{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T19:38:29Z","timestamp":1787168309307,"version":"build-2736575974"},"reference-count":54,"publisher":"S. Karger AG","license":[{"start":{"date-parts":[[2024,5,15]],"date-time":"2024-05-15T00:00:00Z","timestamp":1715731200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"},{"start":{"date-parts":[[2024,5,15]],"date-time":"2024-05-15T00:00:00Z","timestamp":1715731200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Acta Haematol"],"abstract":"<jats:p>&lt;b&gt;&lt;i&gt;Introduction:&lt;\/i&gt;&lt;\/b&gt; Major histocompatibility complex II (MHC-II)-mediated antigen presentation contributes to the pathogenesis of immune thrombocytopenia (ITP). Human leukocyte antigen (HLA)-DRB5 is an MHC-II molecule and this study aims to investigate its role and mechanisms in ITP development. &lt;b&gt;&lt;i&gt;Methods:&lt;\/i&gt;&lt;\/b&gt; Guinea pig anti-mouse platelet (PLT) serum-induced ITP mice received tail vein injection of HLA-DRB5 overexpressing adenoviral vector\/immune receptor expressed on myeloid cells-1 (IREM-1) monoclonal antibody (mAb). PLT count changes in mice blood were assessed by a hematology analyzer. MHC-II\/CD80\/CD86 expression in mice blood was measured by quantitative real-time-PCR and immunofluorescence assay. CD8&lt;sup&gt;+&lt;\/sup&gt; T-cell proportion in mice blood was detected by flow cytometry. &lt;b&gt;&lt;i&gt;Results:&lt;\/i&gt;&lt;\/b&gt; HLA-DRB5 overexpression exacerbated PLT reduction since the 5th day of the establishment of ITP mice model and enhanced MHC-II\/CD80\/CD86 expression upregulation as well as CD8&lt;sup&gt;+&lt;\/sup&gt; T-cell ratio elevation in the blood of ITP mice, while its effects were reversed by IREM-1 mAb. &lt;b&gt;&lt;i&gt;Conclusion:&lt;\/i&gt;&lt;\/b&gt; HLA-DRB5 overexpression upregulates MHC-II-mediated antigen presentation to CD8&lt;sup&gt;+&lt;\/sup&gt; T cells, thus lowering PLT count in the ITP mice model.<\/jats:p>","DOI":"10.1159\/000538749","type":"journal-article","created":{"date-parts":[[2024,5,15]],"date-time":"2024-05-15T17:00:31Z","timestamp":1715792431000},"page":"1-9","source":"Crossref","is-referenced-by-count":3,"title":["HLA-DRB5 Overexpression Promotes Platelet Reduction in Immune Thrombocytopenia Mice Model by Facilitating 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