{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,25]],"date-time":"2026-03-25T11:21:11Z","timestamp":1774437671415,"version":"3.50.1"},"reference-count":1,"publisher":"S. Karger AG","issue":"4","license":[{"start":{"date-parts":[[2002,1,1]],"date-time":"2002-01-01T00:00:00Z","timestamp":1009843200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2002,1,1]],"date-time":"2002-01-01T00:00:00Z","timestamp":1009843200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nephron"],"published-print":{"date-parts":[[2002]]},"abstract":"<jats:p>&lt;i&gt;Background\/Aims:&lt;\/i&gt; Growth of T cell lines from kidney graft biopsy specimens that suppress the antidonor response, either directly or through a soluble factor, both specific and nonspecific to donor, has been reported. Fine-needle aspiration biopsy sample cultures synthesize a large array of cytokines\/chemokines with significant differences between stable versus acute rejection transplants. We hypothesize that the products of such cultures may be endowed with antidonor response modulation abilities in kidney transplantation. &lt;i&gt;Methods:&lt;\/i&gt; From 46 patients, 21 rejection free (group A) and 25 developing 28 acute rejection crises (group B), samples were obtained on days 7 and 30 after transplantation in group A and on day 7 and on acute rejection day in group B. The supernatants obtained after 48 h of culture were studied for IL-1 receptor antagonist, IL-4, IL-4 soluble receptor alpha, IL-12, IL-13, IFN-\u03b3, TGF-\u03b21, TGF-\u03b22, GM-CSF, and PGE&lt;sub&gt;2&lt;\/sub&gt; and for their effects on mixed lymphocyte reactions, donor-recipient and recipient-third-party combinations. At the end, analysis by flow cytometry of donor-recipient cultures complemented the analysis done before cultures. &lt;i&gt;Results:&lt;\/i&gt; Day 7 supernatants from group A specifically and significantly inhibited the antidonor response; supernatants from group B nonspecifically stimulated the antidonor response. The IL-1 receptor antagonist production was significantly higher by day 7 in group A, and the PGE&lt;sub&gt;2&lt;\/sub&gt; production was significantly higher in group B. Flow cytometry analysis did not disclose significant differences between inhibited versus stimulated antidonor responses. &lt;i&gt;Conclusions:&lt;\/i&gt; Cultures of aspiration biopsy samples done early after transplantation in rejection-free patients produce soluble suppression-specific antidonor response factor(s), not present in cultures from biopsy specimens taken before and during rejection. This was associated with IL-1 receptor antagonist synthesis.<\/jats:p>","DOI":"10.1159\/000065025","type":"journal-article","created":{"date-parts":[[2003,4,15]],"date-time":"2003-04-15T21:05:17Z","timestamp":1050440717000},"page":"637-645","source":"Crossref","is-referenced-by-count":4,"title":["Cultures of Kidney Transplant Fine-Needle Aspiration Samples from Rejection-Free Patients Produce a Specific Antidonor Response Suppressive Factor"],"prefix":"10.1159","volume":"91","author":[{"given":"Jos\u00e9 Gerardo G.","family":"de Oliveira","sequence":"first","affiliation":[]},{"given":"Paula","family":"Xavier","sequence":"additional","affiliation":[]},{"given":"Jo\u00e3o","family":"Ramos","sequence":"additional","affiliation":[]},{"given":"Susana","family":"Sampaio","sequence":"additional","affiliation":[]},{"given":"Maria","family":"Magalh\u00e3es","sequence":"additional","affiliation":[]},{"given":"Armando","family":"Mendes","sequence":"additional","affiliation":[]},{"given":"Manuel","family":"Pestana","sequence":"additional","affiliation":[]}],"member":"127","published-online":{"date-parts":[[2002,7,15]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1755.1998.00800.x"}],"container-title":["Nephron"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/65025","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/65025","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T04:25:41Z","timestamp":1745468741000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000065025"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002]]},"references-count":1,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2002,8,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000065025","archive":["Portico"],"relation":{},"ISSN":["1660-8151","2235-3186"],"issn-type":[{"value":"1660-8151","type":"print"},{"value":"2235-3186","type":"electronic"}],"subject":[],"published":{"date-parts":[[2002]]}}}