{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T19:07:53Z","timestamp":1790622473005,"version":"4.1.0"},"reference-count":35,"publisher":"Georg Thieme Verlag KG","issue":"08","funder":[{"name":"Ren\u00e9 Baron Foundation"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Thromb Haemost"],"published-print":{"date-parts":[[2003]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Hematologic involvement is the main feature of Argentine hemorrhagic fever (AHF), an endemo-epidemic disease caused by Junin virus (JV). Since endothelial dysfunction could play a role in AHF-altered hemostasis, we studied human umbilical vein endothelial cell (HUVEC) infection with a virulent (JVv) and a non-virulent (JVa) JV strain. Cells were infected by the two JV variants with no detectable apoptosis or cytopathic effect. Both viral variants up-regulated ICAM-1 and VCAM-1 levels, while von Willebrand factor (VWF) production was decreased. Prostacyclin (PGI2) release and decay accelerating factor (DAF) expression were greater in JVv- than in JVa-infected or control cells. Furthermore, nitric oxide (NO) production and endothelial nitric oxide synthase (eNOS) expression was only raised in JVv-infected supernatants. Significant NO and PGI2 values were also detected in AHF patient sera.<\/jats:p><jats:p>These data demonstrate that endothelial cell responses are triggered subsequently by JV infection, suggesting that such alterations play a major role in the pathogenesis of AHF and perhaps in other viral-induced hemorrhagic diseases.<\/jats:p>","DOI":"10.1160\/th02-09-0043","type":"journal-article","created":{"date-parts":[[2003,7,8]],"date-time":"2003-07-08T08:18:00Z","timestamp":1057652280000},"page":"326-334","source":"Crossref","is-referenced-by-count":38,"title":["Endothelial cell function alteration after Junin virus infection"],"prefix":"10.1160","volume":"90","author":[{"given":"Ricardo Martin","family":"Gomez","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roberto Gabriel","family":"Pozner","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Angela","family":"Lazzari","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lina Paola","family":"D\u2019Atri","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Soledad","family":"Negrotto","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Marisa","family":"Chudzinski-Tavassi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda Isabel","family":"Berr\u00eda","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mirta","family":"Schattner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"194","published-online":{"date-parts":[[2017,12,6]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"554","DOI":"10.4269\/ajtmh.1994.51.554","article-title":"Prevalence of infection with Junin virus in rodent populations in the epidemic area of Argentine hemorrhagic fever","volume":"51","author":"JN Mills","year":"1994","journal-title":"Am J Trop Med Hyg"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1016\/S0891-5520(20)30573-0","article-title":"Latin American hemorrhagic fever","volume":"8","author":"B Vainrub","year":"1994","journal-title":"Infect Dis Clin North Am"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"1216","DOI":"10.1016\/S0140-6736(79)92335-3","article-title":"Efficacy of immune plasma in treatment of Argentine haemorrhagic fever and association between treatment and a late neurological syndrome","volume":"2","author":"JI Maiztegui","year":"1979","journal-title":"Lancet"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1016\/S0140-6736(84)90299-X","article-title":"Importance of dose of neutralising antibodies in treatment of Argentine haemorrhagic fever with immune plasma","volume":"2","author":"DA Enria","year":"1984","journal-title":"Lancet"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1016\/0166-3542(94)90030-2","article-title":"Antiviral treatment of Argentine hemorrhagic fever","volume":"23","author":"DA Enria","year":"1994","journal-title":"Antiviral Res"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1086\/514211","article-title":"Protective efficacy of a live attenuated vaccine against Argentine hemorrhagic fever. AHF Study Group","volume":"177","author":"JI Maiztegui","year":"1998","journal-title":"J Infect Dis"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"1091","DOI":"10.1086\/514749","article-title":"Clinical case definitions for Argentine hemorrhagic fever","volume":"28","author":"LH Harrison","year":"1999","journal-title":"Clin Infect