{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T11:30:08Z","timestamp":1780054208989,"version":"3.54.0"},"reference-count":28,"publisher":"S. Karger AG","license":[{"start":{"date-parts":[[2024,7,15]],"date-time":"2024-07-15T00:00:00Z","timestamp":1721001600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2024,7,15]],"date-time":"2024-07-15T00:00:00Z","timestamp":1721001600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Transfus Med Hemother"],"abstract":"<jats:p>&lt;b&gt;&lt;i&gt;Introduction:&lt;\/i&gt;&lt;\/b&gt; Serologic characterization of antihuman platelet antigen (HPA) alloantibodies is crucial in fetal neonatal alloimmune thrombocytopenia. The gold standard MAIPA assay requires fresh platelets from HPA-genotyped donors, which is challenging for some laboratories. Megakaryocytes express HPA epitopes and offer an alternative source for detecting anti-HPA antibodies. The objective of this study was to assess the efficacy of a novel assay called monoclonal antibody immobilization of megakaryocyte antigens (MAIMA) for detecting anti-HPA antibodies. &lt;b&gt;&lt;i&gt;Methods:&lt;\/i&gt;&lt;\/b&gt; CD34+ cells from buffy coats were differentiated into megakaryocytes in vitro. The performance of the MAIMA assay was evaluated using WHO reference reagents for HPA-1a, HPA-3a, and HPA-5b, along with sera samples from patients who had well-characterized anti-HPA antibodies. &lt;b&gt;&lt;i&gt;Results:&lt;\/i&gt;&lt;\/b&gt; The WHO anti-HPA-1a reference reagent showed similar binding to megakaryocytes and platelets in MAIMA and MAIPA, respectively. On the other hand, optical density (OD) values for the WHO anti-HPA-3a reference reagent were lower in MAIMA than in MAIPA. Anti-HPA-5b antibodies were not detectable in MAIMA. Patients\u2019 sera containing anti-HPA-1a antibodies were successfully detected in MAIMA in all clinical samples. Moreover, OD values in MAIPA and MAIMA showed high correlation (&lt;i&gt;r&lt;\/i&gt; = 0.96, &lt;i&gt;p&lt;\/i&gt; &amp;lt; 0.001). MAIMA was reactive for samples with anti-HPA-3a as well as anti-HPA-3b; however, OD values were lower compared to MAIPA. Interestingly, all patient samples with anti-HPA-5b antibodies were tested negative in MAIMA. &lt;b&gt;&lt;i&gt;Conclusion:&lt;\/i&gt;&lt;\/b&gt; In vitro generated megakaryocytes can be used to detect anti-HPA-1a alloantibodies. However, despite this potential, they may be less suitable for the detection of alloantibodies against other HPAs such as HPA-5b. <\/jats:p>","DOI":"10.1159\/000539617","type":"journal-article","created":{"date-parts":[[2024,7,22]],"date-time":"2024-07-22T21:15:29Z","timestamp":1721682929000},"page":"1-10","source":"Crossref","is-referenced-by-count":2,"title":["In vitro Generated Megakaryocytes for the Detection of Human Platelet Antigen-Specific Alloantibodies"],"prefix":"10.1159","author":[{"given":"G\u00fcnalp","family":"Uzun","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Josip","family":"Lucic","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Irene","family":"Marini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Flavianna","family":"Rigoni","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Franziska","family":"Lyshy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Omid","family":"Haghighi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nina","family":"Wolska","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefanie","family":"Nowak-Harnau","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karina","family":"Althaus","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ulrich J.","family":"Sachs","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tamam","family":"Bakchoul","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"127","published-online":{"date-parts":[[2024,7,15]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2021.04.211"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1002\/pbc.26690"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1016\/S2352-3026(22)00243-5"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-0528.2010.02657.x"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1111\/vox.12085"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1016\/j.cca.2018.05.009"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1515\/labmed-2020-0012"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1016\/j.transci.2019.102705"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1182\/blood.2019002225"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1182\/blood.2022017283"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1111\/vox.12863"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.2450\/2015.0275-14"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1182\/blood.v70.6.1722.bloodjournal7061722"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2005.04218.x"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2004.04026.x"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1172\/JCI114082"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1182\/blood.v75.12.2343.bloodjournal75122343"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1067\/mpd.2001.114029"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3148.2010.01064.x"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.2003.00533.x"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2007.01550.x"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3148.2004.00537.x"},{"key":"ref23","doi-asserted-by":"publisher","DOI":"10.1172\/JCI116849"},{"key":"ref24","doi-asserted-by":"publisher","DOI":"10.1186\/s12967-018-1576-6"},{"key":"ref25","doi-asserted-by":"publisher","DOI":"10.1111\/bjh.18173"},{"key":"ref26","doi-asserted-by":"publisher","DOI":"10.2450\/2012.0126-12"},{"key":"ref27","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M009117200"},{"key":"ref28","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2141.1997.3013118.x"}],"container-title":["Transfusion Medicine and Hemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/karger.com\/tmh\/article-pdf\/doi\/10.1159\/000539617\/4252686\/000539617.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/karger.com\/tmh\/article-pdf\/doi\/10.1159\/000539617\/4252686\/000539617.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T15:24:03Z","timestamp":1745421843000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000539617"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,7,15]]},"references-count":28,"URL":"https:\/\/doi.org\/10.1159\/000539617","archive":["Portico"],"relation":{"is-supplemented-by":[{"id-type":"doi","id":"10.6084\/m9.figshare.25966024","asserted-by":"subject"}]},"ISSN":["1660-3796","1660-3818"],"issn-type":[{"value":"1660-3796","type":"print"},{"value":"1660-3818","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,7,15]]}}}