{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T22:20:11Z","timestamp":1790288411938,"version":"4.1.0"},"reference-count":80,"publisher":"S. Karger AG","issue":"1","license":[{"start":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T00:00:00Z","timestamp":1770940800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T00:00:00Z","timestamp":1770940800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Neuroimmunomodulation"],"abstract":"<jats:p>Introduction: Sleep deficiency is known to increase the risk for multiple disease conditions involving immunopathology, in which inflammation is thought to be a mechanism for disease development. Thus, one potential way to mitigate negative health consequences of deficient sleep is to target inflammation. We investigated whether low-dose acetylsalicylic acid (ASA, aspirin) administration prior to and during exposure to an experimental sleep restriction challenge affects cellular immune responses to sleep restriction. Methods: We studied 46 healthy humans (19F\/27M, age range 19\u201363 years) in a randomized trial with 3 protocols each consisting of a 14-day at-home phase followed by an 11-day (10-night) in-laboratory stay (sleep restriction\/ASA, sleep restriction\/placebo, control sleep\/placebo) with daily ASA (81 mg\/day) or placebo intake across the entire study period (at-home and in-laboratory). During in-laboratory stays, sleep opportunity under both sleep restriction conditions was 8 h during 2 pre-challenge nights, 4 h during 5 nights of restricted sleep, and 8 h during 3 nights of recovery sleep. Under the control sleep condition, participants had a sleep opportunity of 8 h\/night throughout the protocol. Blood samples were analyzed prior to and following 5 nights of sleep restriction\/control sleep, and after 2 nights of recovery sleep. Data were analyzed using generalized linear mixed models. Results: Experimental sleep restriction increased WBC, lymphocyte, monocyte, eosinophil, basophil, CD4 T-cell counts, and the CD4\/CD8 T-cell ratio compared to control sleep under placebo (p &lt; 0.01). Low-dose ASA had no effect at pre-challenge for most cell types. However, low-dose ASA attenuated the eosinophil and basophil responses to sleep restriction and reduced elevation of the CD4\/CD8 T-cell ratio (p &lt; 0.01). Monocyte counts stayed elevated after 2 nights of recovery sleep in the sleep restriction\/ASA condition compared to control sleep, whereas monocyte counts recovered under placebo intake (p &lt; 0.01). Conclusion: The present study shows that low-dose ASA can counteract certain cellular immune responses to sleep restriction, in particular elevations in eosinophil and basophil counts as well as the CD4\/CD8 T-cell ratio, while not affecting most immune cell counts prior to the sleep restriction challenge.<\/jats:p>","DOI":"10.1159\/000551037","type":"journal-article","created":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T17:00:51Z","timestamp":1771002051000},"page":"125-137","source":"Crossref","is-referenced-by-count":1,"title":["The Effect of Low-Dose Acetylsalicylic Acid on Cellular Immune Responses to Experimental Sleep Restriction in Healthy Humans"],"prefix":"10.1159","volume":"33","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8912-2271","authenticated-orcid":false,"given":"Larissa 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