{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T13:09:15Z","timestamp":1787836155240,"version":"build-2784847793"},"reference-count":62,"publisher":"S. Karger AG","issue":"9","license":[{"start":{"date-parts":[[2023,1,1]],"date-time":"2023-01-01T00:00:00Z","timestamp":1672531200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2023,1,1]],"date-time":"2023-01-01T00:00:00Z","timestamp":1672531200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int Arch Allergy Immunol"],"published-print":{"date-parts":[[2023]]},"abstract":"<jats:p>Introduction: Neutrophilic granulocytes represent the first line of defense against microorganisms. Granulocytes phagocytose microorganisms and specifically synthesize oxygen radicals against them, which eventually kills the invaders. Methods: Neutrophilic granulocytes were isolated from peripheral blood of healthy volunteer donors. Putative interference of new-generation antibiotics with neutrophil function was tested using a collection of granulocyte-stimulating agents and Amplex\u2122 Red-based plate assay and flow cytometry-based respiratory burst assays. In addition, phagocytosis of E. coli, IL-8 production, bactericidal activity, and CD62L expression of granulocytes were evaluated. Results: Of note, we found that the two glycopeptide antibiotics dalbavancin and teicoplanin inhibited ROS production upon granulocyte activation via different signaling pathways in a dose-dependent manner. Dalbavancin also blocked the PMA-induced shedding of CD62L. In contrast, the oxazolidinone antibiotics tedizolid and linezolid had no effect on neutrophil function, while the combination of ceftazidime\/avibactam dose dependently inhibited the fMLP\/Cytochalasin B-induced granulocyte burst in a dose-dependent manner. Additionally, we showed that dalbavancin and teicoplanin as well as sulfametrole\/trimethoprim and ceftazidime\/avibactam inhibited baseline and PMA-induced IL-8 production by neutrophilic granulocytes. Moreover, dalbavancin impaired the bactericidal activity of neutrophilic granulocytes. Conclusion: We here identified hitherto unknown inhibitory effects of several classes of antibiotics on the effector functions of neutrophilic granulocytes. <\/jats:p>","DOI":"10.1159\/000530865","type":"journal-article","created":{"date-parts":[[2023,6,16]],"date-time":"2023-06-16T21:00:38Z","timestamp":1686949238000},"page":"932-948","source":"Crossref","is-referenced-by-count":4,"title":["Glycopeptide Antibiotics Impair Neutrophil Effector Functions"],"prefix":"10.1159","volume":"184","author":[{"given":"Paul","family":"Ettel","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Al Nasar Ahmed","family":"Sehgal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicole","family":"Harrison","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Winfried F.","family":"Pickl","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katharina","family":"Grabmeier-Pfistershammer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"127","published-online":{"date-parts":[[2023,6,15]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1002\/jlb.56.6.672"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2011.03.028"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1038\/nri3024"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2010.11.011"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1002\/eji.201747053"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1038\/nri3399"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1182\/asheducation-2016.1.43"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1126\/science.162.3859.1277"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1111\/pai.12527"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1080\/13693780802017535"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkn545"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.34.5.863"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1136\/gut.28.2.190"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2036.1999.00484.x"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1016\/s1567-5769(01)00194-1"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.3390\/ijms18122666"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1007\/s00011-016-0999-x"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.02409-18"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1038\/srep07438"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.17.3.322"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1007\/BF02982084"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1007\/s00281-008-0118-3"},{"key":"ref23","doi-asserted-by":"publisher","DOI":"10.1182\/blood.v58.5.975.975"},{"key":"ref24","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.48.3.940-945.2004"},{"key":"ref25","doi-asserted-by":"publisher","DOI":"10.1007\/s40262-017-0601-x"},{"key":"ref26","doi-asserted-by":"publisher","DOI":"10.1007\/s40262-018-0705-y"},{"key":"ref27","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.26.6.881"},{"key":"ref28","doi-asserted-by":"publisher","DOI":"10.1179\/joc.2001.13.3.244"},{"key":"ref29","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics8020054"},{"key":"ref30","doi-asserted-by":"publisher","DOI":"10.1007\/BF00915288"},{"key":"ref31","doi-asserted-by":"publisher","DOI":"10.1172\/JCI109109"},{"key":"ref32","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.23.6.892"},{"key":"ref33","doi-asserted-by":"publisher","DOI":"10.1080\/14656566.2018.1519021"},{"key":"ref34","doi-asserted-by":"publisher","DOI":"10.1248\/bpb.b22-00276"},{"key":"ref35","doi-asserted-by":"publisher","DOI":"10.1016\/j.medmal.2013.09.011"},{"key":"ref36","doi-asserted-by":"publisher","DOI":"10.1055\/s-0034-1396906"},{"key":"ref37","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00228-09"},{"key":"ref38","doi-asserted-by":"publisher","DOI":"10.1159\/000092539"},{"key":"ref39","doi-asserted-by":"publisher","DOI":"10.1007\/s00281-008-0121-8"},{"key":"ref40","doi-asserted-by":"publisher","DOI":"10.1161\/01.cir.101.2.122"},{"key":"ref41","doi-asserted-by":"publisher","DOI":"10.1016\/S0009-9236(99)70054-8"},{"key":"ref42","doi-asserted-by":"publisher","DOI":"10.1016\/0195-6701(86)90008-3"},{"key":"ref43","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20021787"},{"key":"ref44","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-immunol-051116-052433"},{"key":"ref45","doi-asserted-by":"publisher","DOI":"10.1093\/cvr\/cvv159"},{"key":"ref46","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M209202200"},{"key":"ref47","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2121-5-21"},{"key":"ref48","doi-asserted-by":"publisher","DOI":"10.1016\/0147-9571(95)98853-a"},{"key":"ref49","doi-asserted-by":"publisher","DOI":"10.1126\/science.2551036"},{"key":"ref50","doi-asserted-by":"publisher","DOI":"10.1002\/eji.201847710"},{"key":"ref51","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.250340"},{"key":"ref52","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2019.01068"},{"key":"ref53","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2006-02-005827"},{"key":"ref54","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0022560"},{"key":"ref55","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.161.8.4332"},{"key":"ref56","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1002680"},{"key":"ref57","doi-asserted-by":"publisher","DOI":"10.1172\/jci.insight.150862"},{"key":"ref58","doi-asserted-by":"publisher","DOI":"10.1016\/j.cyto.2022.155828"},{"key":"ref59","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.155.3.1428"},{"key":"ref60","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2016.00155"},{"key":"ref61","doi-asserted-by":"publisher","DOI":"10.1155\/2018\/4065362"},{"key":"ref62","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.9.5577-5588.2001"}],"container-title":["International Archives of Allergy and Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/karger.com\/iaa\/article-pdf\/184\/9\/932\/3995684\/000530865.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/karger.com\/iaa\/article-pdf\/184\/9\/932\/3995684\/000530865.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T15:29:00Z","timestamp":1745422140000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000530865"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023]]},"references-count":62,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2023,9,4]]},"published-print":{"date-parts":[[2023,9,18]]}},"URL":"https:\/\/doi.org\/10.1159\/000530865","archive":["Portico"],"relation":{"is-supplemented-by":[{"id-type":"doi","id":"10.6084\/m9.figshare.23309249","asserted-by":"subject"}]},"ISSN":["1018-2438","1423-0097"],"issn-type":[{"value":"1018-2438","type":"print"},{"value":"1423-0097","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023]]}}}