{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,20]],"date-time":"2026-05-20T14:19:03Z","timestamp":1779286743629,"version":"3.51.4"},"reference-count":34,"publisher":"S. Karger AG","issue":"3","license":[{"start":{"date-parts":[[2024,10,16]],"date-time":"2024-10-16T00:00:00Z","timestamp":1729036800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"},{"start":{"date-parts":[[2024,10,16]],"date-time":"2024-10-16T00:00:00Z","timestamp":1729036800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pharmacology"],"abstract":"<jats:p>Introduction: Melanocyte ferroptosis has been proven to contribute to the development of vitiligo. Tanshinone IIA (TSA), a Chinese herbal extract, has been shown to inhibit vitiligo progression. Whether TSA regulates ferroptosis in melanocytes remains unclear. Methods: Hydrogen peroxide (H&lt;sub&gt;2&lt;\/sub&gt;O&lt;sub&gt;2&lt;\/sub&gt;) was used to induce melanocytes to stimulate vitiligo cell model in vitro. Cell proliferation was examined by 5-ethynyl-2\u2032-deoxyuridine assay. The levels of malondialdehyde, reactive oxygen species, glutathione peroxidase, and iron were detected by corresponding commercial kit. The protein levels of ferroptosis-related markers and Nrf2 pathway-related markers were examined using western blot and immunofluorescence staining. Cell viability and cytotoxicity were analyzed using Cell Counting Kit-8 assay and lactate dehydrogenase detection. Mitochondrial morphology was examined using a transmission electron microscope. Results: After H&lt;sub&gt;2&lt;\/sub&gt;O&lt;sub&gt;2&lt;\/sub&gt; treatment, melanocyte proliferation was reduced, while oxidative stress and ferroptosis were enhanced. TSA treatment could inhibit ferroptosis in H&lt;sub&gt;2&lt;\/sub&gt;O&lt;sub&gt;2&lt;\/sub&gt;-induced melanocytes. Besides, TSA could activate Nrf2 pathway and promote Nrf2 nuclear translocation, and Nrf2-specific inhibitor (ML385) also reversed the inhibitory effect of TSA on H&lt;sub&gt;2&lt;\/sub&gt;O&lt;sub&gt;2&lt;\/sub&gt;-induced melanocyte ferroptosis. Conclusion: Our data showed that TSA alleviated H&lt;sub&gt;2&lt;\/sub&gt;O&lt;sub&gt;2&lt;\/sub&gt;-induced melanocyte ferroptosis via activating Nrf2 pathway. <\/jats:p>","DOI":"10.1159\/000541177","type":"journal-article","created":{"date-parts":[[2024,10,16]],"date-time":"2024-10-16T12:02:55Z","timestamp":1729080175000},"page":"141-150","source":"Crossref","is-referenced-by-count":7,"title":["Tanshinone IIA Inhibits Hydrogen Peroxide-Induced Ferroptosis in Melanocytes through Activating Nrf2 Signaling Pathway"],"prefix":"10.1159","volume":"110","author":[{"given":"Xiaosha","family":"Li","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shiyang","family":"Tang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haizhen","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xin","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2024,10,16]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1002\/med.21754"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1111\/pcmr.12955"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.12659\/MSM.914898"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.7150\/thno.30398"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1111\/pcmr.13032"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1155\/2022\/8498472"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2021.05.010"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.2147\/DDDT.S266911"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1016\/j.phrs.2020.105364"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1111\/dth.15086"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1039\/c8ra09786k"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2021.08.067"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.26508\/lsa.202201667"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2017.7342"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.3390\/molecules25225474"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1016\/j.abb.2020.108670"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1002\/hep.28251"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1007\/s10735-021-09996-8"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1016\/j.jdermsci.2019.03.007"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1016\/j.det.2016.11.014"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1186\/s13045-019-0720-y"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.7150\/thno.54113"},{"key":"ref23","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2018.09.014"},{"key":"ref24","doi-asserted-by":"publisher","DOI":"10.1002\/iid3.492"},{"key":"ref25","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2019.108599"},{"key":"ref26","doi-asserted-by":"publisher","DOI":"10.1016\/j.phrs.2021.105661"},{"key":"ref27","doi-asserted-by":"publisher","DOI":"10.1042\/BSR20201807"},{"key":"ref28","doi-asserted-by":"publisher","DOI":"10.1002\/tox.23388"},{"key":"ref29","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2019.101107"},{"key":"ref30","doi-asserted-by":"publisher","DOI":"10.18632\/aging.103378"},{"key":"ref31","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2020.10.323"},{"key":"ref32","doi-asserted-by":"publisher","DOI":"10.1159\/000489688"},{"key":"ref33","doi-asserted-by":"publisher","DOI":"10.1016\/j.rvsc.2021.03.004"},{"key":"ref34","doi-asserted-by":"publisher","DOI":"10.2174\/0115665240263196230920161019"}],"container-title":["Pharmacology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/karger.com\/pha\/article-pdf\/doi\/10.1159\/000541177\/4301529\/000541177.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/karger.com\/article-pdf\/doi\/10.1159\/000541177","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,9,19]],"date-time":"2025-09-19T16:04:44Z","timestamp":1758297884000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000541177"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,10,16]]},"references-count":34,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2025,7,1]]},"published-print":{"date-parts":[[2025,7,2]]}},"URL":"https:\/\/doi.org\/10.1159\/000541177","archive":["Portico"],"relation":{},"ISSN":["0031-7012","1423-0313"],"issn-type":[{"value":"0031-7012","type":"print"},{"value":"1423-0313","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,10,16]]}}}