{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T04:26:10Z","timestamp":1745468770589,"version":"3.40.4"},"reference-count":22,"publisher":"S. Karger AG","issue":"1","license":[{"start":{"date-parts":[[2023,12,21]],"date-time":"2023-12-21T00:00:00Z","timestamp":1703116800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2023,12,21]],"date-time":"2023-12-21T00:00:00Z","timestamp":1703116800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Ophthalmic Res"],"published-print":{"date-parts":[[2024]]},"abstract":"<b><i>Introduction:<\/i><\/b> The objective of this study was to investigate the impact of N-acetylserotonin (NAS) on the autophagy of retinal cells in rats with retinal ischemia-reperfusion injury (RIRI) and to explore the mechanisms by which NAS administration can alleviate RIRI through the tropomyosin-related kinase receptor B (TrkB)\/protein kinase B (Akt)\/nuclear factor erythroid-derived factor 2-related factor (Nrf2) signaling pathway. <b><i>Methods:<\/i><\/b> Healthy adult male rats were randomly assigned to four groups: sham, RIRI, RIRI+NAS, and RIRI+NAS+ANA-12. The RIRI group was induced by elevating intraocular pressure, and changes in retinal structure and edema were assessed using H&amp;E staining. The RIRI+NAS and RIRI+NAS+ANA-12 groups received intraperitoneal injections of NAS before and after modeling. The RIRI+NAS+ANA-12 group was also administered ANA-12, a TrkB antagonist. Immunohistochemical staining and Western blot analysis were used to evaluate phosphorylated TrkB (p-TrkB), phosphorylated Akt (p-Akt), Nrf2, sequestosome 1 (P62), and microtubule-associated protein 1 light chain 3 (LC3-II) levels in the retinas of each group. Electroretinogram was recorded to detect retinal function in each group of rats 24 h after modeling. <b><i>Results:<\/i><\/b> The RIRI+NAS group had a thinner retina and more retinal ganglion cells (RGCs) than RIRI and RIRI+NAS+ANA-12 groups (<i>p<\/i> &lt; 0.05). Immunohistochemical staining and Western blot results showed that p-TrkB, p-Akt, n-Nrf2, and P62 levels in the RIRI+NAS group were higher compared with those in RIRI and RIRI+NAS+ANA-12 groups (<i>p<\/i> &lt; 0.05). Also, lower LC3-II levels were observed in the RIRI+NAS group compared with that in RIRI and RIRI+NAS+ANA-12 groups (<i>p<\/i> &lt; 0.05). Electroretinogram recording results showed that 24 h after retinal ischemia-reperfusion, the magnitude of b-wave changes was attenuated in the RIRI+NAS group compared with the RIRI group (<i>p<\/i> &lt; 0.05). <b><i>Conclusion:<\/i><\/b> The administration of NAS activates the TrkB\/Akt\/Nrf2 signaling pathway, reduces autophagy, alleviates retinal edema, promotes the survival of retinal ganglion cells (RGCs), and provides neuroprotection against retinal injury. <\/jats:p>","DOI":"10.1159\/000535786","type":"journal-article","created":{"date-parts":[[2023,12,21]],"date-time":"2023-12-21T22:00:38Z","timestamp":1703196038000},"page":"125-136","source":"Crossref","is-referenced-by-count":0,"title":["N-Acetylserotonin Alleviates Retinal Autophagy via TrkB\/AKT\/Nrf2 Signaling Pathway in Retinal Ischemia-Reperfusion Injury Rats"],"prefix":"10.1159","volume":"67","author":[{"given":"Luming","family":"Zhang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Meng","family":"Gao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuze","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yi","family":"Yin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xuening","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuanhu","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xin","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoli","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yansong","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2023,12,21]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1080\/10520295.2017.1406616"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1080\/21655979.2021.1880060"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-018-1044-5"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1002\/tox.23389"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuint.2019.104606"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2018.10.402"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.1948-13.2014"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1177\/0963689719857649"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1039\/c9nr07171g"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.3390\/antiox9040303"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1016\/j.arr.2020.101207"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1016\/j.exer.2015.02.020"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.3390\/md18070372"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1038\/cdd.2014.143"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1016\/j.exer.2021.108595"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M110.118976"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0231212"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.14246"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.3389\/fnins.2018.00127"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2017.7342"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2016.12.034"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.14870"}],"container-title":["Ophthalmic Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/karger.com\/ore\/article-pdf\/doi\/10.1159\/000535786\/4115255\/000535786.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/karger.com\/ore\/article-pdf\/doi\/10.1159\/000535786\/4115255\/000535786.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T16:01:25Z","timestamp":1745424085000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000535786"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,12,21]]},"references-count":22,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2024,1,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000535786","archive":["Portico"],"relation":{},"ISSN":["0030-3747","1423-0259"],"issn-type":[{"type":"print","value":"0030-3747"},{"type":"electronic","value":"1423-0259"}],"subject":[],"published":{"date-parts":[[2023,12,21]]}}}