{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,11]],"date-time":"2025-12-11T21:06:51Z","timestamp":1765487211512,"version":"3.46.0"},"reference-count":24,"publisher":"S. Karger AG","issue":"12","license":[{"start":{"date-parts":[[2025,3,12]],"date-time":"2025-03-12T00:00:00Z","timestamp":1741737600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2025,3,12]],"date-time":"2025-03-12T00:00:00Z","timestamp":1741737600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int Arch Allergy Immunol"],"abstract":"<jats:p>&lt;p&gt;Introduction: This study aimed to investigate the role of Raf kinase inhibitor protein (RKIP) in degranulation induced by echinococcal cyst fluid (EgCF) in bone marrow-derived mast cells (BMMCs). Methods: Primary BMMCs were isolated and cultured from the femurs and tibias of RKIP gene knockout (KO) and wild-type (WT) C57BL\/6 mice. EgCF-induced degranulation models were established for both groups. Samples of cells and supernatant were collected for analysis. Surface expression levels of CD117 and Fc-epsilon Receptor \u2160 \u03b1 (Fc\u03b5RI\u03b1) were assessed. Supernatant concentrations of \u03b2-hexosaminidase, IL-4, IL-6, and tumour necrosis factor (TNF-\u03b1) were measured. Cellular mRNA levels of IL-4, IL-6, and TNF-\u03b1 were quantified, and changes in RKIP protein expression during degranulation in WT BMMCs were monitored. Results: After 4 weeks of induction culture, the double-positive rates for CD117 and Fc\u03b5RI\u03b1 exceeded 98% in both KO and WT BMMCs. Following sensitization, BMMCs from the KO group demonstrated significantly higher degranulation rates compared to the WT group (p &lt; 0.05). In WT BMMCs, surface RKIP protein expression progressively decreased at 1 h, 2 h, and 3 h post-sensitization, corresponding with degranulation progression (p &lt; 0.05). The KO group exhibited elevated release of BMMC-related cytokines, including IL-4, IL-6, and TNF-\u03b1, compared to the WT group after sensitization (p &lt; 0.05). Similarly, transcription levels of cytokines IL-4, IL-6, and TNF-\u03b1 were higher in the KO group than in the WT group following sensitization (p &lt; 0.05). Conclusion: RKIP gene KO resulted in an increased EgCF-induced release of cytokines and bioactive substances by BMMCs, indicating that RKIP may suppress EgCF-induced BMMC degranulation. These findings suggest that RKIP could serve as a potential therapeutic target for managing allergic reactions associated with cystic echinococcosis. &lt;\/p&gt;<\/jats:p>","DOI":"10.1159\/000545176","type":"journal-article","created":{"date-parts":[[2025,3,12]],"date-time":"2025-03-12T13:02:02Z","timestamp":1741784522000},"page":"1111-1118","source":"Crossref","is-referenced-by-count":1,"title":["Role of Raf Kinase Inhibitor Protein in Modulating Echinococcal Cyst Fluid-Induced Degranulation in Bone Marrow-Derived Mast Cells"],"prefix":"10.1159","volume":"186","author":[{"given":"Shan-Shan","family":"Li","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xue-Li","family":"Pu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jing-Ru","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chun-Sheng","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jia-Ling","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xilizati","family":"Kulaixi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jian-Rong","family":"Ye","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2025,3,12]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02420-15"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1097\/MD.0000000000027552"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1007\/s12016-004-0004-2"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2020.603050"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.3390\/cancers13246247"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1615\/critrevoncog.2014011950"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1805474115"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1016\/j.molimm.2014.01.018"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2024.1418897"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1111\/his.14897"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2006.10.001"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.3390\/biom9120769"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2024.07.002"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1080\/15321819.2020.1769129"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.2337\/db23-0862"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1016\/j.pharmthera.2019.03.008"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2022.924244"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2018.01067"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-0716-0696-4_24"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.2332\/allergolint.R-07-145"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1007\/s12026-008-8042-0"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2222.2011.03953.x"},{"key":"ref23","doi-asserted-by":"publisher","DOI":"10.3390\/ijms23020706"},{"key":"ref24","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.21.21.7207-7217.2001"}],"container-title":["International Archives of Allergy and Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/karger.com\/iaa\/article-pdf\/doi\/10.1159\/000545176\/4350947\/000545176.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/karger.com\/article-pdf\/doi\/10.1159\/000545176","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T17:11:11Z","timestamp":1764609071000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000545176"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,3,12]]},"references-count":24,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2025,12,1]]},"published-print":{"date-parts":[[2025,12,3]]}},"URL":"https:\/\/doi.org\/10.1159\/000545176","archive":["Portico"],"relation":{"is-supplemented-by":[{"id-type":"doi","id":"10.6084\/m9.figshare.28581434","asserted-by":"subject"}]},"ISSN":["1018-2438","1423-0097"],"issn-type":[{"type":"print","value":"1018-2438"},{"type":"electronic","value":"1423-0097"}],"subject":[],"published":{"date-parts":[[2025,3,12]]}}}