{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T16:58:46Z","timestamp":1785257926528,"version":"3.55.0"},"reference-count":72,"publisher":"S. Karger AG","issue":"1","license":[{"start":{"date-parts":[[2026,5,30]],"date-time":"2026-05-30T00:00:00Z","timestamp":1780099200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,5,30]],"date-time":"2026-05-30T00:00:00Z","timestamp":1780099200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Kidney Dis"],"abstract":"<jats:p>Introduction: Vascular endothelial injury is a critical driver of renal dysfunction and glomerulosclerosis in chronic kidney disease (CKD). While endothelial damage-induced inflammation contributes to sclerosis, the underlying mechanisms remain unclear. Although miR-214 has been implicated in renal fibrosis, its role in vascular endothelial cells during glomerulosclerosis is undefined. Methods: In this study, we investigated miR-214 expression and function in renal endothelial cells using five-sixths nephrectomy (5\/6Nx) mice, endothelial-specific and smooth muscle-specific miR-214 knockout models, CD31-enriched primary renal endothelial cells, and mouse aortic endothelial cells. Transcriptome analysis, bioinformatic prediction, luciferase reporter assays, cytokine profiling, and pro-inflammatory stimulation experiments were performed to evaluate the regulatory relationship between miR-214 and RELA-associated inflammatory signaling. Results: We observed significant miR-214 upregulation in renal endothelial cells of 5\/6Nx mice. Endothelial-specific knockout of miR-214 aggravated glomerulosclerosis and endothelial dysfunction, whereas smooth muscle-specific knockout showed no significant effect. Transcriptome analysis revealed that miR-214 inhibition altered the NF-\u03baB pathway, specifically upregulating RELA. Bioinformatic prediction and luciferase reporter assays supported a direct interaction between miR-214 and the RELA 3\u2032-UTR in vitro. In CD31-enriched primary renal endothelial cells, modulation of miR-214 levels inversely regulated RELA expression. Cytokine profiling showed that miR-214 suppression enhanced pro-inflammatory secretion. Furthermore, pro-inflammatory stimulation experiments demonstrated that inhibition of miR-214 amplified RELA-associated endothelial inflammatory activation, while miR-214 overexpression attenuated it. Conclusion: Collectively, these results suggest that endothelial miR-214 protects against glomerulosclerosis by restraining RELA-associated inflammatory signaling, highlighting endothelial-targeted modulation of miR-214 as a potential therapeutic strategy for CKD-related glomerular injury.<\/jats:p>","DOI":"10.1159\/000552823","type":"journal-article","created":{"date-parts":[[2026,5,30]],"date-time":"2026-05-30T16:00:33Z","timestamp":1780156833000},"page":"600-621","source":"Crossref","is-referenced-by-count":0,"title":["Enhanced miR-214 in Vascular Endothelial Cells Retards Renal Inflammation and Glomerular Sclerosis in Five-Sixths Nephrectomy 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