{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T03:35:28Z","timestamp":1747193728563,"version":"3.40.5"},"reference-count":22,"publisher":"S. Karger AG","issue":"5","license":[{"start":{"date-parts":[[2024,7,24]],"date-time":"2024-07-24T00:00:00Z","timestamp":1721779200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"},{"start":{"date-parts":[[2024,7,24]],"date-time":"2024-07-24T00:00:00Z","timestamp":1721779200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Acta Cytologica"],"abstract":"<jats:p>Introduction: The atypical urothelial cell (AUC) category in The Paris System (TPS) in urine cytology (UrCy) is a challenging area. This study aimed to evaluate the UroVysion fluorescence in situ hybridization (U-FISH) assay in predicting the outcome of AUC. Additionally, we explored the association of abnormal U-FISH results in high-grade urothelial carcinoma (HGUC) concerning muscularis propria invasion (MPI). Methods: This is a retrospective study, and U-FISH was done on archived Papanicolaou-stained smears. Four cohorts were included: non-neoplastic AUC (AUC-NN), neoplastic AUC (AUC-N), muscle-invasive HGUC (HGUC-MI), and muscle-free HGUC (HGUC-MF) outcome on histopathology (HPE) and with clinical follow-up of 12\u201329 months. U-FISH was evaluated for diagnostic purposes, and MPI and tumor stage prediction by urine FISH score (UFS; high vs. low) based on copy number gain of chromosomes (Chr). Results: U-FISH was performed on 70 cases (20 AUC-NN, 20 AUC-N, 15 HGUC-MI, and 15 HGUC-MF) and was successful in 58\/70 (82.85%) cases. All UC cases showed polysomy of \u22652Chr, and all the AUC-NN cases reported non-neoplastic on HPE were negative for U-FISH. U-FISH picked up all carcinoma cases in the AUC-N cohort. Chr 3 polysomy was statistically significant in differentiating HGUC-MI from HGUC-MF and low-grade urothelial carcinoma cases. Chr 3 signals with a cut-off of 6 signals could identify MPI with a sensitivity of 80.95% and specificity of 41.94%. The UFS of the HGUC-MI group was significantly higher than HGUC-MF. Conclusions: U-FISH successfully identified all cases of AUC with neoplastic outcomes. In the HGUC group, there was a difference in cases with and without MPI, which requires further confirmation in a larger prospective cohort. <\/jats:p>","DOI":"10.1159\/000540070","type":"journal-article","created":{"date-parts":[[2024,7,24]],"date-time":"2024-07-24T21:02:59Z","timestamp":1721854979000},"page":"423-435","source":"Crossref","is-referenced-by-count":0,"title":["Utility of UroVysion Fluorescence in situ Hybridization in Improving the Diagnostic Performance of Urine Cytology"],"prefix":"10.1159","volume":"68","author":[{"given":"K.C.","family":"Sharan","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Radhika","family":"Srinivasan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Radha","family":"Uppal","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manish","family":"Rohilla","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9297-5400","authenticated-orcid":false,"given":"Pranab","family":"Dey","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nandita","family":"Kakkar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ravimohan S.","family":"Mavuduru","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2024,7,24]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.20006"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1016\/j.urology.2006.03.062"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1309\/K5P2-4Y8B-7L5A-FAA9"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.21704"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1097\/01.ju.0000066842.45464.cc"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1136\/jclinpath-2019-206109"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1245\/s10434-016-5763-5"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1002\/cncy.20171"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1093\/ajcp\/aqz165"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.2353\/jmoldx.2009.080096"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1309\/ADJL7E810U1H42BJ"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1111\/cyt.12604"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1159\/000499027"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1016\/S1525-1578(10)60625-3"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1016\/j.cccn.2003.11.008"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1097\/PAP.0b013e3181832320"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1159\/000323652"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1016\/j.juro.2008.01.105"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1002\/cncy.20016"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1111\/j.1464-410X.2005.05509.x"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1002\/dc.10291"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5347(05)64270-X"}],"container-title":["Acta Cytologica"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/karger.com\/acy\/article-pdf\/68\/5\/423\/4297169\/000540070.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/karger.com\/acy\/article-pdf\/68\/5\/423\/4297169\/000540070.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T15:28:26Z","timestamp":1745422106000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000540070"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,7,24]]},"references-count":22,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2024,11,5]]},"published-print":{"date-parts":[[2024,11,6]]}},"URL":"https:\/\/doi.org\/10.1159\/000540070","archive":["Portico"],"relation":{},"ISSN":["0001-5547","1938-2650"],"issn-type":[{"type":"print","value":"0001-5547"},{"type":"electronic","value":"1938-2650"}],"subject":[],"published":{"date-parts":[[2024,7,24]]}}}