{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T16:11:07Z","timestamp":1780071067772,"version":"3.54.0"},"reference-count":23,"publisher":"S. Karger AG","license":[{"start":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T00:00:00Z","timestamp":1777680000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"},{"start":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T00:00:00Z","timestamp":1777680000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Acta Cytologica"],"abstract":"<jats:p>Introduction: Timely molecular testing is essential for guiding targeted therapy in non-small cell lung cancer (NSCLC), yet results are often delayed by limited tissue availability and reliance on formalin-fixed, paraffin-embedded (FFPE) material for next-generation sequencing (NGS). Cytology fine-needle aspiration specimens frequently provide the sole diagnostic sample, and their residual supernatants \u2013 typically discarded \u2013 represent an untapped source of tumor-derived nucleic acids. Methods: In this pilot study, cytology supernatants from 10 NSCLC cases with known EGFR or KRAS mutations by NGS were analyzed using Idylla\u2122 ctEGFR and ctKRAS Mutation Assays. Each 2\u20133 mL supernatant sample, stored in CytoLyt\u00ae at 4\u00b0C after cell block preparation, was tested without extraction. Idylla\u2122 results were compared with orthogonal NGS data for concordance, and turnaround times for routine FFPE-based testing were evaluated to model potential workflow improvements. Results: All four EGFR-mutated cases (p.L858R, p.G719A, and exon 19 deletions) were correctly detected by Idylla\u2122, yielding 100% concordance with NGS. Among six KRAS-mutated cases, Idylla\u2122 identified all p.G12C variants but missed p.G12V and p.Q61K, achieving 80% overall concordance. Median time from collection to NGS report was 22 days, whereas modeled same-day Idylla\u2122 testing offered a theoretical reduction of approximately 20 days in turnaround time. Discordant cases correlated with relatively low tumor cellularity in cell blocks. Conclusion: Cytology supernatants preserved in CytoLyt\u00ae are a feasible substrate for rapid, cartridge-based molecular testing using the Idylla\u2122 platform. This approach can serve as a complementary or rescue strategy when cellular slide preparations or FFPE tissue is limited or NGS fails, enabling faster access to actionable molecular results in NSCLC.<\/jats:p>","DOI":"10.1159\/000552356","type":"journal-article","created":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T16:00:40Z","timestamp":1777737640000},"page":"1-10","source":"Crossref","is-referenced-by-count":0,"title":["From Discard to Discovery: Leveraging Residual Cytology Fine-Needle Aspiration Supernatants for Rapid EGFR and KRAS Mutation Detection in Lung Cancer"],"prefix":"10.1159","author":[{"given":"Margaret","family":"Parham","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saroja Devi","family":"Geetha","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6351-5552","authenticated-orcid":false,"given":"David E.","family":"Ost","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sravanthi","family":"Lavu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4817-458X","authenticated-orcid":false,"given":"Gloria Hopkins","family":"Sura","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"127","published-online":{"date-parts":[[2026,5,2]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.5858\/arpa.2017-0388-cp"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1136\/jclinpath-2021-207597"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1158\/1535-7163.mct-21-0201"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1002\/dc.24548"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.3390\/cells9112486"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1111\/cyt.13475"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.3892\/ol.2025.15121"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1016\/j.lungcan.2018.05.024"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1093\/ajcp\/aqab121"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1111\/cyt.13406"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1056\/nejmoa2103695"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1002\/dc.24378"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1002\/cncy.22925"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1002\/cncy.21897"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2014.3741"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1093\/annonc\/mdv208"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1093\/annonc\/mdz102"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2019.00612"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1002\/cncy.22273"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1016\/j.jasc.2022.09.003"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1016\/j.pathol.2023.08.002"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1002\/cncy.22930"},{"key":"ref23","doi-asserted-by":"publisher","DOI":"10.1002\/cncy.21664"}],"container-title":["Acta Cytologica"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/karger.com\/article-pdf\/doi\/10.1159\/000552356","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T16:00:54Z","timestamp":1780070454000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000552356"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,5,2]]},"references-count":23,"URL":"https:\/\/doi.org\/10.1159\/000552356","archive":["Portico"],"relation":{},"ISSN":["0001-5547","1938-2650"],"issn-type":[{"value":"0001-5547","type":"print"},{"value":"1938-2650","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,5,2]]}}}