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Human papillomavirus (HPV) infection is known to be the primary etiological factor in the development of cervical cancer. The identification of high-risk HPV (HR-HPV) infections in cervical cancer screening is becoming crucial for early detection and management of cervical pre-neoplasia. However, HPV testing requires triage markers since HR-HPV infections are widespread in the target population, of which only a small fraction requires detailed clinical workup by colposcopy. Summary: p16INK4a is a tumor suppressor protein that has emerged as a promising surrogate marker for HPV infection. In combination with a proliferation marker Ki67 in one diagnostic test kit referred to as CINtecPlus\u00ae or dual stain (DS), it revealed excellent sensitivity and specificity profiles to detect clinically relevant CIN2+ lesions among HPV-infected women in large screening cohorts. Key Message: This review examines the role of the cytology CINtecPlus\u00ae test (DS test) as a triage marker to identify patients with CIN2+ lesions among HPV+ tested women and summarizes the most recent recommendations for its clinical use. <\/jats:p>","DOI":"10.1159\/000542504","type":"journal-article","created":{"date-parts":[[2025,1,17]],"date-time":"2025-01-17T17:00:46Z","timestamp":1737133246000},"page":"16-25","source":"Crossref","is-referenced-by-count":9,"title":["p16<sup>INK4a<\/sup>\/Ki67 Dual-Staining Immunocytochemistry to Refer Women Infected by High-Risk Human Papillomavirus for Colposcopy"],"prefix":"10.1159","volume":"69","author":[{"given":"Magnus","family":"von Knebel 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