Dis"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"216","DOI":"10.1056\/NEJM197808032990502","article-title":"Argentine hemorrhagic fever. Alterations of the complement system and anti-Junin-virus humoral response","volume":"299","author":"MM de Bracco","year":"1978","journal-title":"N Engl J Med"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"368","DOI":"10.1055\/s-0038-1653782","article-title":"Early markers of blood coagulation and fibrinolysis activation in Argentine hemorrhagic fever","volume":"73","author":"MV Heller","year":"1995","journal-title":"Thromb Haemost"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"470","DOI":"10.4269\/ajtmh.1990.42.470","article-title":"A plasma inhibitor of platelet aggregation in patients with Argentine hemorrhagic fever","volume":"42","author":"D Cummins","year":"1990","journal-title":"Am J Trop Med Hyg"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"543","DOI":"10.1111\/j.1365-2141.1989.tb04321.x","article-title":"A plasma inhibitor of platelet aggregation in patients with Lassa fever","volume":"72","author":"D Cummins","year":"1989","journal-title":"Br J Haematol"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"552","DOI":"10.1002\/(SICI)1096-9071(199912)59:4<552::AID-JMV21>3.0.CO;2-A","article-title":"Lassa and Mopeia virus replication in human monocytes\/macrophages and in endothelial cells: different effects on IL-8 and TNF-alpha gene expression","volume":"59","author":"IS Lukashevich","year":"1999","journal-title":"J Med Virol"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1055\/s-0037-1613538","article-title":"Interaction of pathogens with the endothelium","volume":"89","author":"S Hippenstiel","year":"2003","journal-title":"Thromb Haemost"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"S268","DOI":"10.1097\/00003246-200205001-00016","article-title":"Role of the endothelium in viral hemorrhagic fevers","volume":"30","author":"CJ Peters","year":"2002","journal-title":"Crit Care Med"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"776","DOI":"10.1128\/iai.20.3.776-781.1978","article-title":"Replication of Dengue and Junin viruses in cultured rabbit and human endothelial cells","volume":"20","author":"BS Andrews","year":"1978","journal-title":"Infect Immun"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"1605","DOI":"10.1099\/0022-1317-78-7-1605","article-title":"Molecular characterization of attenuated Junin virus strains","volume":"78","author":"CG Albarino","year":"1997","journal-title":"J Gen Virol"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1159\/000150039","article-title":"Viral strain dependent differences in experimental Argentine hemorrhagic fever (Junin virus) infection of guinea pigs","volume":"29","author":"RH Kenyon","year":"1988","journal-title":"Intervirology"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"2745","DOI":"10.1172\/JCI107470","article-title":"Culture of human endothelial cells derived from umbilical veins. Identification by morphologic and immunologic criteria","volume":"52","author":"EA Jaffe","year":"1973","journal-title":"J Clin Invest"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1002\/jmv.10254","article-title":"Junin virus-induced astrocytosis is impaired by iNOS inhibition","volume":"69","author":"RM Gomez","year":"2003","journal-title":"J Med Virol"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"1125","DOI":"10.1099\/0022-1317-70-5-1125","article-title":"Junin virus monoclonal antibodies: characterization and cross-reactivity with other arenaviruses","volume":"70","author":"A Sanchez","year":"1989","journal-title":"J Gen Virol"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1126\/science.3856324","article-title":"Molecular cloning of the complementary DNA for human tumor necrosis factor","volume":"228","author":"AM Wang","year":"1985","journal-title":"Science"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1067\/mlc.2001.113659","article-title":"Effect of nitric oxide on megakaryocyte growth induced by thrombopoietin","volume":"137","author":"M Schattner","year":"2001","journal-title":"J Lab Clin Med"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"395","DOI":"10.1146\/annurev.biochem.67.1.395","article-title":"Biochemistry and genetics of von Willebrand factor","volume":"67","author":"JE Sadler","year":"1998","journal-title":"Annu Rev Biochem"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"2605","DOI":"10.1182\/blood.V93.8.2605","article-title":"Identification and