{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,9]],"date-time":"2026-01-09T03:29:40Z","timestamp":1767929380415,"version":"3.49.0"},"reference-count":0,"publisher":"Georg Thieme Verlag KG","issue":"06","funder":[{"DOI":"10.13039\/501100001868","name":"Taiwan National Science Council","doi-asserted-by":"crossref","award":["NSC91-2320-B002-152"],"award-info":[{"award-number":["NSC91-2320-B002-152"]}],"id":[{"id":"10.13039\/501100001868","id-type":"DOI","asserted-by":"crossref"}]},{"name":"National Health Research Institute MD Ph.D. \/ DDS Ph.D. Predoctoral Fellowship","award":["RE89M004C"],"award-info":[{"award-number":["RE89M004C"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Thromb Haemost"],"published-print":{"date-parts":[[2004]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Triflamp, a metalloproteinase isolated from Trimeresurus flavoviridis, inhibits heterotypic adhesion between platelets and neutrophils. Coincubation studies demonstrate that direct interaction of triflamp with neutrophils is sufficient to inhibit the formation of neutrophil-platelet complexes. Its anti-adhesive effect is in a concentrationand incubation time-dependent manner. Triflamp reduces the expression of P-selectin glycoprotein ligand-1 (PSGL-1) on neutrophils and glycoprotein (GP) Ib\u03b1 on platelets as probed by flow cytometry and Western blot. Moreover, triflamp disrupts P-selectin-mediated adhesion by cleaving PSGL-1 from the neutrophil surface.There are obvious differences regarding PSGL-1 proteolysis by triflamp and cathepsin G. Besides the NH2-terminus of PSGL-1, other sites are truncated by triflamp. The inhibitory effect of triflamp on PSGL-1 expression was prevented by pretreatment with a metalloproteinase inhibitor, phenanthroline. However, triflamptreated platelets fully keep the ability for binding to PAFor fMLP-stimulated neutrophils. Our results indicate that degradation of platelet GPIb\u03b1 by triflamp does not interfere with neutrophil-platelet adhesion. Its effect on neutrophil PSGL-1 appears to be a critical factor for its inhibition on neutrophilplatelet interaction.<\/jats:p>","DOI":"10.1160\/th-03-09-0586","type":"journal-article","created":{"date-parts":[[2018,10,24]],"date-time":"2018-10-24T20:36:15Z","timestamp":1540413375000},"page":"1177-1185","source":"Crossref","is-referenced-by-count":11,"title":["Triflamp, a snake venom metalloproteinase, reduces neutrophil-platelet adhesion through proteolysis of PSGL-1 but not glycoprotein Ib\u03b1"],"prefix":"10.1160","volume":"91","author":[{"given":"Yu-Lun","family":"Tseng","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chia-Jung","family":"Lee","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chun-Chieh","family":"Hsu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tur-Fu","family":"Huang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"194","published-online":{"date-parts":[[2017,12,2]]},"container-title":["Thrombosis and Haemostasis"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.thieme-connect.de\/products\/ejournals\/pdf\/10.1160\/TH-03-09-0586.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2018,10,24]],"date-time":"2018-10-24T20:36:37Z","timestamp":1540413397000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.thieme-connect.de\/DOI\/DOI?10.1160\/TH-03-09-0586"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004]]},"references-count":0,"aliases":["10.1160\/th03-09-0586"],"journal-issue":{"issue":"06","published-online":{"date-parts":[[2017,12,15]]},"published-print":{"date-parts":[[2004]]}},"URL":"https:\/\/doi.org\/10.1160\/th-03-09-0586","relation":{},"ISSN":["0340-6245","2567-689X"],"issn-type":[{"value":"0340-6245","type":"print"},{"value":"2567-689X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2004]]}}}