{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T19:55:50Z","timestamp":1760298950582,"version":"3.40.5"},"reference-count":34,"publisher":"S. Karger AG","issue":"3","license":[{"start":{"date-parts":[[2014,1,1]],"date-time":"2014-01-01T00:00:00Z","timestamp":1388534400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2014,1,1]],"date-time":"2014-01-01T00:00:00Z","timestamp":1388534400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Acta Cytologica"],"published-print":{"date-parts":[[2014]]},"abstract":"<b><i>Objective:<\/i><\/b> Lung cancer represents the leading cause of cancer death. <i>EGFR<\/i> mutations, detected<b> <\/b>in 10-40% of lung adenocarcinomas, are an essential key to therapeutic management. <i>EGFR<\/i>-activated mutations comprise mainly deletions in exon 19 and point mutations in exon 21. Although histology is the traditional method of detection,<b> <\/b>we investigated the role of cytology in <i>EGFR<\/i> mutations. <b><i>Study Design:<\/i><\/b> A total of 774 lung cancers were studied for <i>EGFR<\/i> mutations (676 histological and 98 cytological samples), including 424 adenocarcinomas, 326 non-small cell lung carcinomas not otherwise specified, and 24 squamous cell carcinomas. <b><i>Results:<\/i><\/b> We had a total of 164 (21.2%) cases of mutations. Common mutations were short in-frame deletions in exon 19 (53.7%) and single-nucleotide substitutions in exon 21 (34.1%); less frequent mutations included single-nucleotide substitutions in exon 18 (3.7%) and in-frame insertions\/deletions in exon 20 (8.5%). Histologically, <i>EGFR<\/i> mutations in exons 19 and 21 occurred in 19.4% and in exons 18 and 20 in 2.2%, while the rates cytologically were 13.3% for exons 19 and 21 and 5.1% for exons 18 and 20. <b><i>Conclusions:<\/i><\/b> The sensitivity for the detection of <i>EGFR<\/i> mutations in cytological samples overlaps histology, so the use of cytological material constitutes an adequate approach for treatment selection in patients with locally advanced or metastatic lung cancer.<\/jats:p>","DOI":"10.1159\/000363174","type":"journal-article","created":{"date-parts":[[2014,6,17]],"date-time":"2014-06-17T09:18:40Z","timestamp":1402996720000},"page":"275-280","source":"Crossref","is-referenced-by-count":7,"title":["Detection of Common and Less Frequent <b><i>EGFR<\/i><\/b> Mutations in Cytological Samples of Lung Cancer"],"prefix":"10.1159","volume":"58","author":[{"given":"Esther D.","family":"Rossi","sequence":"first","affiliation":[]},{"given":"Ren\u00ea","family":"Gerhard","sequence":"additional","affiliation":[]},{"given":"Luis","family":"Cirnes","sequence":"additional","affiliation":[]},{"given":"Jos\u00e9 Carlos","family":"Machado","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1006-6946","authenticated-orcid":false,"given":"Fernando","family":"Schmitt","sequence":"additional","affiliation":[]}],"member":"127","published-online":{"date-parts":[[2014,6,6]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc2088"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21166"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1093\/jjco\/hyp174"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1093\/annonc\/mdt205"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.2006.044347"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1002\/cncy.20197"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2010.31.8923"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.3747\/co.19.1058"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1097\/JTO.0b013e318206a221"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-08-1757"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1093\/jjco\/hyq087"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-06-1570"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-06-0714"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1002\/cncy.20151"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1038\/modpathol.2011.184"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1002\/cncy.20150"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1002\/cncy.20011"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjc.6604142"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa040938"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pmed.0020313"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1002\/dc.10039"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1159\/000325707"},{"key":"ref23","doi-asserted-by":"publisher","DOI":"10.1378\/chest.12-0350"},{"key":"ref24","doi-asserted-by":"publisher","DOI":"10.1002\/dc.21851"},{"key":"ref25","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.2007.051425"},{"key":"ref26","doi-asserted-by":"publisher","DOI":"10.1136\/jclinpath-2012-200773"},{"key":"ref27","doi-asserted-by":"publisher","DOI":"10.1136\/jclinpath-2011-200296"},{"key":"ref28","doi-asserted-by":"publisher","DOI":"10.1159\/000329029"},{"key":"ref29","doi-asserted-by":"publisher","DOI":"10.1309\/N5PQNGW2QKMX09X7"},{"key":"ref30","doi-asserted-by":"publisher","DOI":"10.1183\/09031936.00167407"},{"key":"ref31","doi-asserted-by":"publisher","DOI":"10.1111\/ajco.12177"},{"key":"ref32","doi-asserted-by":"publisher","DOI":"10.1097\/JTO.0b013e318189f579"},{"key":"ref33","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2303.2012.01000.x"},{"key":"ref34","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0042164"}],"container-title":["Acta 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