{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,18]],"date-time":"2026-10-18T04:31:39Z","timestamp":1792297899177,"version":"4.4.0"},"reference-count":18,"publisher":"S. Karger AG","issue":"6","license":[{"start":{"date-parts":[[2023,1,1]],"date-time":"2023-01-01T00:00:00Z","timestamp":1672531200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2023,1,1]],"date-time":"2023-01-01T00:00:00Z","timestamp":1672531200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Dermatology"],"published-print":{"date-parts":[[2023]]},"abstract":"<b><i>Background:<\/i><\/b> Vitiligo is a common depigmentation skin disease associated with significant psychosocial morbidity and profound effect on the quality of life. The treatment of vitiligo is still a major challenge in the field of dermatology. Currently, topical steroids, calcineurin inhibitors, ultraviolet phototherapy, surgery, and cultured and non-cultured epidermal melanocyte transplantation are used for the treatment of vitiligo. However, the effectiveness of these treatment modalities is limited by the lack of response, long-term treatment periods, high cost, and inevitable adverse effects. <b><i>Objectives:<\/i><\/b> In this study, we aimed to evaluate the efficacy of intraepidermal injection of autologous non-cultured melanocytes and keratinocytes as an alternative therapy for the refractory and stable (RS) vitiligo. <b><i>Methods:<\/i><\/b> The treatment procedure was performed on thirty-nine RS vitiligo patients. The autologous skin grafts obtained from the buttock area and epidermis were separated from dermis using dispase. Single-cell autologous melanocytes and keratinocytes were prepared from the epidermis by trypsin\/ethylene diamine tetra acetic acid and injected at the concentration of 100\u2013400 \u00d7 10<sup>3<\/sup> cells\/cm<sup>2<\/sup>, intra-epidermally to the selected vitiligo lesions. Vitiligo re-pigmentation was monitored employing photography. Photographs were taken prior to and 2, 4, and 6 months after the cell transplantation. Improvement of the skin depigmentation was classified as follows: &lt;25% as minimal response, 26\u201350% as moderate response, 51\u201375% as good response, and finally 76\u2013100% as excellent response. <b><i>Results:<\/i><\/b> Cell infusion appeared to be safe as none of the patients exhibited any adverse effects. At the end of the sixth month follow-up period, of the treated patients, 12.8% demonstrated an excellent response, 36% exhibited a good response, and 51.2% showed a moderate to minimal response to the administered therapy. Obtained significant <i>p<\/i> value for Wilcoxon test over the checkpoints at 2nd, 4th, and 6th month (<i>p<\/i> = 0.03, 0.04, and 0.039, respectively) post-cell transplantation confirmed notable growing trend in the re-pigmentation. <b><i>Conclusion:<\/i><\/b> Our findings provide a strong support for the therapeutic efficacy of autologous non-cultured melanocytes and keratinocytes in patients with RS vitiligo. <\/jats:p>","DOI":"10.1159\/000533353","type":"journal-article","created":{"date-parts":[[2023,8,11]],"date-time":"2023-08-11T21:01:23Z","timestamp":1691787683000},"page":"919-925","source":"Crossref","is-referenced-by-count":9,"title":["Safety and Efficacy of Autologous Melanocyte\/Keratinocyte Transplantation in Patients with Refractory Stable Vitiligo"],"prefix":"10.1159","volume":"239","author":[{"given":"Atefeh","family":"Shahbazi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manuchehr","family":"Abedi Valugerdi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sepide","family":"Kazemi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarvenaz","family":"Samadi Kochaksaraei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammad Hassan","family":"Naseh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nasser","family":"Aghdami","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Behnam","family":"Sadeghi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"127","published-online":{"date-parts":[[2023,8,11]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1002\/14651858.CD003263.pub5"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1155\/2015\/631927"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaad.2016.11.010"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1016\/j.det.2016.11.014"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2021.790125"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaad.2003.12.013"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.2011.10398.x"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaad.2017.01.056"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1111\/pcmr.12600"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1007\/s00403-010-1034-7"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1097\/01.prs.0000280564.25786.bf"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.3109\/09546634.2015.1034068"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1016\/j.clindermatol.2004.07.026"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaad.2011.05.002"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-4632.2005.02226.x"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaad.2010.11.061"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-4632.2004.02403.x"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1001\/archderm.136.11.1380"}],"container-title":["Dermatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/karger.com\/drm\/article-pdf\/239\/6\/919\/4054221\/000533353.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/karger.com\/drm\/article-pdf\/239\/6\/919\/4054221\/000533353.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T15:35:37Z","timestamp":1745422537000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000533353"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023]]},"references-count":18,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2023,12,5]]},"published-print":{"date-parts":[[2023,12,5]]}},"URL":"https:\/\/doi.org\/10.1159\/000533353","archive":["Portico"],"relation":{},"ISSN":["1018-8665","1421-9832"],"issn-type":[{"value":"1018-8665","type":"print"},{"value":"1421-9832","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023]]}}}