{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T21:34:16Z","timestamp":1780608856631,"version":"3.54.1"},"reference-count":13,"publisher":"S. Karger AG","issue":"5-6","license":[{"start":{"date-parts":[[2023,1,1]],"date-time":"2023-01-01T00:00:00Z","timestamp":1672531200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"},{"start":{"date-parts":[[2023,1,1]],"date-time":"2023-01-01T00:00:00Z","timestamp":1672531200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Caries Res"],"published-print":{"date-parts":[[2023]]},"abstract":"<jats:p>A validated protocol used to determine how much of total fluoride is bioavailable in toothpaste formulations was evaluated if it could be simplified regarding the following parameters: (1) concentration of the slurry (0.25\u20134%), (2) centrifugation speed (1,000\u201312,000 g) and time (1\u201310 min), and (3) incubation time for FPO32\u2212 ion hydrolysis and insoluble-F dissolution (15\u201360 min). Fresh and aged Na2FPO3\/CaCO3 and fresh NaF\/SiO2-based toothpastes were analyzed (n = 9). The improvements were the centrifugation at 1,000 g for 5 min is enough to separate insoluble-F and the time for FPO32\u2212 ion hydrolysis and insoluble-F dissolution can be shortened to 30 min. <\/jats:p>","DOI":"10.1159\/000533154","type":"journal-article","created":{"date-parts":[[2023,8,8]],"date-time":"2023-08-08T06:58:00Z","timestamp":1691477880000},"page":"619-624","source":"Crossref","is-referenced-by-count":2,"title":["Optimization of a Validated Protocol That Determines Bioavailable Fluoride in Toothpastes"],"prefix":"10.1159","volume":"57","author":[{"given":"Luis Fernando Bandeira","family":"Miranda","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cinthia Pereira Machado","family":"Tabchoury","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jaime Aparecido","family":"Cury","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"127","published-online":{"date-parts":[[2023,8,1]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-77920-3"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1159\/000521068"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.3109\/00016357.2011.640287"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1159\/000506439"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1590\/s0103-64402010000500003"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-263X.2012.01226.x"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1016\/0300-5712(89)90129-2"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1159\/000350456"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.21615\/cesodon.5606"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1159\/000490196"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.20396\/bjos.v19i0.8661689"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-80503-x"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1159\/000350479"}],"container-title":["Caries Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/karger.com\/cre\/article-pdf\/57\/5-6\/619\/4062596\/000533154.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/karger.com\/cre\/article-pdf\/57\/5-6\/619\/4062596\/000533154.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T15:33:14Z","timestamp":1745422394000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000533154"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023]]},"references-count":13,"journal-issue":{"issue":"5-6","published-online":{"date-parts":[[2023,12,18]]},"published-print":{"date-parts":[[2023,12,19]]}},"URL":"https:\/\/doi.org\/10.1159\/000533154","archive":["Portico"],"relation":{},"ISSN":["0008-6568","1421-976X"],"issn-type":[{"value":"0008-6568","type":"print"},{"value":"1421-976X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023]]}}}