{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,8]],"date-time":"2026-05-08T16:15:00Z","timestamp":1778256900279,"version":"3.51.4"},"reference-count":24,"publisher":"S. Karger AG","issue":"2","license":[{"start":{"date-parts":[[2026,3,20]],"date-time":"2026-03-20T00:00:00Z","timestamp":1773964800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"},{"start":{"date-parts":[[2026,3,20]],"date-time":"2026-03-20T00:00:00Z","timestamp":1773964800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Fetal Diagn Ther"],"abstract":"<jats:p>Introduction: In this study, we aimed to investigate the expression of nuclear Yes-associated protein (nYAP), a molecule involved in mechanotransduction, during fetal lung development in a novel ex vivo mouse model of congenital diaphragmatic hernia (CDH) lung hypoplasia. Methods: Pregnant CD1 mice were gavaged nitrofen at E8.5 to induce CDH lung hypoplasia. At E16.5, fetal lungs were harvested and cultured ex vivo for 72 h with or without tracheal occlusion (hypo-TO and hypo, respectively). Age-matched normal fetal lung controls (norm-TO and norm) were evaluated in parallel. Whole lungs at E16.5 + 3 were analyzed for nYAP and markers of distal epithelial differentiation by immunohistochemistry and quantitative gene expression. Results: There was robust nYAP expression in norm-TO lungs. Analysis of the distal lung parenchyma in normal and hypoplastic lungs showed enhanced epithelial nYAP expression in the distal airways of both norm-TO and hypo-TO lungs relative to their respective controls. Hypo lungs had the lowest expression of nYAP among the groups. There was significantly increased expression of both Ctgf and Cyr61 in hypo-TO lungs compared to hypo lungs without tracheal occlusion (Ctgf: 1.57 \u00b1 0.43 and 1.02 \u00b1 0.23, p = 0.016; Cyr61: 1.60 \u00b1 0.34 and 1.01 \u00b1 0.17, p = 0.003). Conclusion: In this ex vivo model, tracheal occlusion upregulates nYAP in both control and hypoplastic lungs during the canalicular stage of development, suggesting a critical role of the mechanosensory response molecule in CDH lung hypoplasia.<\/jats:p>","DOI":"10.1159\/000551625","type":"journal-article","created":{"date-parts":[[2026,3,20]],"date-time":"2026-03-20T17:00:53Z","timestamp":1774026053000},"page":"180-187","source":"Crossref","is-referenced-by-count":0,"title":["Nuclear Yes-Associated Protein Activation in an ex vivo Murine Lung Model of Fetal Tracheal Occlusion"],"prefix":"10.1159","volume":"53","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5799-1832","authenticated-orcid":false,"given":"Anne M.","family":"Sescleifer","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shelby R.","family":"Sferra","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Annalise B.","family":"Penikis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-9228-234X","authenticated-orcid":false,"given":"Wenyu","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juan C.","family":"Biancotti","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Latoya","family":"Stewart","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9229-4733","authenticated-orcid":false,"given":"Shaun M.","family":"Kunisaki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2026,3,20]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpedsurg.2020.06.022"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1038\/s41572-022-00362-w"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1053\/j.semperi.2019.07.003"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1186\/s10397-018-1041-9"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1016\/0022-3468(93)90426-l"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1002\/uog.26164"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.3389\/fped.2017.00295"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.00484.2020"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.3389\/fped.2021.780166"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1165\/rcmb.2019-0300ED"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1165\/rcmb.2019-0218OC"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1165\/rcmb.2024-0323OC"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpedsurg.2024.07.041"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1016\/j.jss.2018.06.064"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpedsurg.2014.01.013"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2014.06.003"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1242\/dev.166454"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1242\/dev.175810"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.200812-1827OC"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpedsurg.2010.02.007"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1016\/j.jss.2007.07.019"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1242\/dev.163105"},{"key":"ref23","doi-asserted-by":"publisher","DOI":"10.1002\/pd.6428"},{"key":"ref24","doi-asserted-by":"publisher","DOI":"10.1007\/s00383-022-05340-7"}],"container-title":["Fetal Diagnosis and Therapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/karger.com\/article-pdf\/doi\/10.1159\/000551625","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,8]],"date-time":"2026-05-08T16:01:19Z","timestamp":1778256079000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000551625"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,3,20]]},"references-count":24,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2026,5,8]]},"published-print":{"date-parts":[[2026,5,5]]}},"URL":"https:\/\/doi.org\/10.1159\/000551625","archive":["Portico"],"relation":{"is-supplemented-by":[{"id-type":"doi","id":"10.6084\/m9.figshare.31812061","asserted-by":"subject"}]},"ISSN":["1015-3837","1421-9964"],"issn-type":[{"value":"1015-3837","type":"print"},{"value":"1421-9964","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,3,20]]}}}