{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,20]],"date-time":"2026-03-20T18:02:38Z","timestamp":1774029758676,"version":"3.50.1"},"reference-count":36,"publisher":"S. Karger AG","license":[{"start":{"date-parts":[[2026,1,27]],"date-time":"2026-01-27T00:00:00Z","timestamp":1769472000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,1,27]],"date-time":"2026-01-27T00:00:00Z","timestamp":1769472000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int Arch Allergy Immunol"],"abstract":"<jats:p>Introduction: Venom immunotherapy (VIT) is the most effective treatment approach for preventing severe systemic reactions caused by hymenoptera stings. In some cases, the detection of specific immunoglobulin E (IgE) positivity for both honeybee and wasp venoms can create diagnostic and therapeutic challenges. In this study, we aimed to evaluate the effectiveness of the Immulite 2000 3g Allergy component-based diagnostic test (CBDT) in determining true sensitization in patients with dual venom positivity who were scheduled for VIT. Methods: In this prospective study, 66 patients who presented to the Immunology and Allergy Diseases outpatient clinic between January 2019 and December 2024 with a history of systemic reaction following a bee sting and who were indicated for VIT were evaluated. Results: The mean age of the patients was 46.3 \u00b1 12.9 years, with a male predominance. Honeybee stings were the most common (50%). Forty-two patients had single-venom sensitization, while 22 had specific IgE positivity for both Apis mellifera and Vespula vulgaris. According to the CBDT results, 6 of the 22 patients had dual sensitization, while 7 patients had sensitization only to Vespula. Patients with indeterminate venom sensitization had higher Api m 1\/Ves v 5 specific IgE ratios. Conclusion: In our study, using the Immulite 2000 3g Allergy CBDT results, venom sensitization could not be clearly determined in 41% of patients with dual sensitization to venom allergens, and true double sensitization was identified in only 27% of these patients. Therefore, we suggest that including additional species-specific antigens (especially Ves v 1) alongside in the Immulite 2000 3g Allergy CBDT panel could improve the detection of true sensitization. Furthermore, when supported by accurate and reliable clinical history, a specific IgE Api m 1\/Ves v 5 ratio greater than 5 can represent a significant and useful hypothesis. However, further studies are needed to confirm this hypothesis.<\/jats:p>","DOI":"10.1159\/000550719","type":"journal-article","created":{"date-parts":[[2026,1,27]],"date-time":"2026-01-27T17:02:23Z","timestamp":1769533343000},"page":"1-10","source":"Crossref","is-referenced-by-count":0,"title":["The Clinical Importance of Component-Based Diagnostic Test in Patients Planned for Venom Immunotherapy"],"prefix":"10.1159","author":[{"given":"Iclal","family":"Hocanli","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fikriye","family":"Kalkan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ozge","family":"Ozturk Aktas","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Filiz","family":"Akbiyik","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sadan","family":"Soyyigit","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2026,1,27]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.5414\/ALX02430E"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1097\/ACI.0b013e32830638c5"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1111\/all.13262"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1186\/1710-1492-9-33"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2222.2004.01897.x"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.2006.01232.x"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1186\/1710-1492-5-11"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1159\/000024453"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1111\/pai.13854"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1186\/s12865-024-00616-0"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1186\/s13052-024-01731-9"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2010.06.042"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2011.12.975"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2005.12.1331"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.0803709"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2222.2012.03966.x"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.2011.02667.x"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0062009"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.2005.00963.x"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.2008.01794.x"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1067\/mai.2001.120013"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1007\/s40629-020-00151-5"},{"key":"ref23","doi-asserted-by":"publisher","DOI":"10.1159\/000236534"},{"key":"ref24","doi-asserted-by":"publisher","DOI":"10.1067\/mai.2003.173"},{"key":"ref25","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2013.10.060"},{"key":"ref26","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaip.2018.04.045"},{"key":"ref27","doi-asserted-by":"publisher","DOI":"10.3109\/15569527.2011.621080"},{"key":"ref28","doi-asserted-by":"publisher","DOI":"10.1111\/ddg.13240"},{"key":"ref29","doi-asserted-by":"publisher","DOI":"10.1111\/all.13693"},{"key":"ref30","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaip.2018.08.012"},{"key":"ref31","doi-asserted-by":"publisher","DOI":"10.1007\/s00105-014-2776-5"},{"key":"ref32","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2009.08.037"},{"key":"ref33","doi-asserted-by":"publisher","DOI":"10.1016\/j.molimm.2009.10.005"},{"key":"ref34","doi-asserted-by":"publisher","DOI":"10.1097\/ACI.0000000000000574"},{"key":"ref35","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaip.2024.10.029"},{"key":"ref36","doi-asserted-by":"publisher","DOI":"10.1016\/j.cca.2015.12.026"}],"container-title":["International Archives of Allergy and Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/karger.com\/article-pdf\/doi\/10.1159\/000550719","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,20]],"date-time":"2026-03-20T17:00:55Z","timestamp":1774026055000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000550719"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,1,27]]},"references-count":36,"URL":"https:\/\/doi.org\/10.1159\/000550719","archive":["Portico"],"relation":{},"ISSN":["1018-2438","1423-0097"],"issn-type":[{"value":"1018-2438","type":"print"},{"value":"1423-0097","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,1,27]]}}}