characterization of endothelial glycoprotein Ib using viper venom proteins modulating cell adhesion","volume":"93","author":"L Tan","year":"1999","journal-title":"Blood"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"2660","DOI":"10.1182\/blood.V90.7.2660","article-title":"Human endothelial cells in culture and in vivo express on their surface all four components of the glycoprotein Ib\/IX\/V complex","volume":"90","author":"G Wu","year":"1997","journal-title":"Blood"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"1203","DOI":"10.1093\/infdis\/166.5.1203","article-title":"Increased tumor necrosis factor-alpha levels in Argentine hemorrhagic fever","volume":"166","author":"MV Heller","year":"1992","journal-title":"J Infect Dis"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/0165-5728(95)00099-N","article-title":"Virus-activated T cells regulate expression of adhesion molecules on endothelial cells in sites of infection","volume":"62","author":"O Marker","year":"1995","journal-title":"J Neuroimmunol"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"4226","DOI":"10.1128\/jvi.71.6.4226-4232.1997","article-title":"Activation of endothelial cells via antibody-enhanced Dengue virus infection of peripheral blood monocytes","volume":"71","author":"R Anderson","year":"1997","journal-title":"J Virol"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1089\/107999099314234","article-title":"Human endothelium as a source of multifunctional cytokines: molecular regulation and possible role in human disease","volume":"19","author":"G Krishnaswamy","year":"1999","journal-title":"J Interferon Cytokine Res"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"525","DOI":"10.1055\/s-0038-1653402","article-title":"Factor VIII: C and factor VIII R: Ag in Argentine hemorrhagic fever","volume":"46","author":"FC Molinas","year":"1981","journal-title":"Thromb Haemost"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"743","DOI":"10.1006\/bbrc.1998.9719","article-title":"VEGF-A induces expression of eNOS and iNOS in endothelial cells via VEGF receptor-2 (KDR)","volume":"252","author":"J Kroll","year":"1998","journal-title":"Biochem Biophys Res Commun"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"538","DOI":"10.1002\/(SICI)1097-4644(19970315)64:4<538::AID-JCB2>3.0.CO;2-S","article-title":"Inducible nitric oxide and prostacyclin productions are differently controlled by extracellular matrix and cell density in human vascular endothelial cells","volume":"64","author":"A Orpana","year":"1997","journal-title":"J Cell Biochem"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"2236","DOI":"10.1172\/JCI117221","article-title":"Regulation of nitric oxide synthesis by proinflammatory cytokines in human umbilical vein endothelial cells. Elevations in terahydrobiopterin levels enhance endothelial nitric oxide synthase specific activity","volume":"93","author":"P Rosenkranz-Weiss","year":"1994","journal-title":"J Clin Invest"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"1532","DOI":"10.1002\/eji.1830260719","article-title":"Altered expression of host-encoded complement regulators on human cytomegalo-virus-infected cells","volume":"26","author":"OB Spiller","year":"1996","journal-title":"Eur J Immunol"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"4376","DOI":"10.4049\/jimmunol.155.9.4376","article-title":"Host cell-derived complement control proteins CD55 and CD59 are incorporated into the virions of two unrelated enveloped viruses. Human T cell leukemia\/lymphoma virus type I (HTLV-I) and human cytomegalovirus (HCMV)","volume":"155","author":"GT Spear","year":"1995","journal-title":"J Immunol"}],"container-title":["Thrombosis and Haemostasis"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.thieme-connect.de\/products\/ejournals\/pdf\/10.1160\/TH02-09-0043.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,5,31]],"date-time":"2023-05-31T12:22:54Z","timestamp":1685535774000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.thieme-connect.de\/DOI\/DOI?10.1160\/TH02-09-0043"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003]]},"references-count":35,"journal-issue":{"issue":"08","published-online":{"date-parts":[[2017,12,6]]},"published-print":{"date-parts":[[2003]]}},"URL":"https:\/\/doi.org\/10.1160\/th02-09-0043","archive":["Portico","CLOCKSS"],"relation":{},"ISSN":["0340-6245","2567-689X"],"issn-type":[{"value":"0340-6245","type":"print"},{"value":"2567-689X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2003]]}}}