{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T16:11:00Z","timestamp":1784131860463,"version":"3.55.0"},"reference-count":26,"publisher":"S. Karger AG","license":[{"start":{"date-parts":[[2026,5,22]],"date-time":"2026-05-22T00:00:00Z","timestamp":1779408000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,5,22]],"date-time":"2026-05-22T00:00:00Z","timestamp":1779408000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Med Princ Pract"],"abstract":"<jats:p>Objective: To perform a histopathological analysis of the effects of mesenchymal stem cells (MSCs) and their osteogenically differentiated derivatives (Dif-MSCs) on reparative processes in bone tissue using an experimental model of fracture nonunion. Subjects and Methods: Fifteen male rabbits aged 3 months were randomly assigned to three groups: control with induced femoral nonunion (n = 5), nonunion treated with adipose-derived MSCs (n = 5), and nonunion treated with Dif-MSCs (n = 5). MSCs were isolated from lipoaspirate, cultured to passage 3, and differentiated osteogenically for 4 weeks. A 5-mm bone defect was created surgically, and cells were injected into the defect zone on day 7. Animals were euthanized at 6 weeks postinjection. Bone specimens underwent fixation, decalcification, histological staining (hematoxylin-eosin and Van Gieson), and quantitative analysis of osteoblasts and osteocytes using ImageJ. Statistical analysis included one-way ANOVA and Welch\u2019s t test. Results: Macroscopic and histological evaluation revealed a gradation in regeneration: control group showed predominant fibrous tissue with immature bone and inflammation; MSCs group exhibited fibrocartilaginous callus with immature bone, bone marrow formation, and increased osteoblasts (52.0 \u00b1 16.2 per 10 HPF vs. control 28.6 \u00b1 7.0, p = 0.032); Dif-MSCs group demonstrated mature lamellar bone, organized osteons, mature bone marrow, and higher osteocytes (vs. MSCs, p = 0.006). ANOVA indicated trends in osteoblast increases (p = 0.092) and significant osteocyte variability (p = 0.014). Conclusion: Undifferentiated MSCs enhance early osteogenesis via endochondral ossification, while Dif-MSCs promote advanced maturation through mixed intramembranous and endochondral pathways, suggesting superior efficacy for treating fracture nonunion.<\/jats:p>","DOI":"10.1159\/000552577","type":"journal-article","created":{"date-parts":[[2026,5,22]],"date-time":"2026-05-22T16:00:50Z","timestamp":1779465650000},"page":"1-12","source":"Crossref","is-referenced-by-count":0,"title":["Histopathological Analysis of Adipose-Derived Mesenchymal Stem Cells and Their Osteogenic Derivatives in Promoting Bone Regeneration in a Model of Fracture Nonunion"],"prefix":"10.1159","author":[{"given":"Serhii","family":"Maslennikov","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maksym","family":"Danukalo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariia","family":"Isachenko","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuliya","family":"Avramenko","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maksym","family":"Holovakha","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yurii","family":"Kolesnyk","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"127","published-online":{"date-parts":[[2026,5,22]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1016\/j.injury.2020.02.030"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.3390\/bioengineering10010085"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1136\/military-2024-002863"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.2147\/CIA.S241936"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1186\/s13287-022-03054-0"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1016\/bs.mcb.2022.04.011"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.2174\/011574888X263333231218065453"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.15797"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1002\/sctm.17-0133"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-020-03315-5"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.b.35516"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1155\/2021\/9919361"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1007\/s13770-017-0092-8"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1016\/j.tice.2022.101768"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.7717\/peerj.17616"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1038\/s41392-025-02313-9"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-0716-4510-9_8"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1186\/s13018-024-04565-7"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1093\/stcltm\/szac052"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.3389\/fbioe.2022.942128"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1038\/s41421-024-00689-6"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.3390\/cells10081925"},{"key":"ref23","doi-asserted-by":"publisher","DOI":"10.3389\/fmolb.2024.1268019"},{"key":"ref24","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-021-03517-x"},{"key":"ref25","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2023.125924"},{"key":"ref26","doi-asserted-by":"publisher","DOI":"10.1007\/s13770-024-00683-9"}],"container-title":["Medical Principles and Practice"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/karger.com\/article-pdf\/doi\/10.1159\/000552577","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T16:00:49Z","timestamp":1784131249000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000552577"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,5,22]]},"references-count":26,"URL":"https:\/\/doi.org\/10.1159\/000552577","archive":["Portico"],"relation":{},"ISSN":["1011-7571","1423-0151"],"issn-type":[{"value":"1011-7571","type":"print"},{"value":"1423-0151","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,5,22]]}}}