{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T08:56:56Z","timestamp":1791190616253,"version":"4.1.0"},"reference-count":33,"publisher":"S. Karger AG","license":[{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Gynecol Obstet Invest"],"abstract":"<jats:p>Objectives: The aim of this study was to examine SEMA3C expression in endometriosis (EMs) and its relationship with ferroptosis in human endometrial stromal cells (HESCs). Design: This experimental study used patient tissue samples and in vitro gain- and loss-of-function assays. Participants\/Materials: The participants and materials involved endometrial tissues from 30 patients with EMs and 30 controls, including paired ectopic and eutopic tissues from 10 patients, and primary HESCs. Setting: This study was set in the Affiliated Women and Children\u2019s Hospital of Ningbo University and its associated laboratory facilities. Methods: SEMA3C expression was assessed by immunohistochemistry, Western blotting, and qRT-PCR in this study. Primary HESCs were subjected to SEMA3C overexpression or siRNA-mediated knockdown. Cell viability, migration, iron content, lipid peroxidation, antioxidant activity, inflammatory cytokines, and ferroptosis regulators were analyzed. Ferrostatin-1 was used for rescue experiments. Results: This experimental study showed that SEMA3C was upregulated in ectopic tissues versus controls (p &lt; 0.001) and correlated with disease severity (stages III\u2013IV vs. I\u2013II, p &lt; 0.01). Ectopic lesions showed higher SEMA3C mRNA than matched eutopic endometrium (p &lt; 0.001). Ectopic tissues and SEMA3C-overexpressing HESCs showed increased ferroptosis-related oxidative and inflammatory changes. SEMA3C overexpression enhanced HESC viability and migration, increased ACSL4, and decreased Nrf2, GPX4, and SLC7A11 expression (p &lt; 0.001). SEMA3C knockdown or Ferrostatin-1 attenuated these alterations and suppressed HESC viability and migration (p &lt; 0.01). Limitations: The findings of this study are based on clinical tissues and in vitro HESC experiments, and in vivo validation and prospective confirmation are required. Conclusions: It was shown thatSEMA3C promotes HESC viability and migration through ferroptosis-related oxidative and inflammatory mechanisms, identifying the SEMA3C-ferroptosis axis as a potential pathogenic pathway in EMs.<\/jats:p>","DOI":"10.1159\/goi\/aczag005","type":"journal-article","created":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T11:34:06Z","timestamp":1787052846000},"page":"1-1","source":"Crossref","is-referenced-by-count":0,"title":["SEMA3C Promotes the Progression of Endometriosis by Inducing Ferroptosis and Enhancing the Viability and Migration of Endometrial Stromal Cells"],"prefix":"10.1159","author":[{"given":"Yan","family":"Li","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"127","published-online":{"date-parts":[[2026,9,1]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra1810764"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1038\/s41574-019-0245-z"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1111\/1471-0528.17973"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.3389\/fmed.2025.1576490"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.3390\/ijms221910554"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1155\/2017\/7265238"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1111\/jog.13900"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1007\/s43032-020-00164-z"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1038\/s41420-022-00821-z"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2017.09.021"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2012.03.042"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1080\/15548627.2020.1810918"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1002\/mco2.70349"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1097\/MD.0000000000037421"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1038\/s41420-023-01606-8"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-023-06182-4"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.3390\/biomedicines6020042"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.3390\/ijms20030556"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1093\/hropen\/hoac009"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.3390\/ijms222010974"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1016\/s0015-0282(97)81391-x"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1016\/j.abb.2023.109535"},{"key":"ref23","doi-asserted-by":"publisher","DOI":"10.1016\/j.xcrm.2024.101596"},{"key":"ref24","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(21)00389-5"},{"key":"ref25","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2024.1417750"},{"key":"ref26","doi-asserted-by":"publisher","DOI":"10.15252\/emmm.201404922"},{"key":"ref27","doi-asserted-by":"publisher","DOI":"10.1002\/14651858.CD012179"},{"key":"ref28","doi-asserted-by":"publisher","DOI":"10.1038\/s41392-024-01887-0"},{"key":"ref29","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2023.113023"},{"key":"ref30","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2013.12.010"},{"key":"ref31","doi-asserted-by":"publisher","DOI":"10.1021\/ja411006a"},{"key":"ref32","doi-asserted-by":"publisher","DOI":"10.1503\/cmaj.220637"},{"key":"ref33","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-019-1730-1"}],"container-title":["Gynecologic and Obstetric Investigation"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/karger.com\/article-pdf\/doi\/10.1159\/goi\/aczag005","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T08:47:30Z","timestamp":1791190050000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/goi\/aczag005"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,1]]},"references-count":33,"URL":"https:\/\/doi.org\/10.1159\/goi\/aczag005","archive":["Portico"],"relation":{"is-supplemented-by":[{"id-type":"doi","id":"10.6084\/m9.figshare.33306894","asserted-by":"subject"}]},"ISSN":["0378-7346","1423-002X"],"issn-type":[{"value":"0378-7346","type":"print"},{"value":"1423-002X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,1]]}}}