{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,18]],"date-time":"2026-10-18T16:41:18Z","timestamp":1792341678812,"version":"4.4.0"},"reference-count":16,"publisher":"S. Karger AG","license":[{"start":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T00:00:00Z","timestamp":1789084800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"},{"start":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T00:00:00Z","timestamp":1789084800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Acta Haematol"],"abstract":"<jats:p>Introduction: Gene fusions involving core binding factors (CBFs), such as CBFB::MYH11, are major contributing factors to leukemia development. The pathogenic mechanism is believed to lie in abnormalities in CBF, however, myosin, the fusion partner, has received little attention. In a preliminary analysis, we identified a previously undescribed fusion transcript, CBFB::MYL11, in RNA-sequencing data from the T-cell leukemia cell line HPB-ALL. We hypothesized that leukemia cells harboring CBFB::MYH11 or CBFB::MYL11 may share a common pathological mechanism involving myosin fusion. Methods: Fluorescence in situ hybridization was performed to analyze the structure of CBFB::MYL11 and Western blotting was performed to verify the fusion protein in HPB-ALL. Differentially expressed gene and gene ontology (GO) analyses were performed on ME-1 harboring CBFB::MYH11 and HPB-ALL to investigate characteristics of gene expression and molecular function. Results: In situ amplification of MYL11 and co-amplification of CBFB and MYL11 on a marker chromosome were observed. Elevated MYH11 and MYL11 expression and significant upregulation of genes related to the cytoskeleton were observed in the ME-1 and HPB-ALL cell lines. Bands consistent with the CBFB::MYL11 fusion protein were observed using Western blotting. Conclusion: This study underscores the pathological significance of cytoskeletal abnormalities in leukemia with CBFB\/myosin fusion and provides a foundation for further investigation into their molecular mechanisms.<\/jats:p>","DOI":"10.1159\/aha\/abkag013","type":"journal-article","created":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T11:53:03Z","timestamp":1789127583000},"page":"1-1","source":"Crossref","is-referenced-by-count":0,"title":["T-Cell Leukemia Cell Line Harboring Previously Undescribed CBFB::MYL11 Fusion Exhibits a Genome Profile Implicating Cytoskeletal Abnormality"],"prefix":"10.1159","author":[{"given":"Tetsuko","family":"Kobayashi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Satsuki","family":"Matsushima","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroaki","family":"Ohnishi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"127","published-online":{"date-parts":[[2026,9,11]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2016-08-733196"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1182\/bloodadvances.2019000709"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2023.112373"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-44491-x"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1126\/science.aaf1709"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamcr.2009.07.006"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1158\/1535-7163.MCT-10-0730"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1038\/35087035"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1038\/ncb1160"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.3390\/biom12121862"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21103460"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2025.01.013"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1172\/JCI176311"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1126\/scisignal.aam6846"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0400272101"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1186\/s12967-017-1260-2"}],"container-title":["Acta Haematologica"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/karger.com\/article-pdf\/doi\/10.1159\/aha\/abkag013","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,18]],"date-time":"2026-10-18T16:00:53Z","timestamp":1792339253000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/aha\/abkag013"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,11]]},"references-count":16,"URL":"https:\/\/doi.org\/10.1159\/aha\/abkag013","archive":["Portico"],"relation":{"is-supplemented-by":[{"id-type":"doi","id":"10.6084\/m9.figshare.33513502","asserted-by":"subject"}]},"ISSN":["0001-5792","1421-9662"],"issn-type":[{"value":"0001-5792","type":"print"},{"value":"1421-9662","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,11]]}}}