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Karger AG","issue":"4","license":[{"start":{"date-parts":[[2026,1,20]],"date-time":"2026-01-20T00:00:00Z","timestamp":1768867200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"},{"start":{"date-parts":[[2026,1,20]],"date-time":"2026-01-20T00:00:00Z","timestamp":1768867200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathobiology"],"abstract":"<jats:p>Introduction: High inter- and intraobserver variability is acknowledged in negative for intraepithelial lesion or malignancy (NILM), atypical squamous cells of undetermined significance (ASC-US), and low-grade squamous intraepithelial lesion (LSIL) diagnostic categories in cervical cytology. As digital cytology has emerged, whole-slide images (WSIs) have been compared to conventional light microscopy (LM) to evaluate its feasibility in routine practice. Methods: Six cytopathologists evaluated 20 liquid-based cervical samples diagnosed as ASC-US, LSIL, or NILM with known hrHPV status, both by conventional LM and WSI, equalling 240 evaluations. Interobserver agreement was measured using Fleiss\u2019s kappa, Kendall\u00b4s coefficient and interclass correlation (ICC). Results: Most of the samples (93.75%) were assigned to the same original range of categories: NILM 5 (25%), ASC-US 10 (50%), and LSIL 5 (25%). WSI versus LM interobserver agreement varied with Fleiss\u2019s kappa (0.324 versus 0.319), Kendall\u2019s W (0.670 versus 0.572), and ICC (0.585 versus 0.414). Interobserver agreement for the ASC-US category was higher in Kendall\u2019s W (0.435 for WSI versus 0.329 for LM) and in ICC (0.394 versus 0.118). Fleiss\u2019s kappa showed lower interobserver agreement for ASC-US in WSI than LM (0.000 versus 0.039). Conclusion: This study illustrated that despite overall better agreement in WSI, ASC-US results were less favourable in WSI than LM, according to Fleiss\u2019s kappa test, but not other tests.<\/jats:p>","DOI":"10.1159\/000550455","type":"journal-article","created":{"date-parts":[[2026,1,20]],"date-time":"2026-01-20T17:00:58Z","timestamp":1768928458000},"page":"221-231","source":"Crossref","is-referenced-by-count":1,"title":["ThinPrep\u00ae Whole-Slide Digital Images versus Conventional Microscopy in Negative for Intraepithelial Lesion or Malignancy, Atypical Squamous Cells of Undetermined Significance, and Low-Grade Squamous Intraepithelial Lesion Cervical Lesions: European Federation of Cytological Societies (EFCS) 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