{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T20:55:24Z","timestamp":1761598524478,"version":"3.40.4"},"reference-count":32,"publisher":"S. Karger AG","issue":"6","license":[{"start":{"date-parts":[[2023,1,1]],"date-time":"2023-01-01T00:00:00Z","timestamp":1672531200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2023,1,1]],"date-time":"2023-01-01T00:00:00Z","timestamp":1672531200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pharmacology"],"published-print":{"date-parts":[[2023]]},"abstract":"<b><i>Introduction:<\/i><\/b> Hydrogen sulfide (H<sub>2<\/sub>S), known as a third gasotransmitter, is a signaling molecule that plays a regulatory role in physiological and pathophysiological processes. Decreased H<sub>2<\/sub>S levels were reported in inflammatory respiratory diseases such as asthma, chronic obstructive pulmonary disease, and pulmonary hypertension. H<sub>2<\/sub>S donors or drugs that increase H<sub>2<\/sub>S have emerged as novel treatments for inflammatory respiratory diseases. We previously showed that resveratrol (RVT) causes vascular relaxation and antioxidant effects by inducing H<sub>2<\/sub>S production. In the current study, we synthesized a new molecule Cpd2, as an RVT analog. We examined the effect of Cpd2 and its precursor chalcone compound (Cpd1) on H<sub>2<\/sub>S formation under both healthy and oxidative stress conditions in the lung, as well as vascular relaxation in the aorta. <b><i>Methods:<\/i><\/b> Cpd2 synthesized from Cpd1 with microwaved in basic conditions. H<sub>2<\/sub>S formation was measured by H<sub>2<\/sub>S biosensor in the mice lungs under both healthy and pyrogallol-induced oxidative stress conditions in the presence\/absence of H<sub>2<\/sub>S synthesis inhibitor aminooxyacetic acid (AOAA). The effect of compounds on vascular tonus is investigated in mice aorta by DMT myograph. <b><i>Results:<\/i><\/b> RVT and Cpd2 significantly increased <sc>l<\/sc>-cysteine (<sc>l<\/sc>-cys) induced-H<sub>2<\/sub>S formation in the lung homogenates of healthy mice, but Cpd1 did not. Superoxide anion generator pyrogallol caused a decrease in H<sub>2<\/sub>S levels in mice lungs and Cpd2 restored it. Inhibition of Cpd2-induced H<sub>2<\/sub>S formation by AOAA confirmed that Cpd2 increases endogenous H<sub>2<\/sub>S formation in both healthy and oxidative stress conditions. Furthermore, we found that both Cpd1 and Cpd2 (10<sup>\u22128<\/sup>\u201310<sup>\u22124<\/sup> M) caused vascular relaxation in mice aorta. <b><i>Discussion and Conclusion:<\/i><\/b> We found that Cpd2, a newly synthesized RVT analog, is an H<sub>2<\/sub>S-inducing molecule and vasorelaxant similar to RVT. Since H<sub>2<\/sub>S has antioxidant and anti-inflammatory effects, Cpd2 has a potential for the treatment of respiratory diseases where oxidative stress and decreased H<sub>2<\/sub>S levels are present. <\/jats:p>","DOI":"10.1159\/000533419","type":"journal-article","created":{"date-parts":[[2023,9,18]],"date-time":"2023-09-18T06:32:30Z","timestamp":1695018750000},"page":"530-539","source":"Crossref","is-referenced-by-count":2,"title":["The Effects of Novel Triazolopyrimidine Derivatives on H<sub>2<\/sub>S Production in Lung and Vascular Tonus in Aorta"],"prefix":"10.1159","volume":"108","author":[{"given":"Emine 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