{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T14:59:14Z","timestamp":1761058754414,"version":"3.40.4"},"reference-count":23,"publisher":"S. Karger AG","issue":"1","license":[{"start":{"date-parts":[[2008,1,1]],"date-time":"2008-01-01T00:00:00Z","timestamp":1199145600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2008,1,1]],"date-time":"2008-01-01T00:00:00Z","timestamp":1199145600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Urol Int"],"published-print":{"date-parts":[[2008]]},"abstract":"<i>Aim:<\/i> To study cell proliferation and DNA content in urothelial lesions identified after repeated intravesical instillations of mitomycin C (MMC) and bacillus Calmette-Gu\u00e9rin (BCG) in normal rat urothelium. <i>Material and Methods:<\/i> A total of 45 rats were divided into nine equal groups: those intravesically instilled with MMC; those receiving BCG intravesically, and a control group intravesically instilled with a physiological solution of sodium chloride (SF). Animals were killed 1, 4 or 8 weeks after the last intravesical instillation. An immunohistochemical streptavidin-biotin-peroxidase technique was performed to investigate Ki-67 expression and the DNA ploidy status was measured using static cytometry. <i>Results:<\/i> In urothelium exposed to MMC lesions such as simple hyperplasia, dysplasia, carcinoma in situ(CIS), and squamous cell metaplasia were identified. The proliferation index presented values of 11.73, 22.43 and 31.46% in hyperplasias, dysplasias, and CIS, respectively (p < 0.05). The frequency of abnormal DNA content amongst those animals exhibiting simple hyperplasias 25% were aneuploid, in the dysplasia 85.2% were aneuploid (p = 0.041). CIS were all multiploid, and squamous cell metaplasias were all diploid. Animals treated with BCG and SF presented no urothelial lesions and a diploid DNA content. <i>Conclusions:<\/i> The aneuploid and multiploid DNA content and proliferation index observed in urothelial lesions identified after repeated intravesical instillations of MMC reflect a high degree of genomic instability in such lesions which in itself may lead to rapid regeneration of new phenotypes.<\/jats:p>","DOI":"10.1159\/000111737","type":"journal-article","created":{"date-parts":[[2008,1,18]],"date-time":"2008-01-18T13:35:52Z","timestamp":1200663352000},"page":"90-97","source":"Crossref","is-referenced-by-count":3,"title":["Cell Proliferation and DNA Content in Rat Urothelial Lesions after Repeat Intravesical Instillations of Mitomycin C and Bacillus Calmette-Gu\u00e9rin"],"prefix":"10.1159","volume":"80","author":[{"given":"P.","family":"Oliveira","sequence":"first","affiliation":[]},{"given":"C.","family":"Palmeira","sequence":"additional","affiliation":[]},{"given":"A.","family":"Cola\u00e7o","sequence":"additional","affiliation":[]},{"given":"L.F.","family":"De la Cruz P","sequence":"additional","affiliation":[]},{"given":"C.","family":"Lopes","sequence":"additional","affiliation":[]}],"member":"127","published-online":{"date-parts":[[2008,1,18]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1093%2Fcarcin%2F19.12.2095"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1097%2F01.ju.0000162039.38023.5f"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1093%2Fjnci%2F83.10.682"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.urology.2003.11.049"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.2174%2F1381612003401019"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1016%2F0167-8140%2894%2901471-E"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1016%2FS0022-5347%2801%2964576-2"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1097%2F00000478-199812000-00001"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1097%2F01.ju.0000158123.33273.f9"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1002%2F1097-0142%2819810601%2947%3A11%3C2567%3A%3AAID-CNCR2820471109%3E3.0.CO%3B2-9"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1016%2F0278-6915%2895%2900042-Z"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1159%2F000227228"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1046%2Fj.1464-410X.2003.04360.x"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.urology.2003.11.049"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1007%2Fs004410050529"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1464-410X.1990.tb14927.x"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1002%2F1097-0142%2819930615%2971%3A12%3C3926%3A%3AAID-CNCR2820711221%3E3.0.CO%3B2-2"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1464-410X.1995.tb07269.x"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1016%2F0278-6915%2895%2900044-3"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1097%2F01.ju.0000073852.24341.4a"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1191%2F096032701718890577"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1002%2Fcyto.990220204"},{"key":"ref23","doi-asserted-by":"publisher","DOI":"10.1056%2FNEJM200104263441702"}],"container-title":["Urologia Internationalis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/111737","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/111737","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T02:19:24Z","timestamp":1745461164000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000111737"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008]]},"references-count":23,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2008,1,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000111737","archive":["Portico"],"relation":{},"ISSN":["0042-1138","1423-0399"],"issn-type":[{"type":"print","value":"0042-1138"},{"type":"electronic","value":"1423-0399"}],"subject":[],"published":{"date-parts":[[2008]]}}}