{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T17:00:38Z","timestamp":1784826038539,"version":"3.55.0"},"reference-count":43,"publisher":"S. Karger AG","license":[{"start":{"date-parts":[[2026,5,28]],"date-time":"2026-05-28T00:00:00Z","timestamp":1779926400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"},{"start":{"date-parts":[[2026,5,28]],"date-time":"2026-05-28T00:00:00Z","timestamp":1779926400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Neuroendocrinology"],"abstract":"<jats:p>Introduction: Postpartum depression (PPD) is a common and debilitating mood disorder that affects many mothers worldwide, presenting significant challenges to maternal and infant health. This study investigated the clinical relevance and functional mechanisms of miR-335-5p in PPD to identify novel therapeutic strategies. Methods: miR-335-5p expression was measured by RT-qPCR. Diagnostic value and clinical correlations of miR-335-5p were evaluated using ROC curve analysis, Spearman correlation, and logistic regression. A PPD mouse model was established to investigate the effects of miR-335-5p on depressive-like behaviors. Oxidative stress markers and inflammatory factors were assessed using commercial detection kits and ELISA, respectively. Potential target genes of miR-335-5p were predicted via online databases and further subjected to GO and KEGG enrichment analyses. Results: Serum miR-335-5p was upregulated in patients with PPD and showed high diagnostic accuracy (AUC = 0.862). Its expression correlated with sleep disorders, and both were identified as PPD risk factors. Similarly, miR-335-5p was elevated in a PPD mouse model. Knockdown of miR-335-5p alleviated depressive-like behaviors, including reduced rearing episodes, decreased total distance, increased feeding latency, and prolonged immobility time. Furthermore, miR-335-5p inhibition increased catalase activity, glutathione levels, and total antioxidant capacity, while reducing TNF-\u03b1, IL-6, and IL-1\u03b2 levels. The targets of miR-335-5p were enriched in the circadian rhythm and MAPK signaling pathways. Conclusion: Elevated miR-335-5p expression represents a potential biomarker for PPD and is associated with sleep disorders. In mouse models, miR-335-5p knockdown alleviates depressive-like behaviors, decreases oxidative stress, and inhibits inflammatory responses, thereby offering mechanistic insights into PPD.<\/jats:p>","DOI":"10.1159\/000552716","type":"journal-article","created":{"date-parts":[[2026,5,28]],"date-time":"2026-05-28T16:01:56Z","timestamp":1779984116000},"page":"1-12","source":"Crossref","is-referenced-by-count":0,"title":["Association of miR-335-5p Dysregulation with Postpartum Depression: Clinical Findings and Experimental Validation in a Mouse 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