{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T16:59:29Z","timestamp":1790269169333,"version":"4.1.0"},"reference-count":24,"publisher":"S. Karger AG","license":[{"start":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T00:00:00Z","timestamp":1786406400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"},{"start":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T00:00:00Z","timestamp":1786406400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Blood Purif"],"abstract":"<jats:p>Introduction: Endotoxin removal therapy using a polymyxin B-immobilized fiber column (PMX) has been reported to improve hemodynamics in patients with septic shock. However, the mechanisms underlying the blood pressure-increasing effects of PMX treatment remain unclear, and factors other than endotoxin adsorption may be involved. This study focused on vasoconstrictive lipid mediators in the blood and comprehensively analyzed these mediators in patients with septic shock. Methods: This prospective observational study included 10 patients with septic shock who underwent PMX treatment. Blood samples were collected at multiple time points up to 120 min after PMX initiation, and 23 lipid mediators were measured by mass spectrometry. Hemodynamic changes were evaluated using the vasopressor dependency index (VDI). Furthermore, platelet-rich plasma from healthy subjects was incubated with PMX fibers in vitro to assess platelet adhesion by electron microscopy and thromboxane production. Results: Among the 10 patients included, the plasma concentration of thromboxane B&lt;sub&gt;2&lt;\/sub&gt; (TXB&lt;sub&gt;2&lt;\/sub&gt;), a stable derivative of thromboxane A&lt;sub&gt;2&lt;\/sub&gt; (TXA&lt;sub&gt;2&lt;\/sub&gt;), was significantly higher at the PMX column outlet than at the inlet. This difference in TXB&lt;sub&gt;2&lt;\/sub&gt; concentration showed a significant positive correlation with platelet count. Patients with higher platelet counts tended to exhibit a greater decrease in VDI, although the difference was not statistically significant. In vitro experiments demonstrated that platelets adhered to PMX fibers, accompanied by morphological changes, and that the fibers stimulated TXB&lt;sub&gt;2&lt;\/sub&gt; production. Importantly, this production was suppressed by an antibody against the platelet surface antigen \u03b1IIb\u03b23. Conclusion: TXA&lt;sub&gt;2&lt;\/sub&gt;, produced by platelets upon contact with PMX fibers during treatment, may contribute to the rapid increase in blood pressure observed after initiation of therapy.<\/jats:p>","DOI":"10.1159\/000553006","type":"journal-article","created":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T16:00:41Z","timestamp":1786464041000},"page":"1-10","source":"Crossref","is-referenced-by-count":0,"title":["Possible Clinical Effects of Polymyxin B-Immobilized Fiber Columns via Platelet-Derived Lipid Mediators: A Prospective Observational Study"],"prefix":"10.1159","author":[{"given":"Shintaro","family":"Suzuki","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Satoshi","family":"Kazuma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroomi","family":"Tatsumi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshiki","family":"Masuda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0908-7718","authenticated-orcid":false,"given":"Yoko","family":"Koga","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroyuki","family":"Meguro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mie","family":"Kaino","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masakazu","family":"Shinohara","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"127","published-online":{"date-parts":[[2026,8,11]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2016.0287"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1097\/CCM.0000000000007075"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2016.0289"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1186\/s13054-023-04690-5"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1186\/cc13912"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1007\/s00134-015-3751-z"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2018.14618"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1016\/S2213-2600(26)00047-0"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2009.856"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1159\/000513213"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1007\/s00134-017-5004-9"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1097\/CCM.0000000000002014"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1097\/MCO.0000000000000633"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00024.2014"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1016\/j.pharmthera.2008.01.001"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1016\/j.bja.2018.12.019"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1186\/s13613-017-0337-7"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0119437"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1155\/2019\/9438750"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1160\/TH14-12-1067"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0163531"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1016\/j.thromres.2015.09.008"},{"key":"ref23","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20050237"},{"key":"ref24","doi-asserted-by":"publisher","DOI":"10.1016\/j.mbplus.2022.100115"}],"container-title":["Blood Purification"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/karger.com\/article-pdf\/doi\/10.1159\/000553006","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T16:00:33Z","timestamp":1790265633000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000553006"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,11]]},"references-count":24,"URL":"https:\/\/doi.org\/10.1159\/000553006","archive":["Portico"],"relation":{},"ISSN":["0253-5068","1421-9735"],"issn-type":[{"value":"0253-5068","type":"print"},{"value":"1421-9735","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,11]]}}}