{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,17]],"date-time":"2026-01-17T05:03:27Z","timestamp":1768626207104,"version":"3.49.0"},"reference-count":32,"publisher":"S. Karger AG","issue":"6","license":[{"start":{"date-parts":[[2023,1,1]],"date-time":"2023-01-01T00:00:00Z","timestamp":1672531200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"},{"start":{"date-parts":[[2023,1,1]],"date-time":"2023-01-01T00:00:00Z","timestamp":1672531200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pharmacology"],"published-print":{"date-parts":[[2023]]},"abstract":"<b><i>Introduction:<\/i><\/b> Obesity during pregnancy can contribute to hypertensive complications through changes in glucose utilization. We investigated the impact of vascular glucose uptake, GLUT4 density, and endothelium on agonist-induced vasoconstriction in the aortas of overweight pregnant rats. <b><i>Methods:<\/i><\/b> Isolated aortic rings with or without endothelium from pregnant or nonpregnant rats fed a standard (SD) or hypercaloric diet (HD) were contracted with phenylephrine or serotonin (10<sup>\u22129<\/sup> to 10<sup>\u22124<\/sup><sc>M<\/sc>) using standard (11 m<sc>m<\/sc>) or without (0 m<sc>m<\/sc>) glucose Krebs solution. GLUT4 density in the aortas was measured using the en face method. <b><i>Results:<\/i><\/b> Aortas from overweight pregnant animals (PHD) showed increased Phe-induced vasoconstriction (<i>p<\/i> &lt; 0.05 vs. pregnant standard diet [PSD]), which was endothelium-independent. The contraction decreased significantly in the absence of glucose. In contrast, vessels from pregnant SD rats maintained their contraction in glucose-free Krebs solution. 5-HT increases PHD aortic contraction only in the absence of glucose. The fetal aortas from PHD mothers showed blunted vasoconstriction. Overweight significantly reduced GLUT4 expression in maternal and fetal aortas (<i>p<\/i> &lt; 0.05 vs. PSD). <b><i>Conclusions:<\/i><\/b> Aortic contractility is independent of glucose uptake during healthy pregnancy. In contrast, overweight pregnancy increases contractility. This increase depends directly on smooth muscle glucose uptake and inversely on GLUT-4 density. The increased contraction observed in the vasculature of overweight mothers was inverted in the fetal aortas. <\/jats:p>","DOI":"10.1159\/000533344","type":"journal-article","created":{"date-parts":[[2023,10,11]],"date-time":"2023-10-11T21:03:05Z","timestamp":1697058185000},"page":"521-529","source":"Crossref","is-referenced-by-count":1,"title":["Impact of Normal and Overweight Pregnancy in GLUT4 and Glucose-Dependent Vascular Contractility"],"prefix":"10.1159","volume":"108","author":[{"given":"Esther","family":"Juarez Cort\u00e9s","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gustavo","family":"L\u00f3pez y L\u00f3pez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eduardo I.","family":"Perez Mu\u00f1oz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Betzabel","family":"Rodriguez Reyes","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Damian A.","family":"Madrigal-Aguilar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rosa A.","family":"Bobadilla-Lugo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2023,9,6]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1111\/nure.12055"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1186\/s12884-017-1567-2"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1542\/peds.2004-1808"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00854.2006"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1161\/01.ATV.0000170137.41079.ab"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.REV119.008351"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.3390\/nu12020549"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00116.2005"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1530\/EJE-09-0031"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1677\/JME-09-0091"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1210\/jcem.84.2.5438"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/133.5.1674S"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacc.2013.03.089"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2003.09.025"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1159\/000505144"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.3791\/55460-"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.3390\/biom5043142"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2006-2083"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1186\/s12933-019-0935-9"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2020.00655"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.2337\/diacare.25.3.560"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00243.2002"},{"key":"ref23","doi-asserted-by":"publisher","DOI":"10.1590\/s0004-27302008000600007"},{"key":"ref24","doi-asserted-by":"publisher","DOI":"10.1161\/01.hyp.10.5.512"},{"key":"ref25","doi-asserted-by":"publisher","DOI":"10.1016\/1056-8719(94)00063-a"},{"key":"ref26","doi-asserted-by":"publisher","DOI":"10.1186\/s13098-016-0201-1"},{"key":"ref27","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2018.02.012"},{"key":"ref28","doi-asserted-by":"publisher","DOI":"10.1016\/0031-6989(85)90067-0"},{"key":"ref29","doi-asserted-by":"publisher","DOI":"10.1038\/aps.2009.160"},{"key":"ref30","doi-asserted-by":"publisher","DOI":"10.1111\/j.1748-1716.1985.tb07560.x"},{"key":"ref31","doi-asserted-by":"publisher","DOI":"10.1159\/000158756"},{"key":"ref32","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2018-02778"}],"container-title":["Pharmacology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/karger.com\/pha\/article-pdf\/doi\/10.1159\/000533344\/3997026\/000533344.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/karger.com\/pha\/article-pdf\/doi\/10.1159\/000533344\/3997026\/000533344.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T15:35:30Z","timestamp":1745422530000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000533344"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023]]},"references-count":32,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2023,11,21]]},"published-print":{"date-parts":[[2023,11,21]]}},"URL":"https:\/\/doi.org\/10.1159\/000533344","archive":["Portico"],"relation":{},"ISSN":["0031-7012","1423-0313"],"issn-type":[{"value":"0031-7012","type":"print"},{"value":"1423-0313","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023]]}}}