{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T20:52:39Z","timestamp":1788555159705,"version":"build-2803163510"},"reference-count":73,"publisher":"S. Karger AG","issue":"2","license":[{"start":{"date-parts":[[2024,1,1]],"date-time":"2024-01-01T00:00:00Z","timestamp":1704067200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2024,1,1]],"date-time":"2024-01-01T00:00:00Z","timestamp":1704067200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Obes Facts"],"published-print":{"date-parts":[[2024]]},"abstract":"<jats:p>&lt;b&gt;&lt;i&gt;Introduction:&lt;\/i&gt;&lt;\/b&gt; Longitudinal effect of diet-induced obesity on bone is uncertain. Prior work showed both no effect and a decrement in bone density or quality when obesity begins prior to skeletal maturity. We aimed to quantify long-term effects of obesity on bone and bone marrow adipose tissue (BMAT) in adulthood. &lt;b&gt;&lt;i&gt;Methods:&lt;\/i&gt;&lt;\/b&gt; Skeletally mature, female C57BL\/6 mice (&lt;i&gt;n&lt;\/i&gt; = 70) aged 12 weeks were randomly allocated to low-fat diet (LFD; 10% kcal fat; &lt;i&gt;n&lt;\/i&gt; = 30) or high-fat diet (HFD; 60% kcal fat; &lt;i&gt;n&lt;\/i&gt; = 30), with analyses at 12, 15, 18, and 24 weeks (&lt;i&gt;n&lt;\/i&gt; = 10\/group). Tibial microarchitecture was analyzed by \u00b5CT, and volumetric BMAT was quantified via 9.4T MRI\/advanced image analysis. Histomorphometry of adipocytes and osteoclasts, and qPCR were performed. &lt;b&gt;&lt;i&gt;Results:&lt;\/i&gt;&lt;\/b&gt; Body weight and visceral white adipose tissue accumulated in response to HFD started in adulthood. Trabecular bone parameters declined with advancing experimental age. BV\/TV declined 22% in LFD (&lt;i&gt;p&lt;\/i&gt; = 0.0001) and 17% in HFD (&lt;i&gt;p&lt;\/i&gt; = 0.0022) by 24 weeks. HFD failed to appreciably alter BV\/TV and had negligible impact on other microarchitecture parameters. Both dietary intervention and age accounted for variance in BMAT, with regional differences: distal femoral BMAT was more responsive to diet, while proximal femoral BMAT was more attenuated by age. BMAT increased 60% in the distal metaphysis in HFD at 18 and 24 weeks (&lt;i&gt;p&lt;\/i&gt; = 0.0011). BMAT in the proximal femoral diaphysis, unchanged by diet, decreased 45% due to age (&lt;i&gt;p&lt;\/i&gt; = 0.0002). Marrow adipocyte size via histomorphometry supported MRI quantification. Osteoclast number did not differ between groups. Tibial qPCR showed attenuation of some adipose, metabolism, and bone genes. A regulator of fatty acid \u03b2-oxidation, cytochrome C (CYCS), was 500% more abundant in HFD bone (&lt;i&gt;p&lt;\/i&gt; &amp;lt; 0.0001; diet effect). CYCS also increased due to age, but to a lesser extent. HFD mildly increased OCN, TRAP, and SOST. &lt;b&gt;&lt;i&gt;Conclusions:&lt;\/i&gt;&lt;\/b&gt; Long-term high fat feeding after skeletal maturity, despite upregulation of visceral adiposity, body weight, and BMAT, failed to attenuate bone microarchitecture. In adulthood, we found aging to be a more potent regulator of microarchitecture than diet-induced obesity. <\/jats:p>","DOI":"10.1159\/000536159","type":"journal-article","created":{"date-parts":[[2024,1,15]],"date-time":"2024-01-15T22:00:30Z","timestamp":1705356030000},"page":"145-157","source":"Crossref","is-referenced-by-count":7,"title":["Diet-Stimulated Marrow Adiposity Fails to Worsen Early, Age-Related Bone Loss"],"prefix":"10.1159","volume":"17","author":[{"given":"Cody","family":"McGrath","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah E.","family":"Little-Letsinger","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriel 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