{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,8]],"date-time":"2025-07-08T05:47:40Z","timestamp":1751953660086,"version":"3.40.5"},"reference-count":10,"publisher":"S. Karger AG","license":[{"start":{"date-parts":[[2024,8,13]],"date-time":"2024-08-13T00:00:00Z","timestamp":1723507200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"},{"start":{"date-parts":[[2024,8,13]],"date-time":"2024-08-13T00:00:00Z","timestamp":1723507200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Neonatology"],"abstract":"<jats:p>&lt;b&gt;&lt;i&gt;Introduction:&lt;\/i&gt;&lt;\/b&gt; Intrauterine herpes simplex virus (HSV) infection is uncommon and challenging to diagnose, requiring detection of HSV in skin lesions within 48 h post-birth. &lt;b&gt;&lt;i&gt;Case Presentation:&lt;\/i&gt;&lt;\/b&gt; A preterm female infant presented with the typical triad of blisters, microcephaly, and chorioretinitis, but the initial diagnostic approach was elusive due to negative results for TORCH pathogens from vesicles\/serum. Referred at 7 months for developmental delay and epilepsy, her brain imaging showed calcification and cortical dysplasia. Polymerase chain reaction (PCR) of her preserved dried umbilical cord detected HSV-2 DNA, diagnosing intrauterine HSV infection. HSV-2 was later found in relapsed blisters at 8 months but not in cerebrospinal fluid or brain tissue. A literature review identified 104 congenital\/intrauterine HSV cases; 28.8% presented the typical triad, and 50% were diagnosed using specimens collected 48 h post-birth. &lt;b&gt;&lt;i&gt;Conclusion:&lt;\/i&gt;&lt;\/b&gt; This case marks the first retrospective diagnosis of intrauterine HSV infection via PCR on preserved umbilical cord, underscoring its diagnostic value. <\/jats:p>","DOI":"10.1159\/000540506","type":"journal-article","created":{"date-parts":[[2024,8,13]],"date-time":"2024-08-13T21:01:15Z","timestamp":1723582875000},"page":"1-5","source":"Crossref","is-referenced-by-count":1,"title":["Diagnostic Utility of Preserved Dried Umbilical Cord Polymerase Chain Reaction in Intrauterine Herpes Simplex Virus Infection: A Case Report and Literature Review"],"prefix":"10.1159","author":[{"given":"Yasumasa","family":"Tsuda","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takeshi","family":"Matsushige","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hirofumi","family":"Inoue","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Madoka","family":"Hoshide","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1367-2554","authenticated-orcid":false,"given":"Hiroki","family":"Hamano","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Keiko","family":"Hasegawa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masako","family":"Moriuchi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroyuki","family":"Moriuchi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shunji","family":"Hasegawa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2024,8,13]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1053\/j.semperi.2018.02.004"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.17.1.1-13.2004"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1093\/jpids\/piw013"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1097\/INF.0b013e3181f55a5c"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpeds.2009.04.033"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1016\/j.braindev.2020.01.003"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1097\/00006454-200405000-00028"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1097\/01.inf.0000168842.56185.cd"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1016\/j.pediatrneurol.2005.10.006"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1159\/000442972"}],"container-title":["Neonatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/karger.com\/neo\/article-pdf\/doi\/10.1159\/000540506\/4266213\/000540506.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/karger.com\/neo\/article-pdf\/doi\/10.1159\/000540506\/4266213\/000540506.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T15:32:30Z","timestamp":1745422350000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000540506"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,8,13]]},"references-count":10,"URL":"https:\/\/doi.org\/10.1159\/000540506","archive":["Portico"],"relation":{"is-supplemented-by":[{"id-type":"doi","id":"10.6084\/m9.figshare.26362588","asserted-by":"subject"}]},"ISSN":["1661-7800","1661-7819"],"issn-type":[{"type":"print","value":"1661-7800"},{"type":"electronic","value":"1661-7819"}],"subject":[],"published":{"date-parts":[[2024,8,13]]}}}