{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,6]],"date-time":"2025-05-06T04:05:15Z","timestamp":1746504315692,"version":"3.40.4"},"reference-count":27,"publisher":"S. Karger AG","issue":"2","license":[{"start":{"date-parts":[[2024,12,30]],"date-time":"2024-12-30T00:00:00Z","timestamp":1735516800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"},{"start":{"date-parts":[[2024,12,30]],"date-time":"2024-12-30T00:00:00Z","timestamp":1735516800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Acta Cytologica"],"abstract":"<jats:p>Introduction: Endoscopic ultrasound-guided fine-needle aspiration (EUS-FNA) is a highly sensitive and accurate method for identifying pancreatic neuroendocrine tumors (PanNETs). However, research on grading and assessing the Ki67 proliferation index in FNA samples is limited. Methods: This study analyzed 335 EUS-FNA cases performed between 2016 and 2022, of which 12 cases of PanNET were further evaluated. The morphology, Ki-67 index, and grading (G) of cell blocks (CBs) obtained from the PanNET aspirations were compared to those of the resected material. Results: Out of 12 PanNET\u2019s with rapid on-site evaluation (ROSE), in FNA samples and CB, 7 (58.3%) cases were G1, while 5 (41.7%) cases were G2. On the other hand, on resection of these cases, 6 (50%) were diagnosed as G1, 5 (41.7%) cases were G2, and 1 case (8.3%) was G3. The average Ki-67 index in CB was 2.92 (min: 1 \u2013 max: 10), while in resections it was 4.67 (min: 1 \u2013 max: 22). Only 2 (16.6%) showed a discordance between grade and Ki-67, resulting in an overall concordance of over 80%. On average, 1.83 needle passes were made (range: 1\u20133), while the average number of slides and CBs were 9.33 (range: 1\u201324) and 2.17 (range: 1\u20136), respectively. There were no significant differences in the number of passes, slides, or CBs between the consistent and discordant groups. Conclusion: Optimal counting techniques and sensitivity for Ki67 are crucial in grading PanNETs in both aspiration and resection materials. The grade and Ki-67 index demonstrated high concordance when comparing CB and resection. However, in low-volume centers, ROSE support can be beneficial in obtaining sufficient slides and CB for an adequate diagnosis. <\/jats:p>","DOI":"10.1159\/000543364","type":"journal-article","created":{"date-parts":[[2024,12,30]],"date-time":"2024-12-30T17:00:24Z","timestamp":1735578024000},"page":"122-130","source":"Crossref","is-referenced-by-count":0,"title":["Endoscopic Ultrasound-Guided Fine-Needle Aspiration of Pancreatic Neuroendocrine Tumors with Rapid On-Site Evaluation: Single-Center Experience"],"prefix":"10.1159","volume":"69","author":[{"given":"Irem Guvendir","family":"Bakkaloglu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Begum Calim","family":"Gurbuz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Onur","family":"Sahin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Itir Ebru","family":"Zemheri","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2024,12,30]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.3978\/j.issn.2227-684X.2014.08.02"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1007\/s10620-011-1912-7"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.3390\/jcm10122638"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1038\/modpathol.2014.156"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1097\/PAS.0000000000000089"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1097\/MPA.0000000000001157"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1111\/his.13975"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1007\/s12022-022-09708-2"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1055\/a-0990-9611"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.4103\/EUS-D-20-00261"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1530\/ERC-16-0464"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-022-09923-1"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2303.2006.00311.x"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1007\/s12022-018-9554-3"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1016\/j.gie.2022.07.014"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1001\/jamaoncol.2017.0589"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2002.10.030"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1111\/apm.13190"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.3390\/cancers14020433"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1097\/PAS.0b013e31821a0696"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1016\/j.critrevonc.2016.03.013"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1007\/s12020-023-03336-4"},{"key":"ref23","doi-asserted-by":"publisher","DOI":"10.1093\/ajcp\/aqz110"},{"key":"ref24","doi-asserted-by":"publisher","DOI":"10.1111\/1751-2980.12976"},{"key":"ref25","doi-asserted-by":"publisher","DOI":"10.1097\/MPA.0000000000000735"},{"key":"ref26","doi-asserted-by":"publisher","DOI":"10.1002\/dc.23662"},{"key":"ref27","doi-asserted-by":"publisher","DOI":"10.1038\/s41379-022-01055-1"}],"container-title":["Acta Cytologica"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/karger.com\/article-pdf\/doi\/10.1159\/000543364","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/karger.com\/article-pdf\/doi\/10.1159\/000543364","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,5,5]],"date-time":"2025-05-05T16:01:50Z","timestamp":1746460910000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000543364"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,12,30]]},"references-count":27,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2025,5,5]]}},"URL":"https:\/\/doi.org\/10.1159\/000543364","archive":["Portico"],"relation":{},"ISSN":["0001-5547","1938-2650"],"issn-type":[{"type":"print","value":"0001-5547"},{"type":"electronic","value":"1938-2650"}],"subject":[],"published":{"date-parts":[[2024,12,30]]}}}