomic detection with a multiplex commercial kit. &lt;b&gt;&lt;i&gt;Results:&lt;\/i&gt;&lt;\/b&gt; All fecal specimens were tested for the presence of RV and other GE viruses. The most commonly detected virus was norovirus (41%), astrovirus (15.8%), human bocavirus (8.9%), sapovirus (7.9%), human parechovirus (5.8%), rhinovirus (4.2%), and adenovirus (1%). In 66 out of 190 (34.7%), RV was detectable with the median viral load 7.2E + 11 \u00b1 60E + 11 genomes\/mg fecal specimens. &lt;b&gt;&lt;i&gt;Discussion\/Conclusions:&lt;\/i&gt;&lt;\/b&gt; Our results showed that RV was present in around 34.7% of children hospitalized for AGE, a rate similar to those reported in previous studies conducted elsewhere which were in the range of 33\u201375%. Our protocols are able to quantify the absolute number of viral particle\/mg of feces. The clinical utility of quantitative molecular assays, such as RV viral load, will be markedly improved. <\/jats:p>","DOI":"10.1159\/000526839","type":"journal-article","created":{"date-parts":[[2022,10,14]],"date-time":"2022-10-14T21:00:26Z","timestamp":1665781226000},"page":"23-29","source":"Crossref","is-referenced-by-count":3,"title":["Rotavirus Quantification in Children with Acute Gastroenteritis"],"prefix":"10.1159","volume":"66","author":[{"given":"Ilaria","family":"Galliano","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Valentina","family":"Dapr\u00e0","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maddalena","family":"Dini","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martina","family":"Gavatorta","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Allegra","family":"Sardo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giada","family":"Lo Curcio","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9291-1332","authenticated-orcid":false,"given":"Massimiliano","family":"Bergallo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2022,10,14]]},"container-title":["Intervirology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/karger.com\/int\/article-pdf\/66\/1\/23\/3962716\/000526839.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/karger.com\/int\/article-pdf\/66\/1\/23\/3962716\/000526839.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T15:19:57Z","timestamp":1745421597000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000526839"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,10,14]]},"references-count":0,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2023,12,20]]}},"URL":"https:\/\/doi.org\/10.1159\/000526839","archive":["Portico"],"relation":{},"ISSN":["0300-5526","1423-0100"],"issn-type":[{"type":"print","value":"0300-5526"},{"type":"electronic","value":"1423-0100"}],"subject":[],"published":{"date-parts":[[2022,10,14]]}}}