{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T17:40:18Z","timestamp":1775065218544,"version":"3.50.1"},"reference-count":9,"publisher":"S. Karger AG","issue":"6","license":[{"start":{"date-parts":[[2019,1,1]],"date-time":"2019-01-01T00:00:00Z","timestamp":1546300800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2019,1,1]],"date-time":"2019-01-01T00:00:00Z","timestamp":1546300800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Transfus Med Hemother"],"published-print":{"date-parts":[[2019]]},"abstract":"<b><i>Background:<\/i><\/b> The Forssman antigen (FORS1 Ag) is expressed on human red blood cells (RBCs). We investigated its presence on RBCs from Palestinian subjects and Swedish subjects by serological testing and by sequencing part of exon 7 of the<i> GBGT1<\/i> gene, which encodes Forssman synthase. \u00ad<b><i>Materials and Methods:<\/i><\/b> Blood samples from Palestinian subjects (<i>n<\/i> = 211 adults and <i>n<\/i> = 73 newborns) and from Swedish subjects (<i>n<\/i> = 47 adults) were analyzed in the study. RBCs from the Palestinian samples were typed for the FORS1 Ag using a monoclonal anti-Forssman antibody. The <i>GBGT1<\/i> gene was genotyped by DNA sequencing (all adult samples) or by using amplification refractory mutation system PCR (newborn samples). <b><i>Results:<\/i><\/b> All of the studied samples were negative for the FORS1 Ag by serologic typing. DNA sequencing of the 3\u2032 end of exon 7 of the <i>GBGT1<\/i> gene, which includes Arg296, showed that all samples had the wild-type Arg296 sequence, which is associated with an inactive form of Forssman synthase. We detected four single nucleotide polymorphisms in the adult samples; two were silent (p.Tyr232=, p.Gly290=), and two were missense (p.Arg243Cys, p.Arg243His). The allele frequencies ranged from 0.2 to 3.6%. The p.Arg243Cys SNP was a novel SNP that was detected in one Palestinian sample. <b><i>Conclusion:<\/i><\/b> Our results confirmed the allelic diversity of <i>GBGT1<\/i> and identified a novel nucleotide polymorphism in this gene, p.Arg243Cys. Our results also confirmed that the FORS blood group system is a low-frequency system.<\/jats:p>","DOI":"10.1159\/000497288","type":"journal-article","created":{"date-parts":[[2019,3,29]],"date-time":"2019-03-29T22:00:32Z","timestamp":1553896832000},"page":"450-454","source":"Crossref","is-referenced-by-count":3,"title":["Expression of the <b><i>GBGT1<\/i><\/b> Gene and the Forssman Antigen in Red Blood Cells in a Palestinian Population"],"prefix":"10.1159","volume":"46","author":[{"given":"Wafa\u00a0Ali","family":"Abusibaa","sequence":"first","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9333-4856","authenticated-orcid":false,"given":"Mahmoud\u00a0A.","family":"Srour","sequence":"additional","affiliation":[]},{"given":"Ali-Reza","family":"Moslemi","sequence":"additional","affiliation":[]},{"given":"Lola","family":"Svensson","sequence":"additional","affiliation":[]},{"given":"Carlos","family":"Jesus","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5205-8939","authenticated-orcid":false,"given":"Fernando","family":"Mendes","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8766-7104","authenticated-orcid":false,"given":"Camilla","family":"Hesse","sequence":"additional","affiliation":[]}],"member":"127","published-online":{"date-parts":[[2019,3,29]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.1987.27487264737.x"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2012-10-455055"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1038\/srep00975"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1111\/trf.12773"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.41.29390"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1093\/glycob\/cwm077"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1111\/trf.14813"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.74.7.3023"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1093\/glycob\/cwn117"}],"container-title":["Transfusion Medicine and Hemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/497288","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/497288","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T16:17:01Z","timestamp":1745425021000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000497288"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019]]},"references-count":9,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2019,12,3]]},"published-print":{"date-parts":[[2019,12,17]]}},"URL":"https:\/\/doi.org\/10.1159\/000497288","archive":["Portico"],"relation":{},"ISSN":["1660-3796","1660-3818"],"issn-type":[{"value":"1660-3796","type":"print"},{"value":"1660-3818","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019]]}}}