{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T02:25:47Z","timestamp":1792808747127,"version":"4.5.0"},"reference-count":26,"publisher":"S. Karger AG","issue":"2","license":[{"start":{"date-parts":[[2025,6,18]],"date-time":"2025-06-18T00:00:00Z","timestamp":1750204800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"},{"start":{"date-parts":[[2025,6,18]],"date-time":"2025-06-18T00:00:00Z","timestamp":1750204800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Gynecol Obstet Invest"],"abstract":"<jats:p>Objectives: Endometriotic stromal cells (ESCs) are extensively found in endometriosis (EM). This study aims to investigate the effects and regulatory mechanisms of KLF10 on the proliferation of ESCs in EM. Methods: Human ESCs from eutopic and ectopic endometrium were isolated and identified. Levels of KLF10, miR-200c-3p, and lncRNA NEAT1 in cells were detected by reverse transcription-quantitative polymerase chain reaction and Western blot analysis. Expression of KLF10, miR-200c-3p, and NEAT1 were silenced in ectopic ESCs, followed by an assessment of cell proliferation. Chromatin immunoprecipitation and dual-luciferase reporter assays were conducted to analyze the binding of KLF10 to the miR-200c-3p promoter. RNA immunoprecipitation and dual-luciferase reporter assays were performed to analyze the interaction between miR-200c-3p and NEAT1. NEAT1 RNA stability was measured. Results: Compared to Eut-ESCs, Ect-ESCs exhibited decreased KLF10 and miR-200c-3p expression and increased NEAT1 expression. Overexpression of KLF10 inhibited the proliferation of Ect-ESCs. Mechanistically, KLF10 transcriptionally promoted miR-200c-3p expression, reducing the binding of miR-200c-3p to NEAT1 and downregulating NEAT1 expression. Combined experimental results showed that miR-200c-3p downregulation or NEAT1 overexpression could alleviate the inhibitory effect of KLF10 overexpression on the proliferation of Ect-ESCs. Limitations: We only investigated the function of KLF10 in Ect-ESC proliferation of EM on the cellular level, but the effect of KLF10 on abnormal Ect-ESC migration and invasion remains to be explored. Besides, there is no interference experiments performed on Eut-ESCs, and no animal experiment was included. Conclusions: KLF10 transcriptionally promoted miR-200c-3p expression reduced the binding of miR-200c-3p to NEAT1, thus downregulating NEAT1 expression and inhibiting the proliferation of Ect-ESCs.<\/jats:p>","DOI":"10.1159\/000546836","type":"journal-article","created":{"date-parts":[[2025,6,19]],"date-time":"2025-06-19T12:00:51Z","timestamp":1750334451000},"page":"210-219","source":"Crossref","is-referenced-by-count":3,"title":["Mechanisms of KLF10 in Regulating Proliferation of Endometriotic Stromal Cells in Endometriosis"],"prefix":"10.1159","volume":"91","author":[{"given":"Boyao","family":"Xia","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shan","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"127","published-online":{"date-parts":[[2025,6,18]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1097\/AOG.0000000000002469"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1093\/biolre\/ioy128"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1210\/er.2018-00242"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2022.1037504"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.116.140764"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.7150\/ijbs.45999"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.3390\/biomedicines11113087"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.18632\/aging.204396"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.15833"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.5635"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2017.01.009"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1007\/s13258-021-01184-y"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1007\/s11626-023-00760-8"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(21)00389-5"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.21873\/invivo.13356"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbadis.2023.166784"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1016\/j.rbmo.2022.02.008"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1016\/j.gene.2023.148027"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.23937\/2378-3648\/1410017"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1177\/0960327121991899"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1016\/j.jogoh.2021.102157"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrep.2022.101316"},{"key":"ref23","doi-asserted-by":"publisher","DOI":"10.1186\/s13287-017-0706-z"},{"key":"ref24","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajpath.2017.01.017"},{"key":"ref25","doi-asserted-by":"publisher","DOI":"10.2147\/OTT.S220306"},{"key":"ref26","doi-asserted-by":"publisher","DOI":"10.1155\/2020\/3962953"}],"container-title":["Gynecologic and Obstetric Investigation"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/karger.com\/article-pdf\/doi\/10.1159\/000546836","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/karger.com\/article-pdf\/doi\/10.1159\/000546836","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,9]],"date-time":"2026-04-09T16:01:05Z","timestamp":1775750465000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000546836"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,6,18]]},"references-count":26,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2026,4,9]]},"published-print":{"date-parts":[[2026,4,8]]}},"URL":"https:\/\/doi.org\/10.1159\/000546836","archive":["Portico"],"relation":{"is-supplemented-by":[{"id-type":"doi","id":"10.6084\/m9.figshare.29314448","asserted-by":"subject"}]},"ISSN":["0378-7346","1423-002X"],"issn-type":[{"value":"0378-7346","type":"print"},{"value":"1423-002X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,6,18]]}}}