{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T23:55:21Z","timestamp":1771026921210,"version":"3.50.1"},"reference-count":40,"publisher":"S. Karger AG","issue":"1","license":[{"start":{"date-parts":[[2023,8,17]],"date-time":"2023-08-17T00:00:00Z","timestamp":1692230400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"},{"start":{"date-parts":[[2023,8,17]],"date-time":"2023-08-17T00:00:00Z","timestamp":1692230400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Neuroendocrinology"],"published-print":{"date-parts":[[2024]]},"abstract":"<jats:p>&lt;b&gt;&lt;i&gt;Introduction:&lt;\/i&gt;&lt;\/b&gt; Aberrant miR-320a has been reported to be involved in the tumorigenesis of several cancers. In our previous study, we identified the low expression of circulating miR-320a in patients with somatotroph pituitary neuroendocrine tumor (PitNET); however, the role of miR-320a in somatotroph PitNET proliferation is still unclear. &lt;b&gt;&lt;i&gt;Methods:&lt;\/i&gt;&lt;\/b&gt; Cell viability and colony formation assays were used to detect the effect of miR-320a and BCAT1 on GH3 cells. TargetScan was used to identify the target genes of miR-320a. Dual-luciferase reporter gene assay was used to explore the relation between miR-320a and BCAT1. Transcriptome and proteome analyses were performed between somatotroph PitNETs and healthy controls. The expression level of miR-320a in somatotroph PitNETs were detected by RT-qPCR and Western blot. &lt;b&gt;&lt;i&gt;Results:&lt;\/i&gt;&lt;\/b&gt; miR-320a mimics inhibit cell proliferation, while miR-320a inhibitors promote cell proliferation in GH3 cells. An overlap analysis using a Venn diagram revealed that BCAT1 is the only target gene of miR-320a overexpressed in somatotroph PitNETs compared to healthy controls, as revealed by both microarray and proteomics results. A dual-luciferase reporter gene assay showed that miR-320a may bind to the BCAT1-3\u2032UTR. The transfection of miR-320a mimics downregulated the expression and miR-320a inhibitors and upregulated the expression of BCAT1 in GH3 cells. The interference of BCAT1 expression in GH3 cells downregulated cell proliferation and growth. Pan-cancer analyses demonstrated that high BCAT1 expression often indicates a poor prognosis. &lt;b&gt;&lt;i&gt;Conclusion:&lt;\/i&gt;&lt;\/b&gt; Our findings illustrate that miR-320a may function as a tumor suppressor and BCAT1 may promote tumor progression. miR-320a may inhibit the growth of somatotroph PitNETs by targeting BCAT1. <\/jats:p>","DOI":"10.1159\/000533549","type":"journal-article","created":{"date-parts":[[2023,10,11]],"date-time":"2023-10-11T21:03:05Z","timestamp":1697058185000},"page":"14-24","source":"Crossref","is-referenced-by-count":3,"title":["MiR-320a Acts as a Tumor Suppressor in Somatotroph Pituitary Neuroendocrine Tumors by Targeting BCAT1"],"prefix":"10.1159","volume":"114","author":[{"given":"Sida","family":"Zhao","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bin","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hua","family":"Gao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yazhuo","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2023,8,17]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2016.19699"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1128\/spectrum.00425-21"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1530\/EJE-08-0267"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1038\/s41572-019-0071-6"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2014-2900"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1210\/clinem\/dgac128"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1007\/s11102-016-0778-2"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1007\/s11102-016-0783-5"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1159\/000493969"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0139233"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1080\/14737159.2018.1425143"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.3390\/ijms23052867"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1007\/s10565-020-09570-0"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2021.771354"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1155\/2019\/8516858"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2017.03.004"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.3892\/mmr.2017.7819"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1177\/0300060519896144"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1007\/s40618-018-0890-4"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1039\/b504988a"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1038\/s12276-020-0475-0"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2021.645475"},{"key":"ref23","doi-asserted-by":"publisher","DOI":"10.1186\/s13008-023-00083-w"},{"key":"ref24","doi-asserted-by":"publisher","DOI":"10.1186\/s40364-021-00289-6"},{"key":"ref25","doi-asserted-by":"publisher","DOI":"10.7150\/jca.62120"},{"key":"ref26","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2021.581932"},{"key":"ref27","doi-asserted-by":"publisher","DOI":"10.18632\/aging.203258"},{"key":"ref28","doi-asserted-by":"publisher","DOI":"10.1007\/s12640-022-00530-6"},{"key":"ref29","doi-asserted-by":"publisher","DOI":"10.1111\/jgh.15657"},{"key":"ref30","doi-asserted-by":"publisher","DOI":"10.1186\/s12885-019-5815-x"},{"key":"ref31","doi-asserted-by":"publisher","DOI":"10.1016\/j.humpath.2018.02.003"},{"key":"ref32","doi-asserted-by":"publisher","DOI":"10.1126\/science.aaf5171"},{"key":"ref33","doi-asserted-by":"publisher","DOI":"10.1111\/liv.13178"},{"key":"ref34","doi-asserted-by":"publisher","DOI":"10.1038\/nature22314"},{"key":"ref35","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2021.659260"},{"key":"ref36","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2017.02.101"},{"key":"ref37","doi-asserted-by":"publisher","DOI":"10.1186\/s12891-022-05332-7"},{"key":"ref38","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-06853-3"},{"key":"ref39","doi-asserted-by":"publisher","DOI":"10.1155\/2021\/6696015"},{"key":"ref40","doi-asserted-by":"publisher","DOI":"10.1677\/ERC-08-0269"}],"container-title":["Neuroendocrinology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/karger.com\/nen\/article-pdf\/114\/1\/14\/4157150\/000533549.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/karger.com\/nen\/article-pdf\/114\/1\/14\/4157150\/000533549.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T15:37:28Z","timestamp":1745422648000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000533549"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,8,17]]},"references-count":40,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2024,1,11]]},"published-print":{"date-parts":[[2024,1,16]]}},"URL":"https:\/\/doi.org\/10.1159\/000533549","archive":["Portico"],"relation":{"is-supplemented-by":[{"id-type":"doi","id":"10.6084\/m9.figshare.23975580","asserted-by":"subject"}]},"ISSN":["0028-3835","1423-0194"],"issn-type":[{"value":"0028-3835","type":"print"},{"value":"1423-0194","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